Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,717,165	T→C	T20A (ACG→GCG)	tyrS ←	tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,717,165	0	T	C	100.0%	95.6 / NA	29	T20A (ACG→GCG)	tyrS	tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/17);  total (12/17)

TGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAAT  >  NC_000913/1717024‑1717311                                                                                                                                              |                                                                                                                                                   tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTgg                                                                                                                                             <  1:660265/149‑1 (MQ=255) tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTgg                                                                                                                                             <  1:448888/149‑1 (MQ=255)      cgcGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGTTGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCACGTCCGCCACCTGGGCTACCa                                                                                                                                      <  2:158157/149‑1 (MQ=255)             gctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGcccc                                                                                                                                 <  2:177714/149‑1 (MQ=255)               tgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGc                                                                                                                              >  1:117406/1‑150 (MQ=255)               tgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGACGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGct                                                                                                                             >  2:530877/1‑151 (MQ=255)                            ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGttt                                                                                                                >  1:247636/1‑151 (MQ=255)                                 aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                                            >  1:342142/1‑150 (MQ=255)                                       ggAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTa                                                                                                      <  2:606860/150‑1 (MQ=255)                                       ggAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAc                                                                                                     <  1:437480/151‑1 (MQ=255)                                             aGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTg                                                                                               <  2:60562/151‑1 (MQ=255)                                                         tGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtct                                                                                   >  1:20397/1‑151 (MQ=255)                                                                   cAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtata                                                                          <  1:350164/150‑1 (MQ=255)                                                                       ggTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACg                                                                     >  1:122103/1‑151 (MQ=255)                                                                          aGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                                                  <  1:666325/151‑1 (MQ=255)                                                                                         aTAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTa                                                   <  1:445989/151‑1 (MQ=255)                                                                                                gcgATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACt                                            >  2:166050/1‑151 (MQ=255)                                                                                                   aTCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACttt                                          <  2:176033/150‑1 (MQ=255)                                                                                                        gCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCGCCTTTGCCGGTACACGACTTTTCGCCa                                    <  2:187866/151‑1 (MQ=255)                                                                                                        gCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGcc                                     >  1:673418/1‑150 (MQ=255)                                                                                                        gCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCa                                    <  1:495812/151‑1 (MQ=255)                                                                                                          cTTGCGCCAGTCGCTCTGCTAACGCTCCCTCGTCCGCCACCTGGCCTACCAGCCCCCGCTCTTGCAATCGTTTATCCAAGTCACTGCTTGCTATCAATATCTCCATGTATATAACTATTGTACCTATGCCGGTCCACGACTTTTCCCCa                                    >  2:366532/1‑149 (MQ=255)                                                                                                            tGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGa                                  <  1:591993/149‑1 (MQ=255)                                                                                                              cgcCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCg                              >  1:184579/1‑151 (MQ=255)                                                                                                                     cGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAagag                        <  2:435579/150‑1 (MQ=255)                                                                                                                      gCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGAc                      <  1:171153/151‑1 (MQ=255)                                                                                                                          tGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCACAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACAt                    >  2:363781/1‑149 (MQ=255)                                                                                                                               aCGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATaa             <  2:273771/151‑1 (MQ=255)                                                                                                                                   ttCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTg         >  2:607467/1‑151 (MQ=255)                                                                                                                                          tccgccACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAAt  >  2:471026/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                   TGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAAT  >  NC_000913/1717024‑1717311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑