Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,844,948	T→A	intergenic (‑77/+51)	ynjI ← / ← topB	inner membrane protein/DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,844,948	0	T	A	100.0%	183.6 / NA	48	intergenic (‑77/+51)	ynjI/topB	inner membrane protein/DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (27/21);  total (27/21)

ATTAAATATTTCCCAGCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACT  >  NC_000913/1844800‑1845088                                                                                                                                                     |                                                                                                                                             aTTAAATATTTCCCAGCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAAcc                                                                                                                                            >  1:382376/1‑151 (MQ=255)      atatTTCCCAGCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTgg                                                                                                                                       >  1:22493/1‑151 (MQ=255)      atatTTCCCAGCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTgg                                                                                                                                       <  2:460019/151‑1 (MQ=255)        atTTCCCAGCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGat                                                                                                                                     >  1:373670/1‑151 (MQ=255)          ttCCCAGCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGata                                                                                                                                    <  2:314375/150‑1 (MQ=255)            cccAGCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATAtt                                                                                                                                  <  1:402292/150‑1 (MQ=255)                gCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGa                                                                                                                             <  1:505634/151‑1 (MQ=255)                 ccATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGaa                                                                                                                            <  2:666882/151‑1 (MQ=255)                 ccATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGa                                                                                                                             <  2:265277/150‑1 (MQ=255)                  cATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAat                                                                                                                           <  1:205901/151‑1 (MQ=255)                      aaaaGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATg                                                                                                                        >  2:670631/1‑150 (MQ=255)                      aaaaGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATg                                                                                                                        <  2:263525/150‑1 (MQ=255)                       aaaGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGAc                                                                                                                      >  1:667451/1‑151 (MQ=255)                        aaGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACt                                                                                                                     <  1:312050/151‑1 (MQ=255)                                ttCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGc                                                                                                             >  2:288214/1‑151 (MQ=255)                                 tctcGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATa                                                                                                               <  2:383007/148‑1 (MQ=255)                                        cccAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGaaa                                                                                                       <  1:450174/149‑1 (MQ=255)                                        cccAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAg                                                                                                      <  2:277940/150‑1 (MQ=255)                                           aGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTc                                                                                                  >  1:420257/1‑151 (MQ=255)                                                      gTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACg                                                                                       <  2:641997/151‑1 (MQ=255)                                                       taataaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCt                                                                                     >  1:109571/1‑151 (MQ=255)                                                        aataaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCt                                                                                     <  1:671334/151‑1 (MQ=255)                                                                  ttttCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGc                                                                           >  2:193407/1‑151 (MQ=255)                                                                        tcaCATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCg                                                                      >  1:39019/1‑150 (MQ=255)                                                                         cacaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGAc                                                                    >  2:161961/1‑151 (MQ=255)                                                                           caTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACtt                                                                  >  1:481766/1‑151 (MQ=255)                                                                                         aCATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTgg                                                        >  2:570333/1‑147 (MQ=255)                                                                                           aTACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACt                                                  >  1:630995/1‑151 (MQ=255)                                                                                             aCTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACttt                                                >  1:400413/1‑151 (MQ=255)                                                                                               tttAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACttttt                                              >  1:84523/1‑151 (MQ=255)                                                                                                ttAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACtttttt                                             >  1:345053/1‑151 (MQ=255)                                                                                                ttAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACtttt                                               <  1:101903/149‑1 (MQ=255)                                                                                                         aTAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTAc                                    >  2:330467/1‑151 (MQ=255)                                                                                                           aaTTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGt                                  >  2:312352/1‑151 (MQ=255)                                                                                                                 aTCCATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTAcg                             <  2:608922/150‑1 (MQ=255)                                                                                                                    cATGCAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGt                         <  2:310982/151‑1 (MQ=255)                                                                                                                        cAAAAAAAATATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCa                      <  2:423926/150‑1 (MQ=255)                                                                                                                           aaaaaaTATCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCt                  <  1:307000/151‑1 (MQ=255)                                                                                                                                  atCCAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCtt           <  1:470893/151‑1 (MQ=255)                                                                                                                                     cAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATc        >  1:661604/1‑151 (MQ=255)                                                                                                                                     cAACGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATc        >  2:453347/1‑151 (MQ=255)                                                                                                                                      aaCGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATc        >  1:207355/1‑150 (MQ=255)                                                                                                                                      aaCGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATc        >  1:24594/1‑150 (MQ=255)                                                                                                                                      aaCGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCa       <  2:67189/151‑1 (MQ=255)                                                                                                                                       aCGAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATc        >  1:262642/1‑149 (MQ=255)                                                                                                                                         gAATTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGc     >  2:304927/1‑150 (MQ=255)                                                                                                                                          aaTTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCAc   >  2:463263/1‑151 (MQ=255)                                                                                                                                           aTTTTCTTGAACCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACt  >  1:290848/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                             ATTAAATATTTCCCAGCCATTAAAAGAATACTTCTCGCTCCCAGGATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATTCACTCGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACT  >  NC_000913/1844800‑1845088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑