Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,946,069	T→C	intergenic (‑93/+46)	ruvA ← / ← yobI	component of RuvABC resolvasome, regulatory subunit/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,946,069	0	T	C	100.0%	94.1 / NA	27	intergenic (‑93/+46)	ruvA/yobI	component of RuvABC resolvasome, regulatory subunit/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/20);  total (7/20)

TTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATT  >  NC_000913/1945922‑1946203                                                                                                                                                    |                                                                                                                                       ttAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGtc                                                                                                                                     >  2:97878/1‑151 (MQ=255)   aaCACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGtctc                                                                                                                                   >  2:197478/1‑151 (MQ=255)     cacCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGa                                                                                                                                 <  2:605246/151‑1 (MQ=255)       ccAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATg                                                                                                                               <  2:447127/151‑1 (MQ=255)           cGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACt                                                                                                                           <  1:4827/151‑1 (MQ=255)                      tttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGc                                                                                                                 <  1:364752/150‑1 (MQ=255)                      tttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGc                                                                                                                 >  1:505442/1‑150 (MQ=255)                         cAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAg                                                                                                             <  1:671844/151‑1 (MQ=255)                           atgatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGta                                                                                                           <  2:301415/151‑1 (MQ=255)                                 atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAAttt                                                                                                     <  1:306116/151‑1 (MQ=255)                                          aGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                                                                                            <  1:469876/151‑1 (MQ=255)                                          aGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                                                                                            <  2:367832/151‑1 (MQ=255)                                                   aTCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTatat                                                                                     <  2:571984/149‑1 (MQ=255)                                                     cacaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTgg                                                                                  <  1:175564/150‑1 (MQ=255)                                                                ccTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAttt                                                                      <  1:525876/151‑1 (MQ=255)                                                                   gCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAtttt                                                                     <  1:191669/149‑1 (MQ=255)                                                                   gCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATATATTCTACAGGTTATATTGGAAGCAAATATTTTa                                                                    >  1:537364/1‑150 (MQ=255)                                                                                 aGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTc                                                       >  1:568540/1‑149 (MQ=255)                                                                                 aGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAg                                                     <  1:202726/151‑1 (MQ=255)                                                                                     aaTGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTgaa                                                 <  2:531964/151‑1 (MQ=255)                                                                                     aaTGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTga                                                  <  2:212290/150‑1 (MQ=255)                                                                                     aaTGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTga                                                  <  1:274830/150‑1 (MQ=255)                                                                                                          gCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAatat                             >  2:492067/1‑150 (MQ=255)                                                                                                                     atCCAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGcct                  <  1:369846/150‑1 (MQ=255)                                                                                                                        cAGCGAAGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTg              <  2:204995/151‑1 (MQ=255)                                                                                                                              aGGATGAAGAAAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGaaaaa         >  1:292479/1‑150 (MQ=255)                                                                                                                                    aagaaAACTTGCGAGGCGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAAtt  <  2:666436/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                       TTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATT  >  NC_000913/1945922‑1946203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑