Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	232,838	C→T	G373E (GGA→GAA)	mltD ←	putative membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	232,838	0	C	T	100.0%	197.4 / NA	49	G373E (GGA→GAA)	mltD	putative membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/26);  total (23/26)

GTTCACGCCGTGGCGTTTAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCA  >  NC_000913/232696‑232981                                                                                                                                               |                                                                                                                                                gTTCACGCCGTGGCGTTTAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAg                                                                                                                                          <  1:33940/150‑1 (MQ=255)       gCCGTGGCGTTTAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTc                                                                                                                                   >  2:43489/1‑151 (MQ=255)       gCCGTGGCGTTTAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTc                                                                                                                                   >  2:572439/1‑151 (MQ=255)        ccGTGGCGTTTAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTcc                                                                                                                                  <  1:500082/151‑1 (MQ=255)               gTTTAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCActgct                                                                                                                            >  2:409980/1‑150 (MQ=255)                tttAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACtgctgc                                                                                                                          >  2:338393/1‑151 (MQ=255)                  tAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCa                                                                                                                         <  1:42143/150‑1 (MQ=255)                         gCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTg                                                                                                                 >  1:95198/1‑151 (MQ=255)                           ttGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGt                                                                                                               >  2:326314/1‑151 (MQ=255)                             gAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGc                                                                                                             >  1:19577/1‑151 (MQ=255)                              aaaGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCt                                                                                                            >  1:28304/1‑151 (MQ=255)                              aaaGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCt                                                                                                            >  1:69693/1‑151 (MQ=255)                               aaGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCtt                                                                                                           <  2:627895/151‑1 (MQ=255)                                 gCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTAc                                                                                                         <  2:672496/151‑1 (MQ=255)                                  cGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACg                                                                                                        >  1:297976/1‑151 (MQ=255)                                  cGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACg                                                                                                        <  2:288068/151‑1 (MQ=255)                                  cGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACg                                                                                                        <  2:143210/151‑1 (MQ=255)                                  cGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACg                                                                                                        <  1:566591/151‑1 (MQ=255)                                     aTCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCg                                                                                                     >  2:137765/1‑151 (MQ=255)                                         ccTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGAc                                                                                                 >  1:160977/1‑151 (MQ=255)                                                gcACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGc                                                                                          >  2:129311/1‑151 (MQ=255)                                                gcACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGc                                                                                          <  1:583983/151‑1 (MQ=255)                                                  acacGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCg                                                                                         >  2:548002/1‑150 (MQ=255)                                                   cacGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGAt                                                                                       >  2:119831/1‑151 (MQ=255)                                                    acGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATa                                                                                      <  2:17919/151‑1 (MQ=255)                                                      gATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACt                                                                                    <  1:530070/151‑1 (MQ=255)                                                       aTACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACtt                                                                                   >  2:168428/1‑151 (MQ=255)                                                         aCGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTg                                                                                  >  2:501134/1‑150 (MQ=255)                                                         aCGCAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTg                                                                                  <  2:377207/150‑1 (MQ=255)                                                           gTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGaa                                                                                >  2:465763/1‑150 (MQ=255)                                                            tAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGa                                                                                 >  2:47031/1‑148 (MQ=255)                                                             aaTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAg                                                                              <  2:485563/150‑1 (MQ=255)                                                             aaTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGc                                                                             >  2:361995/1‑151 (MQ=255)                                                              aTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCg                                                                            >  2:230222/1‑151 (MQ=255)                                                                         gttgttTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCaga                                                                  <  2:593709/150‑1 (MQ=255)                                                                           tgttTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGc                                                                <  1:675538/150‑1 (MQ=255)                                                                           tgttTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGc                                                                <  2:547186/150‑1 (MQ=255)                                                                            gttTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGt                                                              <  1:548018/151‑1 (MQ=255)                                                                                                 aCTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTa                                         <  2:265645/151‑1 (MQ=255)                                                                                                 aCTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTa                                         <  1:355293/151‑1 (MQ=255)                                                                                                           ccAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTAt                               <  2:621957/151‑1 (MQ=255)                                                                                                             aaTCGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTg                             >  2:90149/1‑151 (MQ=255)                                                                                                                cGTCAAACTTTGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCg                          <  1:101511/151‑1 (MQ=255)                                                                                                                        tttGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAAcc                    >  2:495630/1‑149 (MQ=255)                                                                                                                          tGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACg                                                                                                <  2:337328/71‑1 (MQ=255)                                                                                                                          tGGCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGc                 <  1:178075/150‑1 (MQ=255)                                                                                                                           ggCCTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGt               <  2:561681/151‑1 (MQ=255)                                                                                                                             ccTGGCTTCAGCTTAGATTCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCg             <  2:290444/151‑1 (MQ=255)                                                                                                                                          tAGATTCGCGCAGTTTGTTCCACTGCTTCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTAcagca  <  1:519285/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                GTTCACGCCGTGGCGTTTAGCAATGCTTGAAAGCGAATCGCCTTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCA  >  NC_000913/232696‑232981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑