Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	283,790	A→G	D197G (GAC→GGC)	yagF →	CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,790	0	A	G	100.0%	179.6 / NA	50	D197G (GAC→GGC)	yagF	CP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/25);  total (25/25)

GCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCG  >  NC_000913/283643‑283936                                                                                                                                                    |                                                                                                                                                   gCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGgcgc                                                                                                                                                 >  1:665073/1‑151 (MQ=255)      cTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGc                                                                                                                                             >  2:160821/1‑150 (MQ=255)         ccGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAgg                                                                                                                                         >  2:511996/1‑151 (MQ=255)          cggcgcgGCGGGCGGTGATCGGCGTAGCGACTTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCa                                                                                                                                            <  2:593630/144‑1 (MQ=255)               cggcggGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGc                                                                                                                                      >  1:550699/1‑148 (MQ=255)               cggcggGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGc                                                                                                                                      >  2:556115/1‑148 (MQ=255)                   gggcggTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGAcct                                                                                                                                <  2:339975/150‑2 (MQ=255)                      cggTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCgg                                                                                                                            >  1:579519/1‑151 (MQ=255)                      cggTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCgg                                                                                                                            >  1:661590/1‑151 (MQ=255)                       ggTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGgc                                                                                                                           <  2:438360/151‑1 (MQ=255)                                cGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTc                                                                                                                  <  2:389920/151‑1 (MQ=255)                                    gCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCa                                                                                                              <  1:115071/151‑1 (MQ=255)                                        ccTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCa                                                                                                          <  1:550002/151‑1 (MQ=255)                                         cTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCAc                                                                                                         >  1:273033/1‑151 (MQ=255)                                         cTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCAc                                                                                                         >  1:163969/1‑151 (MQ=255)                                             gATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAAc                                                                                                     <  1:456042/151‑1 (MQ=255)                                               tAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGAGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAActc                                                                                                   >  1:141575/1‑151 (MQ=255)                                                  aGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGCAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTcc                                                                                                >  2:678533/1‑151 (MQ=255)                                                   gggCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTccc                                                                                               >  1:197114/1‑151 (MQ=255)                                                   gggCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTccc                                                                                               >  1:518036/1‑151 (MQ=255)                                                   gggCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTccc                                                                                               >  1:558033/1‑151 (MQ=255)                                                          ccGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCagga                                                                                        <  1:302617/151‑1 (MQ=255)                                                           cGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCagg                                                                                         >  2:427299/1‑149 (MQ=255)                                                                 caTGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGgccgcc                                                                                 <  1:401248/151‑1 (MQ=255)                                                                    gATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGaa                                                                              <  2:204604/151‑1 (MQ=255)                                                                        gcgcGGGCCGCGATGCGCGAGCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCGAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTgg                                                                          <  2:277879/151‑1 (MQ=255)                                                                               ccGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTcg                                                                   <  2:373338/151‑1 (MQ=255)                                                                                  cgATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCgcgc                                                                >  2:76731/1‑151 (MQ=255)                                                                                    aTGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCgcgcct                                                              <  1:47609/151‑1 (MQ=255)                                                                                    aTGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCgcgcct                                                              <  2:206684/151‑1 (MQ=255)                                                                                      gCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCgcgcctgc                                                            >  1:129902/1‑151 (MQ=255)                                                                                      gCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCgcgcctg                                                             <  1:422837/150‑1 (MQ=255)                                                                                          gACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCgcgcctgcgcct                                                        <  2:191759/151‑1 (MQ=255)                                                                                              tGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGcc                                                    >  1:472377/1‑151 (MQ=255)                                                                                              tGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGcc                                                    >  2:85979/1‑151 (MQ=255)                                                                                                ccGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCg                                                   >  2:674377/1‑150 (MQ=255)                                                                                                 cGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCggg                                                 >  2:87403/1‑151 (MQ=255)                                                                                                  gACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGggc                                                <  1:272215/151‑1 (MQ=255)                                                                                                    cTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGggcgg                                              >  2:454579/1‑151 (MQ=255)                                                                                                       ttCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGcggcgg                                           >  1:61051/1‑151 (MQ=255)                                                                                                          tGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGgtg                                        >  1:44985/1‑151 (MQ=255)                                                                                                           ggTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGgtg                                        <  2:632075/150‑1 (MQ=255)                                                                                                                cgggcgggGCGACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGtt                                  <  1:515777/151‑1 (MQ=255)                                                                                                                       ggcgACGCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGc                           <  1:201210/151‑1 (MQ=255)                                                                                                                             gCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCAcggcg                     >  2:526994/1‑151 (MQ=255)                                                                                                                             gCTGCCGCCGACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCAcggc                      <  1:17562/150‑1 (MQ=255)                                                                                                                                   gccgACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCAcc               <  1:76734/151‑1 (MQ=255)                                                                                                                                     cgACCGTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTc             <  2:5815/151‑1 (MQ=255)                                                                                                                                          gTCGGGGAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAgg        <  2:130614/151‑1 (MQ=255)                                                                                                                                               ggAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTc   <  2:665052/151‑1 (MQ=255)                                                                                                                                                gAAGGCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTcg  >  1:391560/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCG  >  NC_000913/283643‑283936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑