Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,809,774	(T)7→6	coding (158/738 nt)	ygaZ →	putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,809,768	0	T	.	100.0%	122.6 / NA	26	coding (152/738 nt)	ygaZ	putative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/3);  total (23/3)

CCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACT  >  NC_000913/2809632‑2809906                                                                                                                                         |                                                                                                                                           ccACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGc                                                                                                                               <  1:600225/149‑1 (MQ=255)  cacaGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGcatc                                                                                                                            >  2:178810/1‑151 (MQ=255)   acaGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGcatc                                                                                                                            >  2:521523/1‑150 (MQ=255)      gCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGCTTACCGATTGTTATTAGTTATATTCAGGTGGCCTTTGCGTTAGGCCTGAATGCGACCCGTCTGGGATTCTCTCCTATCGAAAGCG‑TTTTTTCTCCTGCATCAttt                                                                                                                        >  2:428660/1‑151 (MQ=255)                      gCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                                                                          >  2:564184/1‑149 (MQ=255)                       cGACCCTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                                                                         >  2:111774/1‑149 (MQ=255)                           cTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGAATCATTTATGCAGGCGCGAGCCAGTTCg                                                                                                   >  2:537811/1‑151 (MQ=255)                              cATGGCAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                                                                 >  2:197727/1‑150 (MQ=255)                              cATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                                                                 >  2:28408/1‑150 (MQ=255)                                       aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgcg                                                                                        >  1:129506/1‑150 (MQ=255)                                       aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGa                                                                                       <  1:688014/151‑1 (MQ=255)                                           aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                                                                   >  2:287474/1‑151 (MQ=255)                                             aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                                                                 >  2:217682/1‑151 (MQ=255)                                                      aCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                                                                         >  2:148860/1‑150 (MQ=255)                                                             gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                                                                 >  1:32002/1‑151 (MQ=255)                                                                  taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                                                             >  1:620740/1‑150 (MQ=255)                                                                  taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                                                             >  2:11128/1‑150 (MQ=255)                                                                   aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt                                                           >  2:167189/1‑151 (MQ=255)                                                                      tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc                                                        >  2:213751/1‑151 (MQ=255)                                                                                          gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCa                                     >  1:673189/1‑150 (MQ=255)                                                                                          gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCa                                     >  2:371518/1‑150 (MQ=255)                                                                                                   gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTc                           >  1:348191/1‑151 (MQ=255)                                                                                                         gACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCAt                      >  2:290657/1‑150 (MQ=255)                                                                                                          aCCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCAtgt                    >  2:311342/1‑151 (MQ=255)                                                                                                             cGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGtgtt                 <  1:111776/151‑1 (MQ=255)                                                                                                                            cctctcGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACt  >  2:261517/1‑151 (MQ=255)                                                                                                                                         |                                                                                                                                           CCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACT  >  NC_000913/2809632‑2809906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑