Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,225,326	C→T	G12G (GGG→GGA)	minD ←	inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,225,326	0	C	T	100.0%	151.0 / NA	39	G12G (GGG→GGA)	minD	inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/12);  total (27/12)

ATCGTAAACGACCCGGCGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATC  >  NC_000913/1225185‑1225468                                                                                                                                              |                                                                                                                                               aTCGTAAACGACCCGGCGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGccc                                                                                                                                       >  1:636414/1‑151 (MQ=255)  tCGTAAACGACCCGGCGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCg                                                                                                                                      >  2:633296/1‑151 (MQ=255)   cGTAAACGACCCGGCGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCg                                                                                                                                      >  1:614262/1‑150 (MQ=255)    gTAAACGACCCGGCGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGaa                                                                                                                                    <  2:36658/151‑1 (MQ=255)        aCGACCCGGCGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTa                                                                                                                                 >  1:292952/1‑150 (MQ=255)          gACCCGGCGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTaac                                                                                                                               >  2:210523/1‑150 (MQ=255)                    tCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATg                                                                                                                     >  2:583919/1‑150 (MQ=255)                     cacaACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCg                                                                                                                   <  1:468745/151‑1 (MQ=255)                      acaACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGt                                                                                                                  >  2:648230/1‑151 (MQ=255)                         aCCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGcc                                                                                                               >  1:487781/1‑151 (MQ=255)                          cccATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCa                                                                                                              <  2:616612/151‑1 (MQ=255)                                aTCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGaa                                                                                                         >  1:479968/1‑150 (MQ=255)                                  cAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAAtt                                                                                                      <  2:81832/151‑1 (MQ=255)                                       cGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCtt                                                                                                  <  1:330952/150‑1 (MQ=255)                                        gagaTTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGt                                                                                                >  1:221421/1‑151 (MQ=255)                                                               aaTCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGt                                                                         <  1:567543/151‑1 (MQ=255)                                                               aaTCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGt                                                                         >  1:200656/1‑151 (MQ=255)                                                               aaTCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGt                                                                         <  1:398748/151‑1 (MQ=255)                                                                 tCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGAGATCAATTTAACGGTTg                                                                       >  2:377987/1‑151 (MQ=255)                                                                          aCAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCa                                                               >  1:312533/1‑150 (MQ=255)                                                                            aGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCaa                                                              >  1:11596/1‑149 (MQ=255)                                                                               tttctttcCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGt                                                         >  1:512399/1‑151 (MQ=255)                                                                               tttctttcCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGt                                                         >  1:31868/1‑151 (MQ=255)                                                                                      cccTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGAc                                                  >  1:350561/1‑151 (MQ=255)                                                                                          tCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTa                                                <  2:474723/149‑1 (MQ=255)                                                                                          tCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAAc                                              >  1:476456/1‑151 (MQ=255)                                                                                             gggCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACt                                             <  2:449457/149‑1 (MQ=255)                                                                                                 cAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTc                                       >  1:427253/1‑151 (MQ=255)                                                                                                     caaatGGCGATGGCCGCGCTGGAGGTTGTCTTAACAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCgcgc                                   <  2:452562/147‑1 (MQ=255)                                                                                                           gCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCttt                             >  2:635403/1‑151 (MQ=255)                                                                                                             gATGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGc                           >  2:678228/1‑151 (MQ=255)                                                                                                              aTGGCCGCGCTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGcc                          <  1:282288/151‑1 (MQ=255)                                                                                                                       cTGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTc                 >  2:468299/1‑151 (MQ=255)                                                                                                                        tGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTct                >  1:642014/1‑151 (MQ=255)                                                                                                                        tGGAGGTTGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTct                >  1:292390/1‑151 (MQ=255)                                                                                                                              ttGTCTTACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTggg          >  1:344629/1‑151 (MQ=255)                                                                                                                                    tACCAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTg     <  2:136799/150‑1 (MQ=255)                                                                                                                                       cAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATc  >  1:448612/1‑150 (MQ=255)                                                                                                                                       cAACACCTCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATc  >  1:459977/1‑150 (MQ=255)                                                                                                                                              |                                                                                                                                               ATCGTAAACGACCCGGCGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATC  >  NC_000913/1225185‑1225468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑