Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,324,641	A→G	T182A (ACA→GCA)	yciO →	putative RNA binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,324,641	0	A	G	100.0%	138.4 / NA	40	T182A (ACA→GCA)	yciO	putative RNA binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/20);  total (20/20)

TTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCA  >  NC_000913/1324495‑1324784                                                                                                                                                   |                                                                                                                                                ttGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAAc                                                                                                                                             >  1:205911/1‑151 (MQ=255)     aGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGtt                                                                                                                                         <  2:664454/151‑1 (MQ=255)          tGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTg                                                                                                                                     <  1:68474/150‑1 (MQ=255)           gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGAt                                                                                                                                   <  1:35846/151‑1 (MQ=255)                aaCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTAc                                                                                                                              >  1:394718/1‑151 (MQ=255)                 aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTAcc                                                                                                                             <  1:304177/151‑1 (MQ=255)                 aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCCCGGGCAGAAACCGGCAACGGCTATTGATCTTaac                                                                                                                             >  2:361253/1‑149 (MQ=255)                          ttCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATa                                                                                                                      <  2:71990/149‑1 (MQ=255)                             cACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCg                                                                                                                 >  1:359771/1‑151 (MQ=255)                              aCTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCgg                                                                                                                >  1:34152/1‑151 (MQ=255)                                   gCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCgtg                                                                                                           >  2:139551/1‑151 (MQ=255)                                      aaTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCgtgg                                                                                                          <  2:514918/149‑1 (MQ=255)                                      aaTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGGTATTGATCTTACCGATGATACGCCGGTCgtggt                                                                                                         >  1:27045/1‑150 (MQ=255)                                       aTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGc                                                                                                       <  2:327870/151‑1 (MQ=255)                                         gctgcCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCg                                                                                                      <  2:510311/150‑1 (MQ=255)                                               aGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAg                                                                                                 >  1:620914/1‑149 (MQ=255)                                                        aTTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAt                                                                                      >  1:285534/1‑151 (MQ=255)                                                          ttACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtg                                                                                     <  1:129482/150‑1 (MQ=255)                                                              cGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAg                                                                                <  1:291895/151‑1 (MQ=255)                                                                 aTCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCt                                                                             >  2:298624/1‑151 (MQ=255)                                                                    ggACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCttt                                                                           >  2:643857/1‑150 (MQ=255)                                                                     gACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCt                                                                         >  2:598092/1‑151 (MQ=255)                                                                     gACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCt                                                                         <  2:659884/151‑1 (MQ=255)                                                                         cGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTtata                                                                     <  1:300763/151‑1 (MQ=255)                                                                                aaTTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAAtt                                                              <  1:460069/151‑1 (MQ=255)                                                                                  ttAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGc                                                            >  2:396627/1‑151 (MQ=255)                                                                                           gTCTGGAAAAACAGGTAGATTTGATTATTCGCGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTTCCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACg                                                    <  2:504696/150‑1 (MQ=255)                                                                                             cTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGAt                                                 >  2:446992/1‑151 (MQ=255)                                                                                                 aaaaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGa                                              >  2:397955/1‑150 (MQ=255)                                                                                                   aaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGa                                              >  2:343540/1‑148 (MQ=255)                                                                                                      cAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGGTGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACt                                         >  2:571/1‑150 (MQ=255)                                                                                                          tAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGc                                    >  1:606599/1‑151 (MQ=255)                                                                                                                  attattCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGt                            <  1:277376/151‑1 (MQ=255)                                                                                                                   ttattCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGtt                           >  2:268279/1‑151 (MQ=255)                                                                                                                                  aTCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTg              <  2:562721/149‑1 (MQ=255)                                                                                                                                       gggCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGa       <  1:157414/151‑1 (MQ=255)                                                                                                                                       gggCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGa       <  1:194777/151‑1 (MQ=255)                                                                                                                                          cAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAg    <  2:2247/151‑1 (MQ=255)                                                                                                                                            gAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGc   >  2:123225/1‑150 (MQ=255)                                                                                                                                            gAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCa  <  2:32886/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                TTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCA  >  NC_000913/1324495‑1324784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑