Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,449,092	A→G	L753P (CTG→CCG)	tynA ←	tyramine oxidase, copper‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,449,092	0	A	G	100.0%	122.6 / NA	34	L753P (CTG→CCG)	tynA	tyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/17);  total (17/17)

ACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGT  >  NC_000913/1448947‑1449233                                                                                                                                                   |                                                                                                                                              aCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGcc                                                                                                                                          <  1:9357/151‑1 (MQ=255)     gCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTa                                                                                                                                      <  1:220380/151‑1 (MQ=255)       cGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGc                                                                                                                                    <  2:467019/151‑1 (MQ=255)               tGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGt                                                                                                                            >  1:202675/1‑151 (MQ=255)                      gACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCa                                                                                                                     <  2:363989/151‑1 (MQ=255)                          ggctggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAaaga                                                                                                                 <  2:386653/151‑1 (MQ=255)                          ggctggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAaag                                                                                                                  <  1:108725/150‑1 (MQ=255)                              ggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGtt                                                                                                             <  1:613045/151‑1 (MQ=255)                                   gTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATg                                                                                                        <  2:541366/151‑1 (MQ=255)                                      ggTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGt                                                                                                      <  2:271361/150‑1 (MQ=255)                                       gtgtGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGttt                                                                                                    <  1:549034/151‑1 (MQ=255)                                           gAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcag                                                                                                 <  2:518224/150‑1 (MQ=255)                                                      ggTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTAc                                                                                     >  1:464685/1‑151 (MQ=255)                                                                       aaTCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGCCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGAACCCATTCGGTCGGCAt                                                                      >  1:73564/1‑150 (MQ=255)                                                                            ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCgg                                                                <  2:377397/150‑1 (MQ=255)                                                                            ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGc                                                               >  1:392628/1‑151 (MQ=255)                                                                                   tCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCtt                                                        >  1:234534/1‑151 (MQ=255)                                                                                      tAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCgg                                                     >  1:576953/1‑151 (MQ=255)                                                                                       aaGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGgc                                                    <  1:390852/151‑1 (MQ=255)                                                                                        aGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGgcg                                                   <  1:212296/151‑1 (MQ=255)                                                                                          tAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGgcgc                                                  >  2:669068/1‑150 (MQ=255)                                                                                           aaaaaaCGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCgg                                                >  1:579392/1‑151 (MQ=255)                                                                                               aaCGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCca                                            <  2:3165/151‑1 (MQ=255)                                                                                                 cGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCcac                                           >  1:169794/1‑150 (MQ=255)                                                                                                                   tttttGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCggtgg                          >  2:151828/1‑150 (MQ=255)                                                                                                                     tttGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTc                        >  1:17745/1‑150 (MQ=255)                                                                                                                      ttGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGAGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCAt                      >  2:554042/1‑151 (MQ=255)                                                                                                                             aaaCAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGAc                >  2:214042/1‑150 (MQ=255)                                                                                                                                cAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACg            <  1:248648/151‑1 (MQ=255)                                                                                                                                 aaTCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACgg           >  2:541938/1‑151 (MQ=255)                                                                                                                                 aaTCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACg            <  2:610748/150‑1 (MQ=255)                                                                                                                                    cACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGt        >  2:643671/1‑151 (MQ=255)                                                                                                                                      cTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCg      >  1:271697/1‑151 (MQ=255)                                                                                                                                          tctttcttCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGgtgt  >  2:477120/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                              ACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGT  >  NC_000913/1448947‑1449233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑