Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913646,7600TC50.0% 28.3 / 10.4 35K250K (AAA→AAG) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base T (15/2);  new base C (17/0);  total (33/2)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGT‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGC  >  NC_000913/646612‑646907
                                                                                                                                                      |                                                                                                                                                   
ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGT‑GCGAAGCCCCCCtt                                                                                                                                                    >  2:536971/1‑151 (MQ=255)
ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAATGCCCCCctt                                                                                                                                                   >  2:139117/1‑151 (MQ=255)
ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAATGCCCCCctt                                                                                                                                                   >  1:530721/1‑151 (MQ=255)
    cAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAATACCCCCTTTTTgg                                                                                                                                               >  1:513351/1‑150 (MQ=255)
                  atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TTGAATGCCCCCCTTTTTCaataaaattt                                                                                                                                     >  2:408494/1‑142 (MQ=255)
                    tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTGTGGATAGCCCCCATTTTTCAA‑AATGtttt                                                                                                                                    >  2:42519/1‑145 (MQ=255)
                    tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGT‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGcc                                                                                                                                <  2:530695/150‑1 (MQ=255)
                    tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGT‑TCGAAAGCCCCCCTTTTTCAATAATGtttttgcc                                                                                                                                >  1:404561/1‑145 (MQ=255)
                      aaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCCAATGACCCCCTTTTTCAATAATTTTTTCGcccc                                                                                                                              >  2:352381/1‑148 (MQ=255)
                         cTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTAGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCTCAATCCCCCTTTTTGTACAAATATTTGCGcccc                                                                                                                              >  2:491639/1‑145 (MQ=255)
                                    aCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAATGCCCCCCTTTTTCAAAAATTTTTTCGCCTCGCGCCGTAGcc                                                                                                                   >  2:450800/1‑147 (MQ=255)
                                      cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGT‑TTTAAT‑CGCCCTCTTTTCAAAAATATTTTGGCCACGCGCCGtttccca                                                                                                                 >  2:311794/1‑141 (MQ=255)
                                      cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TTGAATGCCCCCCTTTTTCCATAATATTTTTGCCTCGCGCTGTAGCCAgcgc                                                                                                              >  1:312472/1‑150 (MQ=255)
                                      cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCCGTAGcc                                                                                                                   >  1:235049/1‑145 (MQ=255)
                                            aGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAATGCCCCCCTTTTTCAAAAATGGTTGTGGGTCGGGCTTGAGGCAGCGGAggggcc                                                                                                       >  2:66011/1‑147 (MQ=255)
                                                 cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAATTCCCCCCTTTTTTAATAATGTTTGTGCCCCCCCCCGTAGCCCACGCAGGcccccccc                                                                                                   >  1:313195/1‑148 (MQ=255)
                                                          aGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAATGCCCCCCTTTTTTAATAAATTTTTCGCCCCCCGCTGTAGCCAGCGCCGGcccccccccccccccccc                                                                                         >  1:630300/1‑139 (MQ=255)
                                                                   gccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGT‑TCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCcccgc                                                                                         >  2:161016/1‑142 (MQ=255)
                                                                             agaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGAAAGCCCCCCTTTTTCAATAAAGTTTTCGCCCCGCGCTGTCGGCCGCGCCGGCCCCCCCCCCCCCCCCGCGATGCAACGtttggat                                                                         >  1:390402/1‑143 (MQ=255)
                                                                                  tcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGT‑TTGAAAGCCCCCCTTTTTGAATAATTTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCCCCACCCCCCGCTGCAACGTTGGGATCGc                                                                      >  1:54461/1‑146 (MQ=255)
                                                                                      tcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TTGAATGCCCCCCTTTTTTAATAAATTTTTCGCCCCGCGGCGTTGCCAGCGGAGGGCCCCCCCCCCCCCCCGCGATGCCACGCTGGTATCGCCGGTGAttg                                                             >  1:651109/1‑149 (MQ=255)
                                                                                      tcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TTGAAAGCCCCCCCTTTTTCATAAATTTTTTGCCCCGCCCTGGTGGCCGCGCAGGCCCCCCCCGCCAACCCCCGATGCAACCTTGGGTTCGCCGGTGAt                                                               >  1:108238/1‑149 (MQ=255)
                                                                                           acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGG‑TCGCATGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCGCGATGCAACGTTGGGATCGCCGCTGATCGcc                                                           >  1:355945/1‑148 (MQ=255)
                                                                                                       gAACTGCCGGAGATAATCGAGATCGGC‑GGGGGT‑TCCAATTCCCCCCTTTTTCAATAAAATTTTCGCCCCCCGCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGCTGCAACGCTTGTATTGCCCGTTATTGCCCTCCGTAgg                                                  >  1:710379/1‑145 (MQ=255)
                                                                                                        aaCTGCCGGAGATAATCGAGATCGGC‑GGGGGT‑TTGAATGGCCCCCTTTTTCAATAATGTTTTCGCCCCCCCCCGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCCCGCTGCCACGGTGGTATCGCCCGTGATCGCCCTCCGTAGGGGCAAgg                                           >  1:30760/1‑151 (MQ=255)
                                                                                                                          agaTCGGC‑GGGGGT‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCa                            <  1:754018/148‑1 (MQ=255)
                                                                                                                             tCGGC‑GGGGGT‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTCGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGGTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTc                        >  2:59864/1‑149 (MQ=255)
                                                                                                                             tCGGC‑GGGGGG‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTc                        >  2:110850/1‑149 (MQ=255)
                                                                                                                                     gggT‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTa                >  2:637006/1‑150 (MQ=255)
                                                                                                                                     gggT‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTa                >  2:538476/1‑150 (MQ=255)
                                                                                                                                       gT‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAcc             >  1:591721/1‑151 (MQ=255)
                                                                                                                                            gAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTg         >  2:175719/1‑151 (MQ=255)
                                                                                                                                              aTGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGAt       >  2:447334/1‑151 (MQ=255)
                                                                                                                                                gCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATcc     >  1:350030/1‑151 (MQ=255)
                                                                                                                                                  ccccTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATcc     >  1:194124/1‑149 (MQ=255)
                                                                                                                                                   cccTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagc  >  2:10963/1‑151 (MQ=255)
                                                                                                                                                     cTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagc  >  2:336429/1‑149 (MQ=255)
                                                                                                                                                     cTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagc  >  2:149210/1‑149 (MQ=255)
                                                                                                                                                      tttttGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagc  >  1:609148/1‑148 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGT‑TCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGC  >  NC_000913/646612‑646907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑