Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,580,435	C→G	G119A (GGC→GCC)	ydeM ←	putative YdeN‑specific sulfatase‑maturating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,580,435	0	C	G	100.0%	173.7 / NA	50	G119A (GGC→GCC)	ydeM	putative YdeN‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (17/33);  total (17/33)

GGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGG  >  NC_000913/1580289‑1580578                                                                                                                                                   |                                                                                                                                                ggTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCatc                                                                                                                                             <  1:490057/151‑1 (MQ=255)          cATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCatcgatcg                                                                                                                                        >  1:561067/1‑147 (MQ=255)          cATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGAt                                                                                                                                    <  1:320850/151‑1 (MQ=255)                    attaATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAg                                                                                                                           <  1:471358/150‑1 (MQ=255)                      taATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGaa                                                                                                                         >  2:602873/1‑150 (MQ=255)                        aTGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAAtt                                                                                                                      <  1:61501/151‑1 (MQ=255)                        aTGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAAtt                                                                                                                      <  2:519137/151‑1 (MQ=255)                         tGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTc                                                                                                                     >  2:123300/1‑151 (MQ=255)                          gACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCa                                                                                                                    <  2:524138/151‑1 (MQ=255)                          gACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCa                                                                                                                    <  1:88386/151‑1 (MQ=255)                              gTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGtt                                                                                                                <  1:152021/151‑1 (MQ=255)                                      aTTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTg                                                                                                           >  1:243755/1‑148 (MQ=255)                                        tAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGaa                                                                                                       <  1:378666/150‑1 (MQ=255)                                        tAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGaa                                                                                                       >  1:260767/1‑150 (MQ=255)                                         aaaCTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGACTTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGaa                                                                                                       >  2:476645/1‑149 (MQ=255)                                                   tGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcatt                                                                                           >  1:489434/1‑151 (MQ=255)                                                   tGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcat                                                                                            <  1:628391/150‑1 (MQ=255)                                                    gATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattc                                                                                          <  1:141508/151‑1 (MQ=255)                                                       atGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattcatt                                                                                       <  1:444642/151‑1 (MQ=255)                                                         gATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCatta                                                                                      <  2:154954/150‑1 (MQ=255)                                                            ttCAGACGCGCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTc                                                                                   <  2:138769/150‑1 (MQ=255)                                                               aGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATa                                                                               <  1:144617/151‑1 (MQ=255)                                                                   gCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAAt                                                                           >  2:423932/1‑151 (MQ=255)                                                                       gATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGcc                                                                        >  2:225061/1‑150 (MQ=255)                                                                       gATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCa                                                                       <  2:65894/151‑1 (MQ=255)                                                                       gATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCa                                                                       <  2:429611/151‑1 (MQ=255)                                                                         tGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCAtt                                                                     >  1:669663/1‑151 (MQ=255)                                                                              gctATCACTTTTGCAAAAGTACCGTTTCCTGAAGTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACTCCATTCATTATTCAAGAAAATGCCATTCGttt                                                                <  2:463787/151‑1 (MQ=255)                                                                              gctATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGt                                                                  <  2:74879/149‑1 (MQ=255)                                                                                 aTCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTa                                                             <  2:582072/151‑1 (MQ=255)                                                                                     cTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATg                                                          <  1:192574/150‑1 (MQ=255)                                                                                     cTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATg                                                          <  1:472817/150‑1 (MQ=255)                                                                                        ttGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCAtt                                                      >  2:16125/1‑151 (MQ=255)                                                                                         tGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCAtt                                                      <  2:605605/150‑1 (MQ=255)                                                                                          gCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTa                                                     >  1:394298/1‑150 (MQ=255)                                                                                            aaaaGTACCGTTGCCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTaaaa                                                  <  2:90925/151‑1 (MQ=255)                                                                                                gTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATAc                                              <  1:557300/151‑1 (MQ=255)                                                                                                    cGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGttt                                          <  2:448868/151‑1 (MQ=255)                                                                                                        aCCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTg                                       <  1:77087/150‑1 (MQ=255)                                                                                                          cTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGcc                                    >  1:141415/1‑151 (MQ=255)                                                                                                            gAATTATTGCGGCGGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAAAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTgg                                      <  2:280922/147‑1 (MQ=255)                                                                                                                    gCGTCTGTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGc                            <  2:4002/149‑1 (MQ=255)                                                                                                                    gCGTCTGTAACGGTCATGTAACTCCTGGGGGGCAGCGATCGAGATACCAACCAGAAATTCATGTTCTTTTAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTCATGCATTAAAAATCCGTTTTTGGCCTGCATAGCg                           <  2:68289/150‑1 (MQ=255)                                                                                                                          gTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttgtt                      <  2:392503/149‑1 (MQ=255)                                                                                                                          gTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttgtt                      <  1:498619/149‑1 (MQ=255)                                                                                                                          gTAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGa                    <  1:369230/151‑1 (MQ=255)                                                                                                                           tAACGGTCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGAt                   >  1:543481/1‑151 (MQ=255)                                                                                                                                 tCATGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGa              >  1:272073/1‑150 (MQ=255)                                                                                                                                    tGTAACTCCTGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATa           >  1:67313/1‑150 (MQ=255)                                                                                                                                             tGGGGGGCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACgg  >  2:489214/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                GGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGG  >  NC_000913/1580289‑1580578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑