Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,803,248	T→C	pseudogene (155/426 nt)	arpB →	pseudogene, ankyrin repeats

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,803,248	0	T	C	100.0%	116.0 / NA	33	pseudogene (155/426 nt)	arpB	pseudogene, ankyrin repeats
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/18);  total (15/18)

CAAAACGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCTGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTCATTACATTACACTATAAAAAAGAA  >  NC_000913/1803100‑1803393                                                                                                                                                     |                                                                                                                                                  cAAAACGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGt                                                                                                                                                 <  2:678432/151‑1 (MQ=255)    aaCGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCAt                                                                                                                                              <  1:23618/151‑1 (MQ=255)     aCGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCGCCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATa                                                                                                                                             <  1:426896/151‑1 (MQ=255)            cATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGtt                                                                                                                                      <  2:31902/151‑1 (MQ=255)                aTCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACt                                                                                                                                   <  1:80266/150‑1 (MQ=255)                  cAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAg                                                                                                                                <  1:588343/151‑1 (MQ=255)                     aaCTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGaa                                                                                                                             <  1:362810/151‑1 (MQ=255)                       cTCATTATAAGTCTCCTCATAGATTGCACATCGATAGGGACATAACAACACCTTAATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAAtt                                                                                                                           >  2:630163/1‑151 (MQ=255)                          aTTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTa                                                                                                                         <  2:249179/150‑1 (MQ=255)                            tataAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCg                                                                                                                      >  1:621465/1‑151 (MQ=255)                                aGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCaa                                                                                                                   >  2:657807/1‑150 (MQ=255)                                            gATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGaaaa                                                                                                        <  2:44436/149‑1 (MQ=255)                                               tGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAatat                                                                                                    <  2:543399/150‑1 (MQ=255)                                                gCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATc                                                                                                   <  1:160326/150‑1 (MQ=255)                                                          aGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGtt                                                                                        >  1:326818/1‑151 (MQ=255)                                                           gCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGtt                                                                                        >  2:554169/1‑150 (MQ=255)                                                                         ccTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACCTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCtt                                                                          <  1:321784/150‑1 (MQ=255)                                                                            tcatcaGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCttttt                                                                       >  1:406899/1‑150 (MQ=255)                                                                                caGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCAt                                                                  >  1:673827/1‑151 (MQ=255)                                                                                        aTTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACttt                                                           <  2:619606/150‑1 (MQ=255)                                                                                        aTTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTg                                                          <  2:554346/151‑1 (MQ=255)                                                                                              cAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACtt                                                    <  2:569603/151‑1 (MQ=255)                                                                                                 tttGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCAc                                                 >  2:504776/1‑151 (MQ=255)                                                                                                       cgcCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACccatcc                                           >  1:538271/1‑151 (MQ=255)                                                                                                          cAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACccatccat                                         >  1:19679/1‑150 (MQ=255)                                                                                                            gCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACa                                      <  1:552746/151‑1 (MQ=255)                                                                                                                atcaCCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGaa                                   >  2:569666/1‑150 (MQ=255)                                                                                                                   aCCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAat                                >  2:156303/1‑150 (MQ=255)                                                                                                                       tACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACt                           >  2:244468/1‑151 (MQ=255)                                                                                                                             aTACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTcattac                     <  2:490161/151‑1 (MQ=255)                                                                                                                                ccTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTcattacatt                  >  1:608691/1‑151 (MQ=255)                                                                                                                                          aaGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTCATTACATTACACTATaaa        <  1:470176/151‑1 (MQ=255)                                                                                                                                                 aTGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTCATTACATTACACTATAAAAAAGaa  >  1:491837/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CAAAACGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCTGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTCATTACATTACACTATAAAAAAGAA  >  NC_000913/1803100‑1803393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑