Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,784	A→G	S356S (AGT→AGC)	uidC ←	putative outer membrane porin for beta‑glucuronides porin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,784	0	A	G	100.0%	121.3 / NA	33	S356S (AGT→AGC)	uidC	putative outer membrane porin for beta‑glucuronides porin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

TCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTACTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCC  >  NC_000913/1691636‑1691931                                                                                                                                                     |                                                                                                                                                    tCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCt                                                                                                                                                   >  1:558718/1‑151 (MQ=255)        aTCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTc                                                                                                                                             >  1:71182/1‑150 (MQ=255)          cGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCAt                                                                                                                                           <  1:572019/150‑1 (MQ=255)          cGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATa                                                                                                                                          <  1:612662/151‑1 (MQ=255)                       gCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCGCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTcaca                                                                                                                             <  1:227149/151‑1 (MQ=255)                         cATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAAt                                                                                                                           <  2:564246/151‑1 (MQ=255)                              cgcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTg                                                                                                                      >  2:122339/1‑151 (MQ=255)                              cgcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTg                                                                                                                      <  2:441517/151‑1 (MQ=255)                               gcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGc                                                                                                                     >  2:308516/1‑151 (MQ=255)                                     cccGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGt                                                                                                               >  2:530028/1‑151 (MQ=255)                                     cccGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGt                                                                                                               <  2:143052/151‑1 (MQ=255)                                             ttATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTa                                                                                                        >  1:359727/1‑150 (MQ=255)                                                 cGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTg                                                                                                   >  1:375190/1‑151 (MQ=255)                                                   gcagcaAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTgcg                                                                                                 <  1:169975/151‑1 (MQ=255)                                                    cagcaAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCGCCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCTCAATCGGTGCAAATGTCAGCGTAAATTgcg                                                                                                 <  2:66941/150‑1 (MQ=255)                                                        aagaaagaAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGtt                                                                                            >  2:162980/1‑151 (MQ=255)                                                        aagaaagaAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGtt                                                                                            <  1:159968/151‑1 (MQ=255)                                                                aaTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGAt                                                                                     >  1:525230/1‑150 (MQ=255)                                                                 aTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAg                                                                                   <  2:468009/151‑1 (MQ=255)                                                                            gTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGccc                                                                          <  2:576039/149‑1 (MQ=255)                                                                              aaCGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAAtt                                                                      <  2:515052/151‑1 (MQ=255)                                                                                     cTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAgg                                                                >  1:161469/1‑150 (MQ=255)                                                                                        cGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTg                                                            >  1:575921/1‑151 (MQ=255)                                                                                        cGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTg                                                            <  1:123802/151‑1 (MQ=255)                                                                                             aCCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTc                                                       <  2:98241/151‑1 (MQ=255)                                                                                                       tCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGtt                                             >  2:417860/1‑151 (MQ=255)                                                                                                         aTACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTAc                                           <  2:429542/151‑1 (MQ=255)                                                                                                              accTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATc                                       >  1:355344/1‑150 (MQ=255)                                                                                                                   aGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACt                                 >  2:559794/1‑151 (MQ=255)                                                                                                                                 tCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAggg                     <  2:557839/149‑1 (MQ=255)                                                                                                                                             tctcGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCgg       >  2:199173/1‑151 (MQ=255)                                                                                                                                                cGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCg        <  1:530833/147‑1 (MQ=255)                                                                                                                                                  tttGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATcc  >  1:34221/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTACTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCC  >  NC_000913/1691636‑1691931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑