| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,097,218 | (G)7→8 | intergenic (+389/+347) | ycdU → / ← serX | putative inner membrane protein/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,097,211 | 1 | . | G | 82.3% | 18.9 / 4.3 | 17 | intergenic (+382/+354) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (5/9); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
GCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑A‑GGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTC > NC_000913/1097067‑1097307 | gCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑A‑gggggg < 1:687047/151‑1 (MQ=33) tCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑A‑gggggggggtt > 1:659302/1‑146 (MQ=18) gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGACt < 1:1952/149‑1 (MQ=18) aCTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACCAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAAAATGGCGGTG‑AGGGGGGGGATTGACtc < 2:287531/132‑1 (MQ=255) ggCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGACTCGCTTCGCTCGTCCTG‑CGGGCAg < 1:606100/151‑1 (MQ=16) gTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTTCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCg < 1:280691/151‑1 (MQ=16) gTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGGCCCCCTTCCCCCCCCCCGCCGGGCAGcc > 2:711041/1‑150 (MQ=11) ccAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCa < 1:280122/151‑1 (MQ=18) gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTTTATTCGCTTCGCTCGCCCCG‑CGGGCAGCCCGCTCACTGCGTTTAc > 2:319778/1‑151 (MQ=11) gCTTCCCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACt < 1:735445/151‑1 (MQ=9) tcATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTTGAATCGCTTCGCTCGCCCCG‑CGGGCCGGCCCCCCCCTGCGGTCACGGGCTGTCCCACTg > 2:723859/1‑147 (MQ=11) aTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTc < 2:659263/151‑1 (MQ=14) ccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTTGAACCCCTTCGCTCGCCCCG‑CGGGCAGCCCCCCCACTGCGGTCCCGGTCTGTCCAACTGGCTGTCGcc > 1:621374/1‑148 (MQ=11) cGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGt < 1:344447/150‑1 (MQ=14) gtgtgtGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGG‑AGGGGGGGGATTGAACCCCTTTCTTCCCCCCC‑CGGGCGGCCCCCTCCCTGGGTTCCCGGTCTTTCCAACTGGCGGTCGccc > 2:552092/1‑145 (MQ=2) aaTACACGAAAAAAAGGCCCGTCCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑A‑GGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCg < 2:685411/151‑1 (MQ=14) aaTACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGTG‑GGGGGGGATTGACTCCCTTCGCTCTCCCTG‑CGGGCAGCCCGCCCACTGCGTTCACGGGCTGTCCAACCGGGTGTGGCCCGTTGTCGAAcccc > 1:723921/1‑151 (MQ=11) acacGAAAAAAAAGCCCGTGCTTTCGTACGAGCTCTTCTATAAATATGGCGGTG‑A‑GGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTc < 2:339546/151‑1 (MQ=14) acacGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGt < 1:418766/151‑1 (MQ=14) | GCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑A‑GGGGGGGATTGACTCGCTTCGCTCGCCCTG‑CGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTC > NC_000913/1097067‑1097307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |