Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,088,373	G→A	G252G (GGC→GGT)	yeeZ ←	putative epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,088,373	0	G	A	100.0%	180.1 / NA	47	G252G (GGC→GGT)	yeeZ	putative epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/27);  total (20/27)

GGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTGCCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGCCTTTAG  >  NC_000913/2088228‑2088518                                                                                                                                                  |                                                                                                                                                  ggATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATc                                                                                                                                              <  1:325360/151‑1 (MQ=255)    tcatcaACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCaat                                                                                                                                           >  2:664371/1‑151 (MQ=255)     catcaACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCaata                                                                                                                                          <  1:589906/151‑1 (MQ=255)     catcaACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCaat                                                                                                                                           >  2:627218/1‑150 (MQ=255)       tcaACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCaataat                                                                                                                                        >  2:572678/1‑151 (MQ=255)         aaCACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCaata                                                                                                                                          <  1:394021/147‑1 (MQ=255)         aaCACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCt                                                                                                                                      >  2:175883/1‑151 (MQ=255)         aaCACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCt                                                                                                                                      <  1:268874/151‑1 (MQ=255)           cacaTCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTa                                                                                                                                    <  1:214060/151‑1 (MQ=255)              aTCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCt                                                                                                                                 <  1:670742/151‑1 (MQ=255)                 cagcagTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTg                                                                                                                              <  1:673739/151‑1 (MQ=255)                           ttCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTcc                                                                                                                     >  2:222480/1‑150 (MQ=255)                            tCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTcc                                                                                                                     >  2:344624/1‑149 (MQ=255)                              aTACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGAc                                                                                                                 >  1:478028/1‑151 (MQ=255)                                 cacGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTAt                                                                                                              <  2:66282/151‑1 (MQ=255)                                   cGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTAttt                                                                                                            <  1:238699/151‑1 (MQ=255)                                    gCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTc                                                                                                           >  1:175241/1‑151 (MQ=255)                                       cccTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACGAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTCCCTTTGCCGCTGTCCAGACTATTTCt                                                                                                          <  1:93267/149‑1 (MQ=255)                                                 gCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAgg                                                                                              <  1:674743/151‑1 (MQ=255)                                                             ctgctgGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGcccc                                                                                    >  1:58416/1‑149 (MQ=255)                                                               gctgGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTa                                                                                <  2:468880/151‑1 (MQ=255)                                                                  ggCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTaaa                                                                              <  1:102240/150‑1 (MQ=255)                                                                   gCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACg                                                                            <  1:499546/151‑1 (MQ=255)                                                                    cGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACgg                                                                           >  1:560295/1‑151 (MQ=255)                                                                                   cAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAg                                                            <  1:608146/151‑1 (MQ=255)                                                                                     gCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGaa                                                          <  1:451422/151‑1 (MQ=255)                                                                                              ccAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTAcg                                                 >  2:168031/1‑151 (MQ=255)                                                                                              ccAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTAcg                                                 <  2:560278/151‑1 (MQ=255)                                                                                               cAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTAcgc                                                >  2:271553/1‑151 (MQ=255)                                                                                                aGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTAcgcg                                               <  1:430832/151‑1 (MQ=255)                                                                                                  cGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACgcgc                                              >  1:383415/1‑150 (MQ=255)                                                                                                   ggATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACgcgc                                              >  1:641557/1‑149 (MQ=255)                                                                                                   ggATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAg                                            <  2:391339/151‑1 (MQ=255)                                                                                                             aCTGGTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACTCGCAGGGTGAGCt                                    <  1:280893/149‑1 (MQ=255)                                                                                                                ggTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGgcg                                <  1:269070/150‑1 (MQ=255)                                                                                                                ggTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGgc                                 <  2:184294/149‑1 (MQ=255)                                                                                                                 gTATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGgcgc                               >  1:291551/1‑150 (MQ=255)                                                                                                                  tATTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAGAACATTACGCGCAGGGTGAGCTGGCGcac                             >  1:193772/1‑151 (MQ=255)                                                                                                                   aTTCAAATCCCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGcaca                            <  2:143933/151‑1 (MQ=255)                                                                                                                       aaaTCTCAGTTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTTCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACat                           >  2:461527/1‑148 (MQ=255)                                                                                                                       aaaTCCCAATTCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACatata                        <  1:79462/151‑1 (MQ=255)                                                                                                                             cAGTTCATTACAAATCCGACTACCATCAATAAACTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAg                  <  2:448234/151‑1 (MQ=255)                                                                                                                                 tCATTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGAtg               <  2:532269/150‑1 (MQ=255)                                                                                                                                   aTTACAAATCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATgtgt            >  2:235255/1‑151 (MQ=255)                                                                                                                                         aaTCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGCCt      >  2:198226/1‑151 (MQ=255)                                                                                                                                          aTCCGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGCCtt     >  1:112298/1‑151 (MQ=255)                                                                                                                                             cGACTACCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGCCTTTAg  <  1:549520/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTTTACTCCAGCGGCATTACCAGCGGATCGGGATACTGGTATTCAAATCCCAGTTCATTACAAATCCGACTGCCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGCCTTTAG  >  NC_000913/2088228‑2088518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑