Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,151,290	T→C	S36P (TCT→CCT)	yegJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,290	0	T	C	100.0%	99.4 / NA	28	S36P (TCT→CCT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/6);  total (22/6)

GTTTTTGCTAACTTTGACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGTCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCT  >  NC_000913/2151144‑2151436                                                                                                                                                   |                                                                                                                                                   gTTTTTGCTAACTTTGACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTg                                                                                                                                                 >  1:267237/1‑150 (MQ=255)   ttttGCTAACTTTGACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGAc                                                                                                                                               <  1:292401/150‑1 (MQ=255)                gACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATg                                                                                                                                  <  2:247366/150‑1 (MQ=255)                    aCAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCgg                                                                                                                             >  2:79440/1‑151 (MQ=255)                    aCAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCgg                                                                                                                             <  2:572832/151‑1 (MQ=255)                      aaaGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGga                                                                                                                            >  2:458496/1‑150 (MQ=255)                        aGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGgaa                                                                                                                           >  1:275269/1‑149 (MQ=255)                               cTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCg                                                                                                                   >  1:265179/1‑150 (MQ=255)                                tGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGAt                                                                                                                 >  2:467462/1‑151 (MQ=255)                                  gagaAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATa                                                                                                                >  2:132459/1‑150 (MQ=255)                                    gaAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAAt                                                                                                             >  2:222827/1‑151 (MQ=255)                                     aaaaaaTGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAAtt                                                                                                            >  2:210735/1‑151 (MQ=255)                                        aaaTGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCAt                                                                                                         >  1:319541/1‑151 (MQ=255)                                         aaTGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATc                                                                                                        >  2:630855/1‑151 (MQ=255)                                             aaaaaGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAgg                                                                                                    >  2:543865/1‑151 (MQ=255)                                                  gATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATg                                                                                               >  1:336598/1‑151 (MQ=255)                                                   atatTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATg                                                                                               >  1:532245/1‑150 (MQ=255)                                                      ttACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAAt                                                                                            >  2:241608/1‑150 (MQ=255)                                                       tACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATa                                                                                           >  2:602371/1‑150 (MQ=255)                                                       tACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATa                                                                                           >  1:594008/1‑150 (MQ=255)                                                            aTGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATaaaaaa                                                                                      <  1:176544/150‑1 (MQ=255)                                                                      tGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATAc                                                                           >  1:317790/1‑151 (MQ=255)                                                                                     ccGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGc                                                            <  1:15800/151‑1 (MQ=255)                                                                                          ggCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGa                                                       <  2:28948/151‑1 (MQ=255)                                                                                                             aTACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAAc                                    >  1:28689/1‑151 (MQ=255)                                                                                                                        aCGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCt                         >  1:259222/1‑151 (MQ=255)                                                                                                                                ttATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACg                 >  2:162279/1‑151 (MQ=255)                                                                                                                                                aTGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCt  >  2:650654/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GTTTTTGCTAACTTTGACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGTCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCT  >  NC_000913/2151144‑2151436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑