Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,994,904	T→C	E152E (GAA→GAG)	uvrY ←	response regulator in two‑component regulatory system with BarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,994,904	0	T	C	100.0%	158.0 / NA	41	E152E (GAA→GAG)	uvrY	response regulator in two‑component regulatory system with BarA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/28);  total (13/28)

GAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGTTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTCCTGCGGAGCCGCGCCT  >  NC_000913/1994756‑1995048                                                                                                                                                     |                                                                                                                                                 gAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTc                                                                                                                                                >  2:228296/1‑151 (MQ=255)  aGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCa                                                                                                                                               <  1:242225/151‑1 (MQ=255)              aTCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCa                                                                                                                                   <  2:179164/151‑1 (MQ=255)                       aaTGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCtt                                                                                                                          <  1:179289/151‑1 (MQ=255)                         tGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTc                                                                                                                        >  1:612776/1‑151 (MQ=255)                             tagtttaCTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGt                                                                                                                     <  2:205828/150‑1 (MQ=255)                               gtttaCTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGtttt                                                                                                                  <  1:433121/151‑1 (MQ=255)                                         cATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCg                                                                                                        >  1:443608/1‑151 (MQ=255)                                           tACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGAt                                                                                                      >  1:436461/1‑151 (MQ=255)                                           tACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGAt                                                                                                      >  1:573017/1‑151 (MQ=255)                                            aCGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATc                                                                                                     >  2:71515/1‑151 (MQ=255)                                               aTAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTgg                                                                                                  >  2:311582/1‑151 (MQ=255)                                                 aGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCt                                                                                                >  1:479884/1‑151 (MQ=255)                                                  gCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCtt                                                                                               <  2:578898/151‑1 (MQ=255)                                                   cGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCtt                                                                                               >  1:139121/1‑150 (MQ=255)                                                     gTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAAc                                                                                            >  1:580399/1‑151 (MQ=255)                                                               aCCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATttgtt                                                                                  >  2:378485/1‑151 (MQ=255)                                                                 cGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGa                                                                                <  1:260719/151‑1 (MQ=255)                                                                             gagaTTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGa                                                                     <  2:345175/150‑1 (MQ=255)                                                                                aTTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCa                                                                 <  2:470634/151‑1 (MQ=255)                                                                                    aGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGt                                                             <  1:311589/151‑1 (MQ=255)                                                                                        gTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAAc                                                          <  2:510024/150‑1 (MQ=255)                                                                                           cTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTg                                                      >  2:504434/1‑151 (MQ=255)                                                                                                aTCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTg                                                 >  1:509573/1‑151 (MQ=255)                                                                                                    cATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGaa                                               <  2:667043/149‑1 (MQ=255)                                                                                                    cATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAAt                                              <  2:660384/150‑1 (MQ=255)                                                                                                       tGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGa                                           <  1:58681/150‑1 (MQ=255)                                                                                                        gACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGAc                                          <  1:548695/150‑1 (MQ=255)                                                                                                        gACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACa                                         <  1:68812/151‑1 (MQ=255)                                                                                                             tCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGaa                                      <  2:418758/149‑1 (MQ=255)                                                                                                             tCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGaa                                      <  1:468855/149‑1 (MQ=255)                                                                                                             tCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAAc                                     <  2:41392/150‑1 (MQ=255)                                                                                                                ggCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACg                                    <  1:324897/148‑1 (MQ=255)                                                                                                                ggCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGa                                   <  2:172087/149‑1 (MQ=255)                                                                                                                ggCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAAt                                 <  2:501690/151‑1 (MQ=255)                                                                                                                   ccTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCg                               <  1:532560/150‑1 (MQ=255)                                                                                                                       ggTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACt                           <  1:659738/150‑1 (MQ=255)                                                                                                                              atcaGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACtt                   <  2:40658/151‑1 (MQ=255)                                                                                                                                caGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTc                  <  2:296301/150‑1 (MQ=255)                                                                                                                                            cAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTCCTGCGGAGCcgcg     <  2:400984/151‑1 (MQ=255)                                                                                                                                                tCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTCCTGCGGAGCCGCGCCt  <  1:65769/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGTTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTCCTGCGGAGCCGCGCCT  >  NC_000913/1994756‑1995048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑