Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,281,664	C→T	R601C (CGT→TGT)	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,281,664	0	C	T	100.0%	179.2 / NA	47	R601C (CGT→TGT)	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/25);  total (22/25)

ATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACTTGATCGACTTTAACGTCCG  >  NC_000913/1281518‑1281809                                                                                                                                                   |                                                                                                                                                  atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTcc                                                                                                                                               <  2:395802/151‑1 (MQ=255)  tatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCg                                                                                                                                              >  1:216304/1‑151 (MQ=255)   atGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCgg                                                                                                                                             >  2:349217/1‑151 (MQ=255)         tATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGt                                                                                                                                        >  1:609795/1‑150 (MQ=255)          aTCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTc                                                                                                                                       >  1:239819/1‑150 (MQ=255)          aTCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTc                                                                                                                                       <  1:491736/150‑1 (MQ=255)          aTCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTc                                                                                                                                       >  2:101648/1‑150 (MQ=255)            cGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTcaca                                                                                                                                    <  2:264330/151‑1 (MQ=255)               ggTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATg                                                                                                                                  <  1:606492/150‑1 (MQ=255)               ggTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGa                                                                                                                                 <  1:455294/151‑1 (MQ=255)                 tCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAAc                                                                                                                               >  1:243665/1‑151 (MQ=255)                         aCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCActtc                                                                                                                       <  2:136470/151‑1 (MQ=255)                             gCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACCGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTAt                                                                                                                   <  2:150131/151‑1 (MQ=255)                                gATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTAttt                                                                                                                 <  1:217791/150‑1 (MQ=255)                                 aTTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCt                                                                                                               >  1:171066/1‑151 (MQ=255)                                 aTTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCt                                                                                                               >  2:447521/1‑151 (MQ=255)                                             tgtgTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTcc                                                                                                    >  1:566121/1‑150 (MQ=255)                                              gtgtCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTcc                                                                                                    >  1:143175/1‑149 (MQ=255)                                                  cGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcc                                                                                                >  1:214697/1‑149 (MQ=255)                                                    gTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTg                                                                                            >  1:453948/1‑151 (MQ=255)                                                     tCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTgg                                                                                           >  1:545786/1‑151 (MQ=255)                                                      cAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTgg                                                                                           <  2:679490/150‑1 (MQ=255)                                                       agagCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCg                                                                                         >  1:153601/1‑151 (MQ=255)                                                            gggggCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAAGCGGCGGGCAGCCGCTGGCGTGTGCCCTTGAATGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTAt                                                                                     >  2:301386/1‑150 (MQ=255)                                                                  ggcTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCg                                                                              <  1:3227/151‑1 (MQ=255)                                                                          gcACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggcg                                                                      <  1:223384/151‑1 (MQ=255)                                                                            aCTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGa                                                                    <  1:296731/151‑1 (MQ=255)                                                                              tATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGa                                                                    >  1:27119/1‑149 (MQ=255)                                                                               aTGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGaa                                                                   <  2:50244/149‑1 (MQ=255)                                                                               aTGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAaga                                                                 <  1:135763/151‑1 (MQ=255)                                                                               aTGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAag                                                                  <  2:517071/150‑1 (MQ=255)                                                                                 gTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGt                                                               <  1:668309/151‑1 (MQ=255)                                                                                       cAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTg                                                          <  1:342513/150‑1 (MQ=255)                                                                                             aaaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGa                                                   <  2:565281/151‑1 (MQ=255)                                                                                               aCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATg                                                 >  1:184136/1‑151 (MQ=255)                                                                                                        gCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACaa                                         >  1:567065/1‑150 (MQ=255)                                                                                                          aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATc                                      >  1:414073/1‑151 (MQ=255)                                                                                                             ccGGCTGGCAGCCGCGGGCGTTTGACCCTGACTGGCAGTGTCCGGCGCGTCAGATGAGCAGGGCTTCTTATTTCTATAACCACTCCAGCCAGTGGCGGTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATccc                                    <  2:101436/150‑1 (MQ=255)                                                                                                                        ccGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGac                        >  2:273832/1‑151 (MQ=255)                                                                                                                        ccGCTGGCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGac                        <  1:168317/151‑1 (MQ=255)                                                                                                                             ggCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACt                     <  1:394730/149‑1 (MQ=255)                                                                                                                             ggCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACt                     <  1:57205/149‑1 (MQ=255)                                                                                                                              gCGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACTTg                   >  1:131779/1‑150 (MQ=255)                                                                                                                               cGTTTGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACTTGAt                 <  1:672884/151‑1 (MQ=255)                                                                                                                                   tGCCCTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACTTGATCGAc             >  2:313816/1‑151 (MQ=255)                                                                                                                                     cccTTGACTGGCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACTTGATCGACtt           <  2:408956/151‑1 (MQ=255)                                                                                                                                              ggCAGTGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACTTGATCGACTTTAACGTCCg  <  2:100966/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGCTATACCGGACACTTGATCGACTTTAACGTCCG  >  NC_000913/1281518‑1281809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑