Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	997,179	C→T	A112T (GCC→ACC)	ssuE ←	NAD(P)H‑dependent FMN reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	997,179	0	C	T	100.0%	173.0 / NA	43	A112T (GCC→ACC)	ssuE	NAD(P)H‑dependent FMN reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/24);  total (19/24)

GGTTTGCAGATTTGGCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTG  >  NC_000913/997036‑997326                                                                                                                                                |                                                                                                                                                    ggTTTGCAGATTTGGCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTa                                                                                                                                              <  1:408602/151‑1 (MQ=255)          aTTTGGCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTc                                                                                                                                     <  1:321841/151‑1 (MQ=255)              ggCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGcc                                                                                                                                  >  1:259970/1‑150 (MQ=255)              ggCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCATGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCa                                                                                                                                 <  1:137979/151‑1 (MQ=255)                cGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGc                                                                                                                               >  1:420366/1‑151 (MQ=255)                  tGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCgg                                                                                                                             >  1:501988/1‑151 (MQ=255)                          ggTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGcacc                                                                                                                      >  1:473152/1‑150 (MQ=255)                              tgtgATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACttt                                                                                                                 >  2:397766/1‑151 (MQ=255)                                tgATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGc                                                                                                               >  2:467007/1‑151 (MQ=255)                                        aTCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCaaa                                                                                                       <  1:160218/151‑1 (MQ=255)                                           aaTTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAgcg                                                                                                    >  2:65081/1‑151 (MQ=255)                                               aCTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGt                                                                                                 <  2:558937/150‑1 (MQ=255)                                                cTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGt                                                                                                 <  1:449410/149‑1 (MQ=255)                                                  tgtgAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCt                                                                                              <  2:547785/150‑1 (MQ=255)                                                   gtgAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTgg                                                                                            <  1:56231/151‑1 (MQ=255)                                                             ggCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCg                                                                                  <  1:511759/151‑1 (MQ=255)                                                                aaaCACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGc                                                                               >  1:48250/1‑151 (MQ=255)                                                                 aaCACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGc                                                                               >  1:42667/1‑150 (MQ=255)                                                                 aaCACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGc                                                                               >  1:306774/1‑150 (MQ=255)                                                                       ccGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGttt                                                                        >  1:284707/1‑151 (MQ=255)                                                                             aGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACg                                                                  <  2:55565/151‑1 (MQ=255)                                                                                  ctcCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCg                                                             >  1:612267/1‑151 (MQ=255)                                                                                       gAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGaa                                                          <  1:232430/149‑1 (MQ=255)                                                                                       gAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAAt                                                         <  1:113590/150‑1 (MQ=255)                                                                                       gAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATa                                                        <  1:439493/151‑1 (MQ=255)                                                                                         gCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAgg                                                      <  1:61734/151‑1 (MQ=255)                                                                                             tCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAggcgg                                                   <  1:356661/150‑1 (MQ=255)                                                                                              cAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAggcggc                                                  <  2:274027/150‑1 (MQ=255)                                                                                                     cTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATac                                           >  1:666659/1‑150 (MQ=255)                                                                                                              ggTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGt                                   <  1:326496/149‑1 (MQ=255)                                                                                                                     gggCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAAt                          >  1:489130/1‑151 (MQ=255)                                                                                                                           aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    <  2:44264/151‑1 (MQ=255)                                                                                                                           aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    <  2:676061/151‑1 (MQ=255)                                                                                                                           aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    >  2:343082/1‑151 (MQ=255)                                                                                                                           aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    >  1:387231/1‑151 (MQ=255)                                                                                                                           aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    <  1:262063/151‑1 (MQ=255)                                                                                                                           aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGcc                     <  1:497230/150‑1 (MQ=255)                                                                                                                               gACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATc                 >  1:99474/1‑150 (MQ=255)                                                                                                                                aCCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCgg               <  1:14130/151‑1 (MQ=255)                                                                                                                                     cagcagATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGt          >  2:100878/1‑151 (MQ=255)                                                                                                                                          gATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGc       >  2:101076/1‑149 (MQ=255)                                                                                                                                             gggTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTg  <  2:260809/151‑1 (MQ=255)                                                                                                                                             gggTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTg  <  2:248812/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    GGTTTGCAGATTTGGCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTG  >  NC_000913/997036‑997326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑