Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,163,578	G→A	A107T (GCA→ACA)	thiK →	thiamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,163,578	0	G	A	100.0%	129.8 / NA	34	A107T (GCA→ACA)	thiK	thiamine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/20);  total (14/20)

GCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGGCAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCAAA  >  NC_000913/1163430‑1163721                                                                                                                                                     |                                                                                                                                                gcAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACa                                                                                                                                               <  2:340545/151‑1 (MQ=255)    gTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGc                                                                                                                                            <  2:240819/151‑1 (MQ=255)    gTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGc                                                                                                                                            >  2:644895/1‑151 (MQ=255)               tGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCAGATACCAACGAACTGACAGGCTTGCTGtatt                                                                                                                                  >  2:239039/1‑150 (MQ=255)                           gggCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATcaaca                                                                                                                     <  1:314466/151‑1 (MQ=255)                                ttATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCAc                                                                                                                >  1:222442/1‑151 (MQ=255)                                 tATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCAc                                                                                                                <  2:416423/150‑1 (MQ=255)                                 tATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCAc                                                                                                                <  1:632915/150‑1 (MQ=255)                                 tATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCAc                                                                                                                <  1:21380/150‑1 (MQ=255)                                     acaACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGtttt                                                                                                           >  1:542833/1‑151 (MQ=255)                                        aCTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTtggc                                                                                                        <  1:577324/151‑1 (MQ=255)                                           aCCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTtggct                                                                                                       <  2:100789/149‑1 (MQ=255)                                            cccGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTtggctggc                                                                                                    >  2:228733/1‑151 (MQ=255)                                              cGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCg                                                                                                   >  1:431201/1‑150 (MQ=255)                                                 atgcatTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAAt                                                                                                >  1:504210/1‑150 (MQ=255)                                                    catTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACg                                                                                            >  2:463258/1‑151 (MQ=255)                                                      ttGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCt                                                                                          >  1:242667/1‑151 (MQ=255)                                                        gCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGt                                                                                        <  1:648234/151‑1 (MQ=255)                                                                 ccGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTAc                                                                               >  1:375689/1‑151 (MQ=255)                                                                    cATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTgg                                                                            <  2:77275/151‑1 (MQ=255)                                                                                                          gCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGAc                                       <  1:880/150‑1 (MQ=255)                                                                                                          gCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGAc                                       <  2:365184/150‑1 (MQ=255)                                                                                                                        aaaCGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGACGCGGCGGACAGTGGGTTGGCTGCg                        <  2:496124/151‑1 (MQ=255)                                                                                                                          aCGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGaa                      <  1:433845/151‑1 (MQ=255)                                                                                                                           cGTATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAAt                     <  2:261585/151‑1 (MQ=255)                                                                                                                             tATTTGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGt                   >  2:589078/1‑151 (MQ=255)                                                                                                                                 tGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTaa                >  1:474085/1‑150 (MQ=255)                                                                                                                                 tGCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTaa                <  1:234228/150‑1 (MQ=255)                                                                                                                                  gCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTaaaa              <  1:660691/151‑1 (MQ=255)                                                                                                                                  gCCGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTaaaa              <  1:43149/151‑1 (MQ=255)                                                                                                                                    cGGATACCAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTAAAACg            <  2:343733/151‑1 (MQ=255)                                                                                                                                          ccAACGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTgcg      >  2:550550/1‑151 (MQ=255)                                                                                                                                             aCGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCa    >  2:453913/1‑150 (MQ=255)                                                                                                                                              cGAACTGACAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCaaa  <  1:476131/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                GCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGGCAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCAAA  >  NC_000913/1163430‑1163721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑