Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,005	T→C	H177R (CAC→CGC)	proQ ←	RNA chaperone, putative ProP translation regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,005	0	T	C	100.0%	151.8 / NA	43	H177R (CAC→CGC)	proQ	RNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/24);  total (19/24)

CGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAC  >  NC_000913/1914858‑1915149                                                                                                                                                    |                                                                                                                                                 cgcACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCt                                                                                                                                               <  1:335101/151‑1 (MQ=255)    acaATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGTTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGtt                                                                                                                                            <  2:55075/151‑1 (MQ=255)         cAAAGACATACCCGAATGCATCTGGACGCGGACGCCGTCTTTGGTGATTTCTAGTACGGTGGCATACAGCGCGTTTTGACCCGCTTGCACCTTCAGGGCTTGGCCGACAGTGAGAGCTGAAATGTCAGAAACCGGGTTGCGCTGtgctgg                                                                                                                                        <  2:113171/150‑3 (MQ=255)          aaaGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTcgc                                                                                                                                      >  2:447897/1‑151 (MQ=255)                  aCCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGc                                                                                                                               <  2:106610/150‑1 (MQ=255)                   cccGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCtt                                                                                                                             <  2:65108/151‑1 (MQ=255)                   cccGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCtt                                                                                                                             <  2:461717/151‑1 (MQ=255)                   cccGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCt                                                                                                                              <  2:436053/150‑1 (MQ=255)                    ccGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCttt                                                                                                                            <  1:590521/151‑1 (MQ=255)                     cGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCtttt                                                                                                                           >  1:20802/1‑151 (MQ=255)                      gAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTa                                                                                                                          <  2:562589/151‑1 (MQ=255)                        aTTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACt                                                                                                                        <  1:581898/151‑1 (MQ=255)                                ggacgcggacgcCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTgg                                                                                                                >  2:233587/1‑151 (MQ=255)                                  acgcggacgcCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGgcg                                                                                                              >  2:98458/1‑151 (MQ=255)                                   cgcggacgcCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGgcgc                                                                                                             <  1:100905/151‑1 (MQ=255)                                          gcCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTctct                                                                                                      <  1:303073/151‑1 (MQ=255)                                            cGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTAc                                                                                                    <  2:670351/151‑1 (MQ=255)                                             gTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTAcc                                                                                                   <  2:397606/151‑1 (MQ=255)                                             gTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGCTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTAcc                                                                                                   <  1:657987/151‑1 (MQ=255)                                                  tGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCtt                                                                                              >  2:361586/1‑151 (MQ=255)                                                     tGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTg                                                                                           >  2:203849/1‑151 (MQ=255)                                                      gATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTg                                                                                           <  2:310709/150‑1 (MQ=255)                                                         ttCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTgcgc                                                                                        >  2:446696/1‑150 (MQ=255)                                                                      gCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGtt                                                                          <  2:130728/151‑1 (MQ=255)                                                                            atcGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAgcgc                                                                    <  2:586559/151‑1 (MQ=255)                                                                               gcgTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCctt                                                                 >  1:663823/1‑151 (MQ=255)                                                                               gcgTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCctt                                                                 >  2:348162/1‑151 (MQ=255)                                                                                     tGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTg                                                            <  2:461610/150‑1 (MQ=255)                                                                                                     tCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTc                                             <  1:479675/149‑1 (MQ=255)                                                                                                      cAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCgg                                           <  2:255025/150‑1 (MQ=255)                                                                                                      cAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCgg                                           >  2:677876/1‑150 (MQ=255)                                                                                                             tGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCt                                   >  2:251006/1‑151 (MQ=255)                                                                                                                 cGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCg                               >  2:637160/1‑151 (MQ=255)                                                                                                                  gACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGt                              >  2:532173/1‑151 (MQ=255)                                                                                                                     aGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcg                           >  1:297351/1‑151 (MQ=255)                                                                                                                        cAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcg                         <  2:663083/150‑1 (MQ=255)                                                                                                                         agagCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                       <  1:520580/151‑1 (MQ=255)                                                                                                                         agagCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcgg                        >  2:360674/1‑150 (MQ=255)                                                                                                                           agCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                       >  2:497835/1‑149 (MQ=255)                                                                                                                                aaaTGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGc                <  1:528597/151‑1 (MQ=255)                                                                                                                                  aTGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGt              >  2:193542/1‑151 (MQ=255)                                                                                                                                             cGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTtctc    >  2:667895/1‑150 (MQ=255)                                                                                                                                              ggggTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAc  >  2:305239/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAC  >  NC_000913/1914858‑1915149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑