Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,168,009	T→C	T100A (ACA→GCA)	yegR ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,168,009	0	T	C	100.0%	125.3 / NA	33	T100A (ACA→GCA)	yegR	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/23);  total (10/23)

TGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAA  >  NC_000913/2167866‑2168151                                                                                                                                                |                                                                                                                                               tGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACg                                                                                                                                          >  1:151552/1‑150 (MQ=255)    ccACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCg                                                                                                                                      <  1:58692/151‑1 (MQ=255)                aaTCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAAt                                                                                                                          >  1:134041/1‑151 (MQ=255)                aaTCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAAt                                                                                                                          <  1:430981/151‑1 (MQ=255)                        ttCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACAcc                                                                                                                  >  1:647245/1‑151 (MQ=255)                          cATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACGcc                                                                                                                  <  2:15060/149‑1 (MQ=255)                             aGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCt                                                                                                              <  1:339224/150‑1 (MQ=255)                              gCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGt                                                                                                            <  1:51050/151‑1 (MQ=255)                                aCGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCt                                                                                                          >  1:77269/1‑151 (MQ=255)                                         ttCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATAGTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCa                                                                                                  <  2:558032/150‑1 (MQ=255)                                              ttATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTAtt                                                                                            >  2:525941/1‑151 (MQ=255)                                                   ggCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCAt                                                                                       <  1:26608/151‑1 (MQ=255)                                                     cATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCAttt                                                                                     <  2:571042/151‑1 (MQ=255)                                                       tCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCAttttt                                                                                   >  1:274520/1‑151 (MQ=255)                                                       tCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCAttttt                                                                                   >  2:105915/1‑151 (MQ=255)                                                              cccACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGccc                                                                             <  1:443957/150‑1 (MQ=255)                                                                  caGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGt                                                                        <  2:158123/151‑1 (MQ=255)                                                                            cTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGaa                                                               >  1:305080/1‑150 (MQ=255)                                                                              aCGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATg                                                            <  2:638816/151‑1 (MQ=255)                                                                                    aGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATACTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTg                                                       <  2:274449/150‑1 (MQ=255)                                                                                      ccAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGcat                                                    <  1:203576/151‑1 (MQ=255)                                                                                        aGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGcatca                                                  <  1:631483/151‑1 (MQ=255)                                                                                           aCGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCAttt                                               <  1:273853/151‑1 (MQ=255)                                                                                             gcgcATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCAttttt                                             <  1:397173/151‑1 (MQ=255)                                                                                               gcATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCAtttttt                                            >  1:559138/1‑150 (MQ=255)                                                                                                             tACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTg                               <  2:4824/149‑1 (MQ=255)                                                                                                              aCCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCa                             <  1:555029/150‑1 (MQ=255)                                                                                                                          tctcACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTa                 <  1:174580/150‑1 (MQ=255)                                                                                                                                   ccACATTTTAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCt        <  2:583082/150‑1 (MQ=255)                                                                                                                                   ccACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGc         <  1:522158/149‑1 (MQ=255)                                                                                                                                   ccACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCt        >  1:269871/1‑150 (MQ=255)                                                                                                                                   ccACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTa       <  1:354460/151‑1 (MQ=255)                                                                                                                                         ttGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAaagaa  <  2:630074/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               TGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAA  >  NC_000913/2167866‑2168151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑