Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,088,769	C→T	G363G (GGG→GGA)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,088,769	0	C	T	100.0%	162.0 / NA	42	G363G (GGG→GGA)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/15);  total (27/15)

TTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGTTTTCGTCATAAACGTAATCAAGATCGATAT  >  NC_000913/1088621‑1088905                                                                                                                                                     |                                                                                                                                         ttAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGtcc                                                                                                                                        >  2:288512/1‑151 (MQ=255)        gggATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCa                                                                                                                                 <  1:469684/151‑1 (MQ=255)             cTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCggg                                                                                                                            >  2:574755/1‑151 (MQ=255)              tAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCggg                                                                                                                            >  2:546695/1‑150 (MQ=255)               aaaTCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGAt                                                                                                                          >  1:397781/1‑151 (MQ=255)               aaaTCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCCTTGACCAGTCCATCACCATc                                                                                                                               <  2:396292/146‑1 (MQ=255)                aaTCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATc                                                                                                                         >  2:545036/1‑151 (MQ=255)                   cccAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGc                                                                                                                      >  2:357274/1‑151 (MQ=255)                     cAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCa                                                                                                                     >  1:168297/1‑150 (MQ=255)                                  gCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATa                                                                                                       <  2:237101/151‑1 (MQ=255)                                    aTCCACGCATAGATGTTTACACCTGAGCGGGGACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATac                                                                                                      >  1:145042/1‑150 (MQ=255)                                     tCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCGGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATacac                                                                                                    >  1:327439/1‑151 (MQ=255)                                     tCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATacac                                                                                                    >  2:321721/1‑151 (MQ=255)                                            aTAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGat                                                                                             <  2:121754/151‑1 (MQ=255)                                                    ttACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCa                                                                                      <  1:308585/150‑1 (MQ=255)                                                               cGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCAccc                                                                          <  2:468478/151‑1 (MQ=255)                                                                 ggTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGc                                                                        <  2:11413/151‑1 (MQ=255)                                                                  gTACGTAATTGCCAGTCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGcg                                                                       <  2:643682/151‑2 (MQ=255)                                                                    aCGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTg                                                                      <  1:401511/150‑1 (MQ=255)                                                                            gCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGcac                                                             >  1:451023/1‑151 (MQ=255)                                                                             ccAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGcac                                                             >  2:212841/1‑150 (MQ=255)                                                                                ggCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATc                                                          >  1:605204/1‑150 (MQ=255)                                                                                  cAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAat                                                       >  2:127458/1‑151 (MQ=255)                                                                                   aaCCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAat                                                       >  1:497089/1‑150 (MQ=255)                                                                                     cccGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATAtt                                                    <  1:441186/151‑1 (MQ=255)                                                                                     cccGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATAtt                                                    <  2:29263/151‑1 (MQ=255)                                                                                          cTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGAt                                               >  1:628069/1‑151 (MQ=255)                                                                                            aaaaaTATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATcc                                             >  1:257448/1‑151 (MQ=255)                                                                                               aaTATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCAtt                                          >  1:89995/1‑151 (MQ=255)                                                                                                   tCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCt                                      >  2:319917/1‑151 (MQ=255)                                                                                                   tCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCt                                      >  2:630103/1‑151 (MQ=255)                                                                                                      gCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTg                                     <  1:630164/149‑1 (MQ=255)                                                                                                        tttCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAgg                                 >  1:104915/1‑151 (MQ=255)                                                                                                         ttCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAgg                                 <  1:475158/150‑1 (MQ=255)                                                                                                         ttCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAg                                  <  2:366024/149‑1 (MQ=255)                                                                                                         ttCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGt                                >  1:96116/1‑151 (MQ=255)                                                                                                           cATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGttt                              >  1:622890/1‑151 (MQ=255)                                                                                                              tGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGTTTTCg                           >  1:575482/1‑151 (MQ=255)                                                                                                                  aGCAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGTTTTCGTCAt                       <  1:515978/151‑1 (MQ=255)                                                                                                                    cAAACGATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGTTTTCGTCATa                      >  1:653838/1‑150 (MQ=255)                                                                                                                         gATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGTTTTCGTCATAAACGt                 >  2:330553/1‑150 (MQ=255)                                                                                                                         gATTTGGAAACCAGACCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGTTTTCGTCATAAACGt                 >  1:489368/1‑150 (MQ=255)                                                                                                                                       aCCTCTTTGACCAGTCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGTTTTCGTCATAAACGTAATCAAGATCGatat  >  1:535233/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                         TTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATGCCTGCAAATACACGGTTGATATTTGCATATCTTTCACCCGCTGAATTAGCACATCAATATTGCGATCCATTTGCTGGAGGTTTTCGTCATAAACGTAATCAAGATCGATAT  >  NC_000913/1088621‑1088905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑