Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,448,822	T→C	V435A (GTC→GCC)	feaB →	phenylacetaldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,448,822	0	T	C	100.0%	131.4 / NA	35	V435A (GTC→GCC)	feaB	phenylacetaldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/17);  total (18/17)

GCCAACGCTGGTGGTAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCG  >  NC_000913/1448676‑1448968                                                                                                                                                   |                                                                                                                                                   gcCAACGCTGGTGGTAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTgg                                                                                                                                                <  1:459511/151‑1 (MQ=255)   cAACGCTGGTGGTAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGAc                                                                                                                                              <  1:330546/151‑1 (MQ=255)      cGCTGGTGGTAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCa                                                                                                                                           <  1:443826/151‑1 (MQ=255)          ggtggtAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAAt                                                                                                                                       <  2:191370/151‑1 (MQ=255)           gtggtAAATCCCGAAGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAAtc                                                                                                                                      <  2:56763/151‑1 (MQ=255)             ggtAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAAtctc                                                                                                                                    >  1:641121/1‑151 (MQ=255)               tAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAAtctctc                                                                                                                                  >  2:248643/1‑151 (MQ=255)               tAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAAtctct                                                                                                                                   <  2:300681/150‑1 (MQ=255)                           cTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTgg                                                                                                                       >  1:21836/1‑150 (MQ=255)                             aaaTTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGaa                                                                                                                     <  1:666752/150‑1 (MQ=255)                               aTTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAatat                                                                                                                  <  2:634276/151‑1 (MQ=255)                                       tAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCg                                                                                                          >  1:510854/1‑151 (MQ=255)                                            cGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTa                                                                                                      <  2:413977/150‑1 (MQ=255)                                                         ttGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACgg                                                                                         >  1:547346/1‑150 (MQ=255)                                                          tGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGgtg                                                                                       >  2:610230/1‑151 (MQ=255)                                                           ggTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGgtg                                                                                       <  2:100850/150‑1 (MQ=255)                                                            gtacGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTAAAGGCAGGGACGGtgtg                                                                                     >  2:272641/4‑151 (MQ=255)                                                              ccGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTgg                                                                                    <  1:602416/150‑1 (MQ=255)                                                                 gtggtAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTaa                                                                                <  1:395127/151‑1 (MQ=255)                                                                    gtAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAAc                                                                              >  1:288874/1‑150 (MQ=255)                                                                     tAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAAc                                                                              >  2:605370/1‑149 (MQ=255)                                                                          cTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCAt                                                                        >  2:121762/1‑150 (MQ=255)                                                                            ggTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATAcc                                                                     <  1:597120/151‑1 (MQ=255)                                                                                cGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTa                                                                  >  1:269602/1‑150 (MQ=255)                                                                                 gAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCCGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGGGTGGGAAAACAGCCATACCTTaa                                                                 >  2:52421/1‑150 (MQ=255)                                                                                     aGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGAc                                                            >  1:99058/1‑151 (MQ=255)                                                                                       cGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGc                                                          >  1:655182/1‑151 (MQ=255)                                                                                         gATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCt                                                         >  2:475938/1‑150 (MQ=255)                                                                                                       gTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTtgg                                           >  1:287907/1‑150 (MQ=255)                                                                                                         tACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACGGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTtggtg                                         <  2:35929/150‑1 (MQ=255)                                                                                                          aCAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGGGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTggg                                       >  1:251827/1‑151 (MQ=255)                                                                                                                  cAAACGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCa                               <  1:153753/151‑1 (MQ=255)                                                                                                                   aaaCGACACGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAg                              <  1:364576/151‑1 (MQ=255)                                                                                                                           cGGAATATGGCTTAACTGCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTAGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGAAGTCAGGaa                       >  2:226369/1‑150 (MQ=255)                                                                                                                                            gCCAGTGCCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGcc     <  2:553289/151‑1 (MQ=255)                                                                                                                                                gtgcCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCg  >  1:154197/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GCCAACGCTGGTGGTAAATCCCGATGCTAAATTGCGCTTAACTCGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCGTTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCG  >  NC_000913/1448676‑1448968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑