Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,099	C→T	G183R (GGG→AGG)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,099	0	C	T	100.0%	128.4 / NA	36	G183R (GGG→AGG)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

TATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATT  >  NC_000913/1307952‑1308244                                                                                                                                                    |                                                                                                                                                  tATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCtgg                                                                                                                                                 <  2:207133/150‑1 (MQ=255)       tcatgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTc                                                                                                                                          >  1:95921/1‑151 (MQ=255)        catgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTc                                                                                                                                          <  2:601611/150‑1 (MQ=255)        catgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTc                                                                                                                                          >  1:601629/1‑150 (MQ=255)         atgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTc                                                                                                                                          <  2:156711/149‑1 (MQ=255)             tcatcTTGCGATGTTGGCGCAGGTCCATACAGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCa                                                                                                                                     >  1:478674/1‑150 (MQ=255)                     cGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGc                                                                                                                            >  1:533300/1‑151 (MQ=255)                            ggCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCaatgaat                                                                                                                     <  1:615030/151‑1 (MQ=255)                             gcgcAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCaatgaatg                                                                                                                    <  1:159546/151‑1 (MQ=255)                                         aCGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCa                                                                                                        <  1:100068/151‑1 (MQ=255)                                          cGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCgcgc                                                                                                         >  1:661749/1‑149 (MQ=255)                                                aaaaaCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAgccgcc                                                                                                  <  2:177044/150‑1 (MQ=255)                                                aaaaaCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAgccgcc                                                                                                  >  1:573202/1‑150 (MQ=255)                                                aaaaaCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCa                                                                                                 <  2:360230/151‑1 (MQ=255)                                                 aaaaCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCAt                                                                                                <  1:361040/151‑1 (MQ=255)                                                                         ttAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCGCGGGCCACGGGCGGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAACCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTcc                                                                          <  2:309638/149‑1 (MQ=255)                                                                            aGGCTTCGACCACTTCAATCCCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACCCTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCAt                                                                     <  1:145557/151‑1 (MQ=255)                                                                            aGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCAt                                                                     >  1:346388/1‑151 (MQ=255)                                                                                 tCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATgccgc                                                                >  1:310561/1‑151 (MQ=255)                                                                                  cGACCCCTTCAATACCGGCATTACGCATTAGCGCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGGGCATCAACCGGCAATGACTGCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATgccgcc                                                               <  2:670687/151‑1 (MQ=255)                                                                                   gACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGccgccg                                                              >  2:523678/1‑151 (MQ=255)                                                                                                 cGGCATTACGCATTAGCCCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGAtata                                                <  2:248101/151‑1 (MQ=255)                                                                                                     aTTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAac                                            >  1:62023/1‑151 (MQ=255)                                                                                                     aTTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAac                                            >  2:164564/1‑151 (MQ=255)                                                                                                      ttACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAac                                            >  2:157749/1‑150 (MQ=255)                                                                                                          gCATTGGCTCGAGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATaaaa                                             <  2:128755/145‑1 (MQ=255)                                                                                                          gCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCAt                                       >  1:605085/1‑151 (MQ=255)                                                                                                           cATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCAtc                                      >  2:483401/1‑151 (MQ=255)                                                                                                            aTTAGCTCGGTCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCAtct                                     >  1:332539/1‑151 (MQ=255)                                                                                                                        cACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATg                           <  2:62534/149‑1 (MQ=255)                                                                                                                         aCGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgc                         >  2:294226/1‑150 (MQ=255)                                                                                                                           ggccTGCGGAAAAAAGCCACACCCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgcgc                       <  2:29523/147‑1 (MQ=255)                                                                                                                             gCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGcgcgcg                    >  2:502045/1‑151 (MQ=255)                                                                                                                                     aaaaaGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGAt            <  1:147708/151‑1 (MQ=255)                                                                                                                                       aaaGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGt          <  1:223722/151‑1 (MQ=255)                                                                                                                                       aaaGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGt          >  1:99742/1‑151 (MQ=255)                                                                                                                                                cTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGAtt  <  1:241500/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATT  >  NC_000913/1307952‑1308244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑