Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,581	0	G	T	52.6%	6.5 / 27.6	19	D133Y (GAT→TAT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base T (10/0);  total (14/5)
Fisher's exact test for biased strand distribution p-value = 1.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-02

ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCC  >  NC_000913/2151443‑2151729                                                                                                                                           |                                                                                                                                                       aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGA‑TCCTTTATTgg                                                                                                                                           <  1:479165/151‑1 (MQ=255)        ttGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACATTTATTCCTTTTTTGTTCAc                                                                                                                                       >  2:573174/1‑149 (MQ=255)                         gACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATTTCATTGT‑TTTTTTTTTTGTTGACTATA‑CAAaaaaaga                                                                                                                       >  2:649154/1‑146 (MQ=255)                         gACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCcg                                                                                                                  <  1:25567/151‑1 (MQ=255)                            ccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACATTTA‑TTCTTTTTTTGTTACCTCTA‑CAAaaaaagaa                                                                                                                      >  2:174271/1‑144 (MQ=255)                                         cAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCCGACGAGCTAAGAGCaaa                                                                                                  <  1:402971/151‑1 (MQ=255)                                            aCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAtt                                                                                               <  2:203077/151‑1 (MQ=255)                                                aGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCCTTTA‑TTCTTTTTTTGTCACCCCTC‑CCAAAAAAAAAACCGACGAGCTAAAAGGAAAATTAGt                                                                                            >  1:1826/1‑150 (MQ=255)                                                  aaTATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACATTTA‑ACCTTTTTTGGGCACCCTTT‑CTAAAAAAAAAGACCACGAGCTAAGAGCaaaa                                                                                                 >  1:65024/1‑143 (MQ=255)                                                               gTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACATTTT‑TCCTTTTTTTGTTACCTATCTCAAAAAAAAAAGCAG‑CGAGGTAAGAGGAAAAATATTTTTCTTATCaagc                                                                               >  2:120044/1‑145 (MQ=255)                                                               gTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTA‑TCCTTTATTTGTCACCTATA‑CAAAAAAAAAAACCGACGAGCTAAGAGAAAAAATAATTTGCTAATCAgac                                                                               >  1:15042/1‑146 (MQ=255)                                                                             aagaagATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACATTTA‑TCCTTTTTTTGGTTCCCATC‑CCAAAAAAAAAAGCGACGAGGTAAAAGGAAAAATAGTTTGCTAATTAACCGGTATAttttttttttt                                                              >  1:6788/1‑150 (MQ=255)                                                                               gaagaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACCTTTA‑TCCTTTTTTTGTTCCCTAAC‑CAAAAAAAAAAAACGCCAAGCTAAAAGGAAAAATAATTCGCTTAACAACCGTTAtttttttttttt                                                               >  2:254754/1‑148 (MQ=255)                                                                                     atTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGc                                                       <  1:56382/150‑1 (MQ=255)                                                                                      tttCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTACCATTTT‑TCCTTTTTTTGTCACCTATC‑CAAAAAAAAAAAACCACGAGCTAAGAGCAAAAATAATTTGCTAATTAGCCGTTATATTTATTTTTTTAAATTGCgg                                                     >  1:116974/1‑151 (MQ=255)                                                                                                 ctGTATACGCGCGGGGATAAAATTTACGGCGGTTATTCCATTTA‑TTCTTTTTTTGTCACCTATC‑CAAAAAAAAAAAACGACCAGCCAAGAGAAAAAATAATTTGCTAATAAGCCGTTATATTTATTTTTTTAAATAGCgg                                                     >  2:684878/2‑140 (MQ=255)                                                                                                                                  tataCCATTGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAAACGCCATGAGAAAATCACTCTTccc          >  1:85841/1‑150 (MQ=255)                                                                                                                                     aCCATTGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACCGGCGGTTGGATTGTTTTCTTCAACCGCCCTGAGAAAATCACTCTGccc          >  1:142770/1‑147 (MQ=255)                                                                                                                                         ttGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGcc  >  1:92791/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                       ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGA‑TCCTTTATTGGTCACCTATC‑CAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCC  >  NC_000913/2151443‑2151729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑