Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,877,132	A→G	H75R (CAC→CGC)	yeaO →	DUF488 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,877,132	0	A	G	100.0%	178.6 / NA	50	H75R (CAC→CGC)	yeaO	DUF488 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/28);  total (22/28)

GGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTT  >  NC_000913/1876985‑1877279                                                                                                                                                    |                                                                                                                                                    ggCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTGTCGCGTGCAATATCTTGCAGAACTGGCGCAACGCGa                                                                                                                                                  <  2:311816/151‑1 (MQ=255)               aaaaaaaCCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGc                                                                                                                                    >  1:193327/1‑151 (MQ=255)                    aaCCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTg                                                                                                                               <  2:82516/151‑1 (MQ=255)                    aaCCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTg                                                                                                                               <  1:480010/151‑1 (MQ=255)                        gATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCgg                                                                                                                           >  1:8617/1‑151 (MQ=255)                         aTTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGa                                                                                                                          <  1:140365/151‑1 (MQ=255)                             aGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATc                                                                                                                      >  1:507586/1‑151 (MQ=255)                              gCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATc                                                                                                                      >  1:70342/1‑150 (MQ=255)                                 cTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGcc                                                                                                                   >  1:129143/1‑150 (MQ=255)                                             gATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGc                                                                                                      >  1:275702/1‑151 (MQ=255)                                               tAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCt                                                                                                     <  2:425828/150‑1 (MQ=255)                                                aaaGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTg                                                                                                    <  1:230554/150‑1 (MQ=255)                                                             cGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTc                                                                                      <  2:202895/151‑1 (MQ=255)                                                                            gcAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAAcaccac                                                                       >  1:257839/1‑151 (MQ=255)                                                                                aGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGc                                                                     >  1:311169/1‑149 (MQ=255)                                                                                aGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGc                                                                     >  1:441461/1‑149 (MQ=255)                                                                                 gCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGc                                                                     >  2:354662/1‑148 (MQ=255)                                                                                  ccTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGaa                                                                 <  2:447572/151‑1 (MQ=255)                                                                                  ccTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGaa                                                                 <  1:356010/151‑1 (MQ=255)                                                                                      tCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACACCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCa                                                              <  2:11237/150‑1 (MQ=255)                                                                                          ggCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATgcgc                                                         >  2:590075/1‑151 (MQ=255)                                                                                             gAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTgg                                                      >  2:666021/1‑151 (MQ=255)                                                                                              aaGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGt                                                     <  1:85102/151‑1 (MQ=255)                                                                                                 tcgtcgATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt                                                  >  2:102124/1‑151 (MQ=255)                                                                                                          aTGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg                                         <  2:495249/151‑1 (MQ=255)                                                                                                          aTGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg                                         >  1:243615/1‑151 (MQ=255)                                                                                                            gCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTgg                                        <  2:220804/150‑1 (MQ=255)                                                                                                            gCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTgg                                        <  1:147095/150‑1 (MQ=255)                                                                                                             cAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt                                      <  2:193322/151‑1 (MQ=255)                                                                                                                 cTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTACTGGCCGACTGGCTACGt                                  >  2:375585/1‑151 (MQ=255)                                                                                                                     cgcgAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCt                              >  2:28204/1‑151 (MQ=255)                                                                                                                     cgcgAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTAATCAGCAAAAACCACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGc                               <  1:328662/150‑1 (MQ=255)                                                                                                                       cgAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtg                            >  1:524168/1‑151 (MQ=255)                                                                                                                        gAGCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtgt                           <  2:34695/151‑1 (MQ=255)                                                                                                                          gCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtgtg                          >  1:353555/1‑150 (MQ=255)                                                                                                                          gCAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGa                         <  2:475781/151‑1 (MQ=255)                                                                                                                           cAATATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAt                        >  1:508495/1‑151 (MQ=255)                                                                                                                            aaTATCTTGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAtt                       >  1:157698/1‑151 (MQ=255)                                                                                                                                   tGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGt                  <  1:250696/149‑1 (MQ=255)                                                                                                                                   tGCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTa                 <  1:443545/150‑1 (MQ=255)                                                                                                                                    gCAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTAc                <  1:559856/150‑1 (MQ=255)                                                                                                                                     cAGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTAc                >  1:397736/1‑149 (MQ=255)                                                                                                                                      aGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGc             <  1:625377/151‑1 (MQ=255)                                                                                                                                      aGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGc             >  2:517314/1‑151 (MQ=255)                                                                                                                                      aGAACTGGCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGc             <  1:333069/151‑1 (MQ=255)                                                                                                                                            ggCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCcgg       <  1:588861/151‑1 (MQ=255)                                                                                                                                            ggCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCcg        <  2:191055/150‑1 (MQ=255)                                                                                                                                            ggCGCAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATCTTAGTACAGCATCcg        <  1:624843/150‑1 (MQ=255)                                                                                                                                               gcAACGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCcggcgg    <  2:362042/151‑1 (MQ=255)                                                                                                                                                 aaCGCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGtt  <  1:377638/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTT  >  NC_000913/1876985‑1877279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑