Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,785	A→G	Q280R (CAG→CGG)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,785	0	A	G	97.7%	156.1 / ‑3.5	44	Q280R (CAG→CGG)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (22/21);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCAT  >  NC_000913/1382644‑1382930                                                                                                                                              |                                                                                                                                                  ttGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCa                                                                                                                                             >  2:600931/1‑148 (MQ=255)          aTCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGATTGGCAGGATCACGACGAAGGCGa                                                                                                                                 <  2:367868/151‑1 (MQ=255)            cGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACa                                                                                                                               <  2:479846/151‑1 (MQ=255)                ggAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGa                                                                                                                           <  2:248228/151‑1 (MQ=255)                 gAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGAt                                                                                                                          <  2:177490/151‑1 (MQ=255)                   gTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATGACCACGAAGGGGGCAggggt                                                                                                                          >  2:291572/1‑145 (MQ=255)                          ggACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGGGATAAAtt                                                                                                                     >  1:599572/1‑147 (MQ=255)                               ggCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATg                                                                                                             <  1:248593/150‑1 (MQ=255)                                                 gTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTTCATTATGCAGGGGTCGGCGGAAAtt                                                                                           >  2:444890/1‑150 (MQ=255)                                                  tGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAAATCCATTATGCAGGGGTCGGCGTAAAtt                                                                                           >  1:650818/1‑149 (MQ=255)                                                   gAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACt                                                                                        <  1:587856/151‑1 (MQ=255)                                                    aaGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTc                                                                                       <  2:329606/151‑1 (MQ=255)                                                    aaGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTc                                                                                       <  1:145783/151‑1 (MQ=255)                                                      ggCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGt                                                                                     <  2:678176/151‑1 (MQ=255)                                                           tGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCAAGAAGAAGGGGAACGGGGAAAATTTCATTTTTAACGGGTCCGCGGAAATTAATCCTTCt                                                                                  >  2:185249/1‑149 (MQ=255)                                                            gATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGa                                                                                <  2:647627/150‑1 (MQ=255)                                                               tttAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCAAGACGAAGGCGACAGGGATAAAATTCATTATTCAGGGGTCGGCGTAAATTACTCGTTCTGATaa                                                                             >  2:193066/1‑150 (MQ=255)                                                                 tAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGGGGCAGTGATAAAATCCATTATGCAGGTGTCGGGGTAAATTACTCGTTCt                                                                                  >  1:139503/1‑143 (MQ=255)                                                                  aaCCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATgg                                                                          <  2:415181/150‑1 (MQ=255)                                                                         tAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATTAACACGACGGCGACAGTGGCAAATTTCATTATGCAGGTGTCTGCGGCAATTATTTGCTCTGATAAATGGCTCAAtt                                                                  >  2:200269/1‑151 (MQ=255)                                                                           ggTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGc                                                                <  2:298176/151‑1 (MQ=255)                                                                              ttATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGAGGGCTACGAAGGAGAGAGCGATAAAATTCATTTTGCTGGTGTCGGGGTAAAAAACTCGGTTTGGTAATGGGCTgaaat                                                                  >  2:109141/1‑144 (MQ=255)                                                                                aTTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGGAAATTACTCGTTCTGATAATGGGCTAAATTGCCgg                                                             >  2:392079/1‑149 (MQ=255)                                                                                   ttACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGAAAGTGATAAATTTCATTATGTAGGTGTCGGCGGAAATTAATCGTTCTGATAATGGGCTAAATTGCCGGATgcg                                                        >  1:332731/1‑151 (MQ=255)                                                                                    tACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCTCGACGAAAGCGACAGGGATAAATTTCATTATGTTGGGGTGGGCGGAAAATACTTGTTCTGGTAATTGGCTAAAATGCCGGATgcgg                                                       >  2:650319/1‑151 (MQ=255)                                                                                     aCGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATgcggc                                                      <  2:70157/151‑1 (MQ=255)                                                                                     aCGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATgcggc                                                      <  1:22324/151‑1 (MQ=255)                                                                                        gTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGcgcg                                                   <  1:314098/151‑1 (MQ=255)                                                                                           aTGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAg                                                 <  1:95465/150‑1 (MQ=255)                                                                                             gATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTAc                                              <  2:416688/151‑1 (MQ=255)                                                                                              aTTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACt                                             <  1:488866/151‑1 (MQ=255)                                                                                                ttCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAAGCGACAGGGATAAAATTCATTATGCAGGGGTCGGCGTAAATTAATCGTTCTGATAATGGGCTAAATTTCCGGATGCGGCGCGAGTACttt                                           >  1:555532/1‑151 (MQ=255)                                                                                                  ccAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGGGATAAAATTCATTATGCAGGGGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGGGTACTTTAt                                         >  1:291810/1‑151 (MQ=255)                                                                                                    aGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATcc                                       <  1:411260/151‑1 (MQ=255)                                                                                                    aGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGGGATAAATTCCCTTATACAGGGGTGGGCGTAAATTACTCGTTCTGATAATGGGGTAAAATTCCCGATGCGGCGCGGGTACtttttac                                       >  2:435849/1‑147 (MQ=255)                                                                                                    aGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGGGATAAATTCCATTATGCAGGTGTCGGCGTAAAATACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGGGTACttttttc                                       >  2:607730/1‑147 (MQ=255)                                                                                                               tCCGTTTCGCTGGAATACGCGTTTGAGTGGCGGGGTCACGACGCAAGCGACAGTGATAAATTCCATTATTCAGGTGTCGGCGGAAAATACTCGGTCTGATAATGGGCTAAATTTCCGGATGGGGGGCGAGGACTTTTTCCGATCTATaaa                             >  1:644732/1‑150 (MQ=255)                                                                                                                 cGTTTCGCTGGAATACGCGTTTGAGTGGCGGGGTTACGACGAAGGCGACAGTGATAAATTTC‑TTTTGCAGGGGTCGGCGGAAATTAATCGTTCTCATAATGGGCTAAATTTTCGGATGCGGCGCGAGTACTTTATCCGATCTTTaaaag                           >  1:355234/1‑147 (MQ=255)                                                                                                                  gTTTCGCTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGGGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGt                          >  1:52460/1‑150 (MQ=255)                                                                                                                        cTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGGCCGTGATAAATTCCATTATTCAGGGGTCGGCGTAAAATAATCGGTCTGATAATGGGGTAAAATTCCGGATGCGGCGCGGGTACTTTTTCCGATCTATAAATGTAGGcc                     >  2:38905/1‑149 (MQ=255)                                                                                                                        cTGGAATACGCGTTTGAGTGGCGGGATCACGACGAAAGCGACCGGGATAAAATCCCTTTTTCAGGGGTCGGCGTAAAATACTCGTTCTTATAATTGGCTAAATTTCCGGGTGCGGCGCGAGTACTTTATTCGATCTATAAATGTAGGCCg                    >  2:64440/1‑150 (MQ=255)                                                                                                                           gAATACGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATa                <  2:524991/151‑1 (MQ=255)                                                                                                                               aCGCGTTTGAGTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGAt            <  2:3819/151‑1 (MQ=255)                                                                                                                                         gTGGCGGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAgcat  <  1:312620/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  TTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCAT  >  NC_000913/1382644‑1382930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑