Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,190	A→G	V333A (GTG→GCG)	puuP ←	putrescine importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,190	0	A	G	100.0%	167.7 / NA	48	V333A (GTG→GCG)	puuP	putrescine importer
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/28);  total (20/28)

AACACCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCG  >  NC_000913/1358043‑1358333                                                                                                                                                    |                                                                                                                                                aaCACCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCAt                                                                                                                                              <  2:630503/151‑1 (MQ=255)    acCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCTGCCATTTTGGGTGCGCATAGc                                                                                                                                           >  2:150345/1‑151 (MQ=255)    acCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGc                                                                                                                                           <  2:88620/151‑1 (MQ=255)     ccGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGcc                                                                                                                                          <  2:555661/151‑1 (MQ=255)      cGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGcc                                                                                                                                          >  1:464534/1‑150 (MQ=255)       gACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAAAAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGGCCGCCATTTTGGGTGCGCATAGCCa                                                                                                                                         >  2:636338/1‑150 (MQ=255)          aGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAaca                                                                                                                                     <  1:362407/151‑1 (MQ=255)                  gAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTcc                                                                                                                             <  1:427195/151‑1 (MQ=255)                         aaCGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAgca                                                                                                                      <  2:523256/151‑1 (MQ=255)                           cGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAAcaca                                                                                                                    <  2:426351/151‑1 (MQ=255)                                ccaGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGt                                                                                                                <  2:271056/150‑1 (MQ=255)                                   gagCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACg                                                                                                            <  2:339732/151‑1 (MQ=255)                                   gagCACCGAAGTTAATTAACGCTGTGGCGGCAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATAACGTTTAGTGCCGGAGTACGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTc                                                                                                               >  1:372703/1‑148 (MQ=255)                                    agCACCGAAGTTAATTAACGCTGTGGCGGTAACCAAATCGAAGAACCGCGCCGACAACGCCACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCCCCCTTTTGGGGGCGCCTAGCCAAACACGCGCCCCGGAAACACAATGTCAAGc                                                                                                           >  2:67153/1‑151 (MQ=255)                                       aCCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGcccc                                                                                                        >  2:340673/1‑151 (MQ=255)                                         cGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCAt                                                                                                      <  1:326746/151‑1 (MQ=255)                                          gAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATc                                                                                                     <  1:219792/151‑1 (MQ=255)                                           aaGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATc                                                                                                     >  1:567835/1‑150 (MQ=255)                                            aGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATcac                                                                                                   >  1:65799/1‑151 (MQ=255)                                             gTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATcaca                                                                                                  <  1:658633/151‑1 (MQ=255)                                                      cGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGa                                                                                         <  1:330950/151‑1 (MQ=255)                                                      cGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGa                                                                                         <  2:413808/151‑1 (MQ=255)                                                       gCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGAc                                                                                        <  1:122390/151‑1 (MQ=255)                                                         tgtgGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGGGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATAca                                                                                              >  1:557808/1‑143 (MQ=255)                                                               ggTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACg                                                                                >  1:427043/1‑151 (MQ=255)                                                                        aTCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCAtgt                                                                       <  1:323876/151‑1 (MQ=255)                                                                           gaagaaCAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGaa                                                                    <  1:518095/151‑1 (MQ=255)                                                                              gaaCAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgcc                                                                 <  1:45350/151‑1 (MQ=255)                                                                                     gcCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgccaggccag                                                          <  2:57938/151‑1 (MQ=255)                                                                                       cGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgcccggc                                                             >  2:519801/1‑146 (MQ=255)                                                                                        gACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAgg                                                       >  2:373269/1‑151 (MQ=255)                                                                                          cAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGc                                                      >  2:349296/1‑150 (MQ=255)                                                                                           aaCGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCt                                                     >  2:383806/1‑150 (MQ=255)                                                                                                 aCAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACgtgt                                              <  1:406446/151‑1 (MQ=255)                                                                                                           ccATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACg                                     >  2:244452/1‑150 (MQ=255)                                                                                                               aaTGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACgtggtg                                <  1:125796/151‑1 (MQ=255)                                                                                                               aaTGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACgtggtg                                <  2:572491/151‑1 (MQ=255)                                                                                                                  gACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGc                               >  1:76398/1‑149 (MQ=255)                                                                                                                   aCGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGc                               >  1:51751/1‑148 (MQ=255)                                                                                                                       tGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGa                         <  1:140490/150‑1 (MQ=255)                                                                                                                        gAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaaa                       <  1:68960/151‑1 (MQ=255)                                                                                                                         aGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaaa                       >  2:649217/1‑150 (MQ=255)                                                                                                                            gCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCg                   <  1:71476/151‑1 (MQ=255)                                                                                                                              cGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGAc                 <  1:370790/151‑1 (MQ=255)                                                                                                                                     cGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGa             >  2:448542/1‑148 (MQ=255)                                                                                                                                     cGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAaga          <  2:59359/151‑1 (MQ=255)                                                                                                                                       ccATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGGGCAGAGGAAAATCGACTGGAAGAGc        >  2:231721/1‑151 (MQ=255)                                                                                                                                              gggTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTgccg  <  2:377328/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                AACACCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCG  >  NC_000913/1358043‑1358333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑