Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,383,697 C→T A89T (GCC→ACC)  ycjW ← LacI family putative transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,383,6970CT93.6% 143.9 / NA 48A89T (GCC→ACC) ycjWLacI family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (24/20);  minor base G (0/3);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGG  >  NC_000913/1383552‑1383843
                                                                                                                                                 |                                                                                                                                                  
aaaaaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGgtggtg                                                                                                                                                >  2:283156/1‑150 (MQ=255)
 aaaaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGAt                                                                                                                                              >  2:598627/1‑151 (MQ=255)
   aaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGATTTGGTGGTGATgg                                                                                                                                            <  2:272820/151‑1 (MQ=255)
      gACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCa                                                                                                                                          <  1:283161/150‑1 (MQ=255)
      gACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCa                                                                                                                                          <  1:419009/150‑1 (MQ=255)
          cATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAAATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTTGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCa                                                                                                                                      >  2:133573/1‑150 (MQ=255)
             ccGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAAc                                                                                                                                  <  2:342646/151‑1 (MQ=255)
               gCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACtt                                                                                                                                <  2:557301/151‑1 (MQ=255)
                   gCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCaga                                                                                                                            <  2:144404/151‑1 (MQ=255)
                    cGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCag                                                                                                                             >  1:584411/1‑149 (MQ=255)
                          gAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGaaaa                                                                                                                       <  2:178361/149‑1 (MQ=255)
                            gCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACgg                                                                                                                   <  1:584995/151‑1 (MQ=255)
                            gCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACgg                                                                                                                   >  2:212454/1‑151 (MQ=255)
                                  taatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTAcc                                                                                                             >  2:524431/1‑151 (MQ=255)
                                   aatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTAccc                                                                                                            >  1:233023/1‑151 (MQ=255)
                                       ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTCCAGCTTTGGTGGTGATGGCATGCAAACCTTCAGAGAAAAACGGATTTCccgccg                                                                                                        <  2:501588/151‑1 (MQ=255)
                                         tttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCGGCCGt                                                                                                       >  2:334948/1‑150 (MQ=255)
                                         tttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGt                                                                                                       >  1:123841/1‑150 (MQ=255)
                                              tCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGtttt                                                                                                 >  1:538442/1‑151 (MQ=255)
                                              tCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGtttt                                                                                                 >  2:426933/1‑151 (MQ=255)
                                                     cTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGa                                                                                           >  1:386504/1‑150 (MQ=255)
                                                     cTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGa                                                                                           >  1:37474/1‑150 (MQ=255)
                                                       gCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGCCGTTTTTAATATCACGCCGAAACCTTCTTCTTCAGTTTTGTTGGTGATGGAAGGCAAACCTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAAc                                                                                         <  1:157777/150‑1 (MQ=255)
                                                           atttttGTTTTCCCATTTTTGCAGATCTTCTGCCGGTTTGTGCGACTTTTGAAATATCACGTCGAACCCTTCTTTTTCAGTTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGTTTACCCGCCGTAGTTTTGGTCGAACggg                                                                                      <  1:311503/147‑1 (MQ=255)
                                                           aaTTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGt                                                                                     >  1:130934/1‑150 (MQ=255)
                                                             ttttGTTTTCGCATTTTTGCAGTTCTTCTGCCGGATTGTGCGACGTTTGTAATATCCCGTCGAACCCTTCTTTTTCAGCTTTGGTGGTAATGGCATGCAAACCTCCAGAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGt                                                                                     <  2:439567/148‑1 (MQ=255)
                                                             ttttGCTTCCGCTTTTTTGCAGATCTTCTGCGGGATTGTGCGACGTTTGTAATATCACGTCAAACCCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTGCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGt                                                                                     <  1:623388/148‑1 (MQ=255)
                                                                     tCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc                                                                           >  1:25382/1‑150 (MQ=255)
                                                                            tttGCAGATCTTCTGCCGGATTGTGCGGCGTTTGTAATATCACGTCAAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAAAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATgg                                                                    <  1:269229/150‑1 (MQ=255)
                                                                                 aGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCa                                                               >  2:299414/1‑150 (MQ=255)
                                                                                   aTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaa                                                             >  1:257025/1‑150 (MQ=255)
                                                                                    tcttctGCCGGATTGTGCGACGTTTGTAATATCCCGTCAAACCTTTCTTCTTCATTTTTGGTGGTGATGGCATGCAAACCTTCAGAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaaa                                                            <  1:228823/150‑1 (MQ=255)
                                                                                     cttctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAcc                                                          >  2:94571/1‑151 (MQ=255)
                                                                                       tctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCg                                                        >  2:346354/1‑151 (MQ=255)
                                                                                         tGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGa                                                       >  1:276285/1‑150 (MQ=255)
                                                                                                ttGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTc                                                >  2:545270/1‑150 (MQ=255)
                                                                                                   tgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAgcg                                            >  2:70082/1‑151 (MQ=255)
                                                                                                      gagttttGTAATATCACGCCGAACCCTTTTTCTCCAGC‑‑TGGTGGTGATGGCATGCAAACCTTCAAAAAAAACCGTTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGcgcgcg                                        <  1:101857/146‑1 (MQ=255)
                                                                                                        cGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCgg                                       >  2:590749/1‑151 (MQ=255)
                                                                                                                atatCCCGTCAAACCCTTCTTTTTAAGCTTGGGTGGTGATGGAATGAAAACCTTAAGAAAAAACCGTTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCt                                <  2:562190/150‑1 (MQ=255)
                                                                                                                    cccGTCGAACCCTTCTTCTTCAGTTTGGGGGGTGATGGCATGCAAAACTTAAAAAAAAACCGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGttt                           <  2:177210/149‑1 (MQ=255)
                                                                                                                    cACGTCAAACCCTTCTTCTTCAGTTTTGGTGGTGAGGGCATGCAAAACTTCAGAGAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTg                              <  1:439695/148‑1 (MQ=255)
                                                                                                                     acggcaaacccTTCTTCTTCAGCTTTGGTGGTGATGGCATGAAAACCTTCAAAGAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGttt                           <  1:317530/141‑1 (MQ=255)
                                                                                                                      cGTCGAAACCTTCTTCTTCAGCTTTGGGGGTGATGGCATGAAAACCTCCAGAGAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTg                          <  2:141024/150‑1 (MQ=255)
                                                                                                                       gccaaaaCCTTTTTCTCCAGCTTGGGGGGTGATGGCATGCAAAACTTCAGAAAAAACGGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTg                          <  1:245271/145‑1 (MQ=255)
                                                                                                                           aaaCCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGAt                    >  1:357280/1‑151 (MQ=255)
                                                                                                                                       tcatttttGGTGGTGTGGTCATGCAAAACTTAAGAAAAAAAGGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAAtt           <  2:649132/143‑1 (MQ=255)
                                                                                                                                          gCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCa      >  1:492541/1‑150 (MQ=255)
                                                                                                                                             ttGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATgg  >  2:301380/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGG  >  NC_000913/1383552‑1383843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑