Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,516	A→G	V377A (GTC→GCC)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,516	0	A	G	100.0%	136.9 / NA	39	V377A (GTC→GCC)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/24);  total (15/24)

ACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGC  >  NC_000913/1307368‑1307661                                                                                                                                                     |                                                                                                                                                  aCTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGcc                                                                                                                                                 <  2:51332/151‑1 (MQ=255)   tACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGcc                                                                                                                                                 >  1:197306/1‑149 (MQ=255)    aCGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAg                                                                                                                                               <  2:543108/150‑1 (MQ=255)      gCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCAt                                                                                                                                            <  1:382108/151‑1 (MQ=255)       cATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATc                                                                                                                                           >  1:265400/1‑151 (MQ=255)         tatCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCg                                                                                                                                          >  2:571867/1‑150 (MQ=255)         tatCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCg                                                                                                                                          >  2:147596/1‑150 (MQ=255)                         gCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCgg                                                                                                                          <  1:225237/150‑1 (MQ=255)                          ccAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGaa                                                                                                                        <  2:23146/151‑1 (MQ=255)                          ccAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGaa                                                                                                                        <  1:558927/151‑1 (MQ=255)                               cAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGat                                                                                                                    >  2:61410/1‑150 (MQ=255)                                    ttAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACt                                                                                                              >  2:87133/1‑151 (MQ=255)                                     tAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATAc                                                                                                               <  2:123522/149‑1 (MQ=255)                                     tAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACt                                                                                                              <  1:546969/150‑1 (MQ=255)                                          cGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATc                                                                                                        <  1:147601/151‑1 (MQ=255)                                                   ccAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCAcccc                                                                                                  <  2:414760/148‑1 (MQ=255)                                                          gCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGcgcc                                                                                        <  1:374038/151‑1 (MQ=255)                                                           ctctTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGcgccg                                                                                       >  2:125767/1‑151 (MQ=255)                                                            tctTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGcgccgc                                                                                      >  1:379992/1‑151 (MQ=255)                                                               tGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGccgccg                                                                                    <  2:241643/150‑1 (MQ=255)                                                                ggTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGt                                                                                   <  1:78506/150‑1 (MQ=255)                                                                ggTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGt                                                                                   <  2:237964/150‑1 (MQ=255)                                                                            cccGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGa                                                                      <  2:202595/151‑1 (MQ=255)                                                                                             aaaTTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTgg                                                     <  2:397363/151‑1 (MQ=255)                                                                                                 tttAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGcac                                                  >  1:664184/1‑150 (MQ=255)                                                                                                    aaCCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAg                                              <  2:390422/151‑1 (MQ=255)                                                                                                            cagccagcagTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGt                                      >  2:513199/1‑151 (MQ=255)                                                                                                             agccagcagTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCg                                       <  2:659243/149‑1 (MQ=255)                                                                                                                    agTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGa                              <  1:571884/151‑1 (MQ=255)                                                                                                                     gTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAt                             <  1:32210/151‑1 (MQ=255)                                                                                                                           tAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAat                        >  2:334940/1‑150 (MQ=255)                                                                                                                                  tGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCAcgc                >  2:433507/1‑151 (MQ=255)                                                                                                                                   gcgcGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCAcgcg               <  1:654346/151‑1 (MQ=255)                                                                                                                                      cgACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCg            <  1:99062/151‑1 (MQ=255)                                                                                                                                           ggCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATaa       <  1:181155/151‑1 (MQ=255)                                                                                                                                            gCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATa        >  2:159541/1‑149 (MQ=255)                                                                                                                                              ccAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGcc    <  2:363063/151‑1 (MQ=255)                                                                                                                                                aGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGc  >  1:26479/1‑151 (MQ=255)                                                                                                                                                  ccGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGc  >  1:128759/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGC  >  NC_000913/1307368‑1307661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑