| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,214,838 | T→C | E213G (GAA→GGA) | bluF ← | anti‑repressor for YcgE, blue light‑responsive; FAD‑binding; inactive c‑di‑GMP phosphodiesterase‑like EAL domain protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,214,838 | 0 | T | C | 98.1% | 185.2 / ‑3.6 | 52 | E213G (GAA→GGA) | bluF | anti‑repressor for YcgE, blue light‑responsive; FAD‑binding; inactive c‑di‑GMP phosphodiesterase‑like EAL domain protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (23/28); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
TTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGTTGGTGAAAGGGCA > NC_000913/1214692‑1214984 | tttCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCcccc > 1:616853/1‑151 (MQ=255) aTTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCt < 1:199480/151‑1 (MQ=255) tAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCttt > 2:591401/1‑151 (MQ=255) aaaaaaGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCt > 1:367571/1‑151 (MQ=255) aaaaaGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTg > 2:178030/1‑151 (MQ=255) gaCTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAAcc < 1:52959/151‑1 (MQ=255) gCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGc < 1:104292/149‑1 (MQ=255) gCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCt < 1:278519/150‑1 (MQ=255) cGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTAt > 1:565790/1‑151 (MQ=255) ggTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTg < 2:435095/151‑1 (MQ=255) gTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATggg < 2:349041/151‑1 (MQ=255) tAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCt > 1:121214/1‑151 (MQ=255) aaCCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCt < 1:357259/150‑1 (MQ=255) aGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCtt < 1:111232/151‑1 (MQ=255) gggTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTc < 2:487927/151‑1 (MQ=255) ggTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTc > 2:475317/1‑150 (MQ=255) cATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAtt < 2:4174/150‑1 (MQ=255) aTAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAtttt < 1:386131/151‑1 (MQ=255) tAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAttttt > 2:57890/1‑151 (MQ=255) aGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAtttttt > 2:137174/1‑151 (MQ=255) tAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGcac > 1:577774/1‑150 (MQ=255) tgattgaAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATgg < 1:611355/150‑1 (MQ=255) gattgaAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCt < 1:43179/151‑1 (MQ=255) tgaAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTc < 1:309080/150‑1 (MQ=255) aTCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTAt < 2:661825/150‑1 (MQ=255) tCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATcc > 2:464480/1‑151 (MQ=255) aGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCcgcg < 1:404189/151‑1 (MQ=255) gCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCcgcg > 1:318810/1‑150 (MQ=255) gCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCcgcg > 2:413990/1‑150 (MQ=255) cAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATa > 1:562565/1‑150 (MQ=255) tGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAggg > 1:131206/1‑150 (MQ=255) gtgtGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCg < 1:364143/151‑1 (MQ=255) gCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTAAGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACaa < 1:348755/151‑1 (MQ=255) aTCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAAt > 1:91748/1‑149 (MQ=255) tCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAgg > 2:511624/1‑151 (MQ=255) gCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAg < 1:177802/150‑1 (MQ=255) aaGCGCCTTCCTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCa < 2:438655/151‑1 (MQ=255) gcgcCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCTGCCGCGATAAGGGATCGACAATAGGATGAAAGGCaa > 2:26836/1‑150 (MQ=255) gcCTTACTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGt > 1:203178/1‑151 (MQ=255) aCTTTTGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTc < 1:87873/150‑1 (MQ=255) tttGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGt < 1:189734/149‑1 (MQ=255) tGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTtat > 1:131037/1‑151 (MQ=255) tGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTtat > 2:534896/1‑151 (MQ=255) tGAGATCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTta < 1:511638/150‑1 (MQ=255) gagaTCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTtat > 2:11829/1‑150 (MQ=255) aTCCGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGtt > 1:288564/1‑151 (MQ=255) ccGCTGTGTAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGTTg < 1:363536/150‑1 (MQ=255) gtgtAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGTTGGTGaaa < 2:532529/151‑1 (MQ=255) gtgtAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGTTGGTGa < 2:474128/149‑1 (MQ=255) tAGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGTTGGTGAAAggg > 2:12806/1‑151 (MQ=255) aGATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGTTGGTGAAAggg < 1:58889/150‑1 (MQ=255) gATTCCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGTTGGTGAAAGGGca < 1:645877/151‑1 (MQ=255) | TTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGCGATAAGGGATCGACAATAGGATGAAAGGCAAAGTGGTCGTTTATAGTTGGTGAAAGGGCA > NC_000913/1214692‑1214984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |