Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,222	C→T	P50L (CCG→CTG)	ydiK →	UPF0118 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,222	0	C	T	100.0%	112.3 / NA	32	P50L (CCG→CTG)	ydiK	UPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

ATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCC  >  NC_000913/1769074‑1769357                                                                                                                                                     |                                                                                                                                        aTGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACctggctgg                                                                                                                                       >  1:608632/1‑151 (MQ=255)  tGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACctggctgg                                                                                                                                       >  1:618445/1‑150 (MQ=255)      aaaTGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGGTTGTTCAACCCTTTATTCTCGGCTTTGCCTGGGTCGGTAAGGTGGTTATCGCCACCTGGCTGGGAtt                                                                                                                                   >  1:578375/1‑150 (MQ=255)        aTGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGtt                                                                                                                                >  2:261673/1‑151 (MQ=255)        aTGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGtt                                                                                                                                <  1:621946/151‑1 (MQ=255)                cAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTTTTCAACCCCTTATTTTCGGCTTTGCATTGGCCCGTCCGGTGGTTATCTCCACCTTGGTGGGATTGTTTCGgtt                                                                                                                          >  2:583309/1‑149 (MQ=255)                  gCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCa                                                                                                                       >  1:674250/1‑150 (MQ=255)                     cAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCaaaa                                                                                                                    >  2:505781/1‑150 (MQ=255)                      aGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCaaa                                                                                                                     >  1:488933/1‑148 (MQ=255)                      aGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGa                                                                                                                  <  2:578618/151‑1 (MQ=255)                            gTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGt                                                                                                            <  1:351237/151‑1 (MQ=255)                                  caAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTTGCTGGTCTTGTTACGTTTGCAAAAGATCATGTTTGgcc                                                                                                      >  1:604056/1‑151 (MQ=255)                                  caAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGgcc                                                                                                      <  1:345475/151‑1 (MQ=255)                                  caAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGgc                                                                                                       <  2:209829/150‑1 (MQ=255)                                       tCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGccgccg                                                                                                  <  1:128535/150‑1 (MQ=255)                                                   gCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGTTACGGTGGTTATCGCCCCCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGtt                                                                                     <  2:41387/151‑1 (MQ=255)                                                                 tcatGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGc                                                                        >  2:650323/1‑150 (MQ=255)                                                                                gTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATgg                                                         >  1:277087/1‑150 (MQ=255)                                                                                    gtgGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGgtg                                                       <  1:358878/148‑1 (MQ=255)                                                                                        aTTGTTCAACCCTTTATTCTCGGTTTTGCATGGTCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTTCTTTTGCAAAAGATCATGTTTGGTCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTatc                                                 <  2:353042/150‑1 (MQ=255)                                                                                               aaCCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTAt                                         <  1:588008/151‑1 (MQ=255)                                                                                                 cccTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTAttg                                       <  1:322771/151‑1 (MQ=255)                                                                                                         tctcGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTg                               >  2:53435/1‑151 (MQ=255)                                                                                                                   gCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTa                     <  1:135779/151‑1 (MQ=255)                                                                                                                   gCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTa                     <  2:426906/151‑1 (MQ=255)                                                                                                                     aTGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTAtc                   >  1:290517/1‑151 (MQ=255)                                                                                                                       gggCTGCTACGTTGGTTATCGCCACCTGGCTCGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTAtc                   <  2:356002/149‑1 (MQ=255)                                                                                                                         gCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTAtcgtcg               <  1:650342/151‑1 (MQ=255)                                                                                                                            ggTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACg            >  1:416347/1‑151 (MQ=255)                                                                                                                              tACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACg            >  1:312576/1‑149 (MQ=255)                                                                                                                                  gtggtTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGc       <  2:342050/150‑1 (MQ=255)                                                                                                                                     gtTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGcc   <  1:311066/151‑1 (MQ=255)                                                                                                                                     gtTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGc    <  2:359155/150‑1 (MQ=255)                                                                                                                                       tATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGccc  <  2:402448/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                        ATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCC  >  NC_000913/1769074‑1769357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑