Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913370,8760CG50.0% 45.4 / 29.0 43S200R (AGC→AGG) mhpB2,3‑dihydroxyphenylpropionate 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base C (18/3);  new base G (0/21);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 7.52e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGC  >  NC_000913/370728‑371023
                                                                                                                                                      |                                                                                                                                                   
cccGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATTCCCATATGCGCTACCGTCTGTTGGGGAGCg                                                                                                                                                    <  1:470246/150‑1 (MQ=255)
  cGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGa                                                                                                                                                 <  1:183657/151‑1 (MQ=255)
   gCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGa                                                                                                                                                 >  1:399639/1‑150 (MQ=255)
   gCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGAGGGa                                                                                                                                                 >  1:490524/1‑150 (MQ=255)
                   cATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTCT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAg                                                                                                                                 >  2:398073/1‑150 (MQ=255)
                    aTTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTg                                                                                                                               >  2:41503/1‑151 (MQ=255)
                           gTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGcgcg                                                                                                                         >  1:158482/1‑150 (MQ=255)
                            tttCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGa                                                                                                                        >  1:656435/1‑150 (MQ=255)
                                aCCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGc                                                                                                                   >  1:9588/1‑151 (MQ=255)
                                aCCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGc                                                                                                                   >  1:638920/1‑151 (MQ=255)
                                         caaaaaaaccgcgTGTTGTT‑CTGGGTTCCGGGGGGGCTTC‑CCCATCGGCCGCGGGTGCGAAACGGGGCAAAGGCCGCTCCCCATATGCGGGACCGTTGGTGGGGGGGGGGAAAAGTTTCCCCCGCCAGTGAGCGCGAATTGCGTCAGCa                                                                                                            <  1:573796/140‑1 (MQ=255)
                                            aaaaaccgggtgtTGTTCC‑GGGTTTC‑GGGGGGTTTTCCCCACCGCCCGCGGGTGCCCGAAGGGGCGAAGGCCGAGCCCCATAAGCGCGCCCTTCTGGGGGGGGGGGGGAAAAATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACgg                                                                                                        <  1:591709/141‑1 (MQ=255)
                                            aaaaaccgcgTGTTGTTCGGGGGTTCG‑GGTGGGCTTC‑CCCACCGGCCGCGGGTGCCCGAACGGGCGAAAGCCGATCCCCATATGCGCGCCCGTTTGTGGGGGAGCGGAAAAGTTTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAAc                                                                                                          <  1:542860/142‑1 (MQ=255)
                                            aaTAACCGCGGGTTTGTCGTGGTTTCG‑GGTGGCTTTC‑CCCACCGGCCGCGGGTGCGGGAGCTGGCAAAAGCCGAGCCCCATGGGCGCGCCTGTCTGGGGGGGGGGGGGAAAAATTTCCCCGCCAGTGAGCGCGAATTGCg                                                                                                                  <  2:35767/140‑1 (MQ=255)
                                                   gcgggttGTTCGTGGTTTCG‑GTTGGTCTTC‑CCCCCCCGCCGCGGGGGCCAGATCGGGGAAAGGCCGATGCCCATATGCGCGGCGTTGTGTGGGGGGGGGGGAAATTTTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGAtt                                                                                                  <  2:712942/143‑1 (MQ=255)
                                                      tgcttgtcgtGGGTTCG‑GGTGGCTTTT‑CCCAGCCCCCGCGGGGGCCCGAGGGGGAGAAGGCCGAGCCCCATTTGCGCGCTGATGTGGGGGGGGGGGGGAAAGTTTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAgcg                                                                                              <  2:443979/141‑1 (MQ=255)
                                                             ccTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTg                                                                                        >  2:301884/1‑149 (MQ=255)
                                                                gggTCCG‑GGTGGCTTTC‑CCCACCGGCCCCGGGTGCCGGAACGGGCAAAGGCCGATCCCCATAGCCGCGCCCGTGTGTTGGGGGGGGGAAAAGTTTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAg                                                                                   <  1:238403/151‑1 (MQ=255)
                                                                   ttCC‑GGTGGGTTTT‑CCCATCCGCCGCGGGTGCCGGAGCTGGCAAACGCCGGTGCCTATTGGCGCGCCCGTCTTTTGGGGAGGGGAAAAGATTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGt                                                                                  <  1:38161/149‑1 (MQ=255)
                                                                        ggggggCTTC‑CCCACCGGCCGCGGGGGCCCGAGCTGGAAAAGGCCGATCCCCATAGGCGCGCCCGTCTGTGGGGGGGGGGAAAATATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTttg                                                                               <  1:754836/145‑1 (MQ=255)
                                                                        ggTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTttgttg                                                                            >  1:478072/1‑151 (MQ=255)
                                                                        ggTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTttgttg                                                                            >  2:501612/1‑151 (MQ=255)
                                                                         gTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGGGATTAGCGCCGCTGAGAAGTttgttg                                                                            >  2:704885/1‑150 (MQ=255)
                                                                                ttccccACCGGCCGCGGGGGCCCGAACGGGGAAAAGCAGATCCCCATAGGCGCGCGCGTCTGGGGGGGGGGGGGAAAATTTTGCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGa                                                                        <  2:532719/145‑1 (MQ=255)
                                                                                             gccgGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCAt                                                        >  2:529889/1‑151 (MQ=255)
                                                                                                ggTGCCCGAAGGGGAGAAGGCCGATCCCAATAGGCGCGCCCGTTTGTGGGGGGGGGGAAAAGTTTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCt                                                            <  1:6120/144‑1 (MQ=255)
                                                                                                        aatgggaaaaaGCCGTTGCCAATAGGCCCGCCGGTTGGTGGGGGGGGGGAAAAGTTTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCa                                                 <  1:221421/139‑1 (MQ=255)
                                                                                                           gggaaaacgCCGATGCCCATAGGCGCGCCGGTGTGTGGGGGGGGGGGAAAGTTTCCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCg                                            <  2:691628/141‑1 (MQ=255)
                                                                                                                agtcccGATCCCTATGGGCGCGCCGGTCGGGGGGGGGGGGGGAAAGTTTCCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTgg                                      <  2:505100/146‑1 (MQ=255)
                                                                                                                aaaGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTgg                                      >  1:596551/1‑150 (MQ=255)
                                                                                                                    ccGATCCCCATAGGCGCGACCTTCTGTTGGGGAGGGGAAAAGTTTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATa                                  <  1:106414/150‑1 (MQ=255)
                                                                                                                        tGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAAcc                               >  2:345230/1‑149 (MQ=255)
                                                                                                                             atagGCGCGCCCGTGTGGTGGGGGGGGGAAAATATTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTc                          <  1:101289/149‑1 (MQ=255)
                                                                                                                              tatGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATg                       >  1:336386/1‑151 (MQ=255)
                                                                                                                                tGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGAc                     >  1:31642/1‑151 (MQ=255)
                                                                                                                                  cgcgCCCTTTTGTGGGGGGGGGGAAAAGTTTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACtt                   <  1:191407/151‑1 (MQ=255)
                                                                                                                                   gtgacgggttggtGGGGAGGGGAAAAGATTGACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGGTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACtt                   <  1:73065/138‑1 (MQ=255)
                                                                                                                                    cgcccGTTTGTGGGGGGGGGGAAAATTTTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGAc                     <  1:32855/144‑1 (MQ=255)
                                                                                                                                    cgACCGTTGGTGGGGGGGGGGAAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTc                          <  1:506143/142‑1 (MQ=255)
                                                                                                                                        cGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGGATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTgg             >  2:432305/1‑151 (MQ=255)
                                                                                                                                          tgtgtgGGGGAGGGGGAAAGATTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGa            <  1:192751/148‑1 (MQ=255)
                                                                                                                                               gggggggggGAAAAGATTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAgg       <  1:685829/149‑1 (MQ=255)
                                                                                                                                                   gAGGGGGAAAGATTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGc  <  1:473885/151‑1 (MQ=255)
                                                                                                                                                   gAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGc  >  1:249677/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
CCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCC‑GGTGGGCTTT‑CCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGC  >  NC_000913/370728‑371023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑