Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,595,660	A→G	pseudogene (2328/3861 nt)	yneO ←	pseudogene, AidA homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,595,660	0	A	G	100.0%	137.8 / NA	37	pseudogene (2328/3861 nt)	yneO	pseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/14);  total (23/14)

GCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCGTGTAATCTCCT  >  NC_000913/1595514‑1595804                                                                                                                                                   |                                                                                                                                                 gcagGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGa                                                                                                                                              <  1:218818/151‑1 (MQ=255)         atCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACt                                                                                                                                       <  1:116405/150‑1 (MQ=255)                   aaaaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGtt                                                                                                                            >  2:303447/1‑151 (MQ=255)                   aaaaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGtt                                                                                                                            >  1:159296/1‑151 (MQ=255)                   aaaaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGtt                                                                                                                            >  1:224221/1‑151 (MQ=255)                     aaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTgg                                                                                                                          >  1:82067/1‑151 (MQ=255)                      atgatgGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTggg                                                                                                                         >  1:591531/1‑151 (MQ=255)                         atgGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCaa                                                                                                                      <  1:127904/151‑1 (MQ=255)                             tACCTCCATTCATGGCCAGTCCAACAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAacac                                                                                                                   >  1:302974/1‑150 (MQ=255)                                  ccATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCg                                                                                                              <  2:282942/150‑1 (MQ=255)                                  ccATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCg                                                                                                              >  1:539504/1‑150 (MQ=255)                                       cATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTc                                                                                                         >  2:24533/1‑150 (MQ=255)                                        aTGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTc                                                                                                         >  2:344021/1‑149 (MQ=255)                                          ggCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGt                                                                                                      <  1:661463/150‑1 (MQ=255)                                           gCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTcc                                                                                                    <  1:492457/151‑1 (MQ=255)                                             cAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCt                                                                                                  >  2:263392/1‑151 (MQ=255)                                               gTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGt                                                                                                >  1:353068/1‑151 (MQ=255)                                                     caATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCTCGGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCAt                                                                                           <  2:593542/150‑1 (MQ=255)                                                         ggATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTa                                                                                      <  1:602034/151‑1 (MQ=255)                                                               ctctCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGcc                                                                                 >  2:677161/1‑150 (MQ=255)                                                                 ctcCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCaa                                                                               >  2:49914/1‑150 (MQ=255)                                                                     aaCTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATc                                                                          >  1:614385/1‑151 (MQ=255)                                                                         tttACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGt                                                                      >  1:635111/1‑151 (MQ=255)                                                                           tACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTc                                                                     <  1:14959/150‑1 (MQ=255)                                                                                         tttCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTg                                                      >  1:599009/1‑151 (MQ=255)                                                                                             aCTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACt                                                  >  2:273204/1‑151 (MQ=255)                                                                                                     aCTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCaa                                          <  2:596375/151‑1 (MQ=255)                                                                                                          aaCATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCAt                                     >  1:443149/1‑151 (MQ=255)                                                                                                                   tGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTg                             >  1:198914/1‑150 (MQ=255)                                                                                                                        aaGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAgcgc                        <  2:535272/150‑1 (MQ=255)                                                                                                                          gcgcAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCAtt                     <  2:167079/151‑1 (MQ=255)                                                                                                                             cAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGt                  >  1:153253/1‑151 (MQ=255)                                                                                                                              cgCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTc                 >  2:414314/2‑151 (MQ=255)                                                                                                                                  gtgtTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCg             >  2:69202/1‑151 (MQ=255)                                                                                                                                      tgtCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCgtgt          >  2:343021/1‑150 (MQ=255)                                                                                                                                       gtCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCGTGTaa        <  2:655949/151‑1 (MQ=255)                                                                                                                                              tCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCGTGTAATCTCCt  <  2:377030/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCGTGTAATCTCCT  >  NC_000913/1595514‑1595804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑