Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,013,807	A→G	V183V (GTA→GTG)	pqiB →	paraquat‑inducible, SoxRS‑regulated MCE domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,013,807	0	A	G	100.0%	161.7 / NA	46	V183V (GTA→GTG)	pqiB	paraquat‑inducible, SoxRS‑regulated MCE domain protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

AAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGG  >  NC_000913/1013662‑1013954                                                                                                                                                  |                                                                                                                                                    aaGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTc                                                                                                                                                <  2:631179/151‑1 (MQ=255)  aGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCg                                                                                                                                               <  2:426502/151‑1 (MQ=255)        caAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGaa                                                                                                                                         <  2:230231/151‑1 (MQ=255)              ggATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAg                                                                                                                                    <  1:673972/150‑1 (MQ=255)              ggATAAATACGATTTGCTGGACTCGCAACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGc                                                                                                                                   <  1:284017/151‑1 (MQ=255)                aTAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCAc                                                                                                                                 >  1:158748/1‑151 (MQ=255)                 tAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCAcc                                                                                                                                >  2:83815/1‑151 (MQ=255)                  aaaTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCt                                                                                                                               <  1:99007/151‑1 (MQ=255)                  aaaTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCt                                                                                                                               <  1:2656/151‑1 (MQ=255)                    aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc                                                                                                                             >  1:66729/1‑151 (MQ=255)                    aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc                                                                                                                             <  1:511428/151‑1 (MQ=255)                    aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc                                                                                                                             <  1:135967/151‑1 (MQ=255)                       cGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGAt                                                                                                                          >  1:671209/1‑151 (MQ=255)                          tttGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATac                                                                                                                        >  2:313479/1‑150 (MQ=255)                           ttGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATaca                                                                                                                       <  1:19486/150‑1 (MQ=255)                              cTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACaa                                                                                                                    >  2:59336/1‑150 (MQ=255)                                ggACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACaaaaa                                                                                                                 <  1:548803/151‑1 (MQ=255)                                           cgctGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCTCCTTCGATACACAAAAACGCAATATCAg                                                                                                      <  1:451225/148‑1 (MQ=255)                                            gctGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGc                                                                                                     >  2:632779/3‑151 (MQ=255)                                              tGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCt                                                                                                    >  1:565571/1‑150 (MQ=255)                                                  cccGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCa                                                                                                >  2:72175/1‑150 (MQ=255)                                                    cGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCTCCTTCGATACACAAAAACGCAATATCAGCTATCAACt                                                                                             <  1:571217/151‑1 (MQ=255)                                                       cTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACt                                                                                             <  1:319338/148‑1 (MQ=255)                                                         gATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTtca                                                                                        <  2:191263/151‑1 (MQ=255)                                                            gCGAAGGGTATCCGTGTGATTTTCGATAGAAAAAAAGCCGGGCACCGCTCGCCAGGGGATCCGGTGCGGTTCCGTGCCAATCGGGTGGGTTCGGTTGAAACAAGCACTTTGGATAAACAAAAAGGCAATATCAGCTATCAACTGTccat                                                                                       >  2:224177/1‑149 (MQ=255)                                                            gCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTtcatca                                                                                     <  1:632798/151‑1 (MQ=255)                                                            gCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTtcatca                                                                                     <  1:401048/151‑1 (MQ=255)                                                                 aGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGTCTATCGGGTGGGTTCGGTTGAAACCAGCTCCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCa                                                                                <  2:88350/151‑1 (MQ=255)                                                                   gTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCAcc                                                                              >  2:596152/1‑151 (MQ=255)                                                                       ccGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTAt                                                                          <  1:680395/151‑1 (MQ=255)                                                                         gtgtGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATg                                                                         >  1:46674/1‑150 (MQ=255)                                                                             gATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCg                                                                     <  2:582890/150‑1 (MQ=255)                                                                              aTTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGAc                                                                   >  2:284484/1‑151 (MQ=255)                                                                                tctcGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACt                                                                  <  1:20036/150‑1 (MQ=255)                                                                                   cGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTg                                                                 <  1:279894/148‑1 (MQ=255)                                                                                     aTAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGAc                                                            <  1:391095/151‑1 (MQ=255)                                                                                     aTAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTACTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGAc                                                            >  2:483678/1‑151 (MQ=255)                                                                                              aaGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGt                                                   <  2:250154/151‑1 (MQ=255)                                                                                                gCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTc                                                 >  2:154581/1‑151 (MQ=255)                                                                                                     gCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTc                                            >  1:399042/1‑151 (MQ=255)                                                                                                           ctcGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGaa                                       >  1:403666/1‑150 (MQ=255)                                                                                                                        ccGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTg                         >  2:444115/1‑151 (MQ=255)                                                                                                                          ggTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCg                       <  1:521132/151‑1 (MQ=255)                                                                                                                                gTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGa                  <  2:284858/150‑1 (MQ=255)                                                                                                                                             cGGGTGGGTTCGGTTGAAACCAGCACCTTCGGTACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTcagc      >  1:401557/1‑149 (MQ=255)                                                                                                                                               ggTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAggg  <  2:24278/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    AAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGG  >  NC_000913/1013662‑1013954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑