Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,129,612	G→A	L13L (CTC→CTT)	wcaE ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,129,612	0	G	A	100.0%	192.0 / NA	50	L13L (CTC→CTT)	wcaE	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/31);  total (19/31)

AAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGC  >  NC_000913/2129465‑2129754                                                                                                                                                    |                                                                                                                                               aaaGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAgg                                                                                                                                             >  1:125371/1‑151 (MQ=255)   aGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGtt                                                                                                                                           <  1:467964/151‑1 (MQ=255)     cGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTAc                                                                                                                                         <  2:451686/151‑1 (MQ=255)     cGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTAc                                                                                                                                         <  1:292730/151‑1 (MQ=255)        aGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGaa                                                                                                                                      <  1:493838/151‑1 (MQ=255)        aGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGa                                                                                                                                       >  1:324498/1‑150 (MQ=255)          gTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTTCCGTCGTTGGAACCGCCATCGACAACAATCCATTCTAAGCTGATATCTTCCTCCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGaa                                                                                                                                      <  1:147913/149‑1 (MQ=255)          gTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAAc                                                                                                                                    <  1:513867/151‑1 (MQ=255)                 aTACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATGGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGAc                                                                                                                               >  2:645461/1‑149 (MQ=255)                 aTACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGAc                                                                                                                               >  1:326500/1‑149 (MQ=255)                        tGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTAt                                                                                                                      >  1:355092/1‑151 (MQ=255)                          agaTTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGc                                                                                                                    >  2:179093/1‑151 (MQ=255)                             ttttCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTa                                                                                                                  >  1:428899/1‑150 (MQ=255)                              tttCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTaa                                                                                                                 <  1:373456/150‑1 (MQ=255)                                 ccAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCaa                                                                                                             <  1:619311/151‑1 (MQ=255)                                   aGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAAc                                                                                                            >  2:318030/1‑150 (MQ=255)                                   aGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAAc                                                                                                            >  2:636201/1‑150 (MQ=255)                                   aGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACa                                                                                                           <  2:310032/151‑1 (MQ=255)                                         tCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttct                                                                                                     >  1:606775/1‑151 (MQ=255)                                           aCGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttctta                                                                                                   <  2:45398/151‑1 (MQ=255)                                            cGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttcttat                                                                                                  >  2:141219/1‑151 (MQ=255)                                                  ccGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTAc                                                                                            <  2:379475/151‑1 (MQ=255)                                                  ccGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTAc                                                                                            <  1:420944/151‑1 (MQ=255)                                                         tGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTa                                                                                      <  1:368175/150‑1 (MQ=255)                                                         tGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTa                                                                                      >  2:193161/1‑150 (MQ=255)                                                          ggAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTaa                                                                                     <  1:522720/150‑1 (MQ=255)                                                          ggAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAat                                                                                    <  2:136358/151‑1 (MQ=255)                                                            aaCCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAatat                                                                                  <  1:284668/151‑1 (MQ=255)                                                                     cGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCtt                                                                         <  2:346627/151‑1 (MQ=255)                                                                       acaacaATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCtttt                                                                       <  1:605071/151‑1 (MQ=255)                                                                               ccATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAAt                                                               <  2:648605/151‑1 (MQ=255)                                                                                cATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAAt                                                               >  2:32584/1‑150 (MQ=255)                                                                                    cGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACg                                                          >  1:293153/1‑151 (MQ=255)                                                                                     gAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACgg                                                         >  1:455572/1‑151 (MQ=255)                                                                                           gATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGAc                                                   >  1:143997/1‑151 (MQ=255)                                                                                                  tCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGCCAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTaa                                             >  2:343949/1‑150 (MQ=255)                                                                                                   ccACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTaaaa                                           <  1:360179/151‑1 (MQ=255)                                                                                                   ccACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTaaa                                            <  2:16158/150‑1 (MQ=255)                                                                                                    cACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTaaaaa                                          <  1:224147/151‑1 (MQ=255)                                                                                                         gcgcCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGc                                       <  1:424761/149‑1 (MQ=255)                                                                                                          cgcCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCata                                    <  1:658289/151‑1 (MQ=255)                                                                                                          cgcCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCat                                     >  2:583938/1‑150 (MQ=255)                                                                                                             cAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTc                                 <  1:161183/151‑1 (MQ=255)                                                                                                                           aGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCg                   >  2:98746/1‑151 (MQ=255)                                                                                                                            ggCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGa                  <  2:350512/151‑1 (MQ=255)                                                                                                                             gCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGaa                 <  1:509355/151‑1 (MQ=255)                                                                                                                                    ttttGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTa           <  1:162041/150‑1 (MQ=255)                                                                                                                                        gACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTaaaaaa      <  1:362186/151‑1 (MQ=255)                                                                                                                                            aTCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGc  <  2:312973/151‑1 (MQ=255)                                                                                                                                             tCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGc  <  2:585569/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               AAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGC  >  NC_000913/2129465‑2129754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑