| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,628,625 | 0 | A | C | 53.8% | 5.5 / 14.3 | 14 | V105G (GTC→GGC) | yfgF | cyclic‑di‑GMP phosphodiesterase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/7); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
GCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAT > NC_000913/2628486‑2628747 | gCTCGCCAGTAATCCTACAAATGCAGAAAACTGGAAAAGTATCAAAAACAGCGTTGCAGGAAACGCAATCTGCCAGAATATGCGCTAGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTGCGCCGGGGAATTAAGGCCCTGTa < 2:383409/147‑1 (MQ=255) ccAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGcc > 1:183364/1‑148 (MQ=255) ccAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGcc < 2:183357/148‑1 (MQ=255) aaTCCTACAAATGCAGCAAACGGGAAAAGTATCAGAAACAGCGTGGCAGGAAACACAATCTGCCAGAATATACGCGGGGAAATAAACCGGGTACCCCCATGTAAACCGTTGTTACGCCGGGGAGTAAAGCCCCTGTAACCGCCCCAACag < 2:199934/150‑1 (MQ=255) cTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaat > 1:160704/1‑151 (MQ=255) gcaAACGGGAAAAATATCAGAAACAGTGTTGCAGGAAACACAATCGCCCAAAATATCCGCTGGGAAATTAAACGGTTATCCCCATGTGAAACGTTATTACGCCGGGAAAAAAAGCCCCAGTAGCCGCCCCAACAGAGAAtgataatgataa < 1:132160/151‑1 (MQ=255) caAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATgataatgataat > 1:740199/1‑151 (MQ=255) aaaCGGAAAAAGTATCAGAAACAGCGTGGCAGGAAACACAATCTGCCAGAATATCCGCTGGAAAATAAACCGGGTACCCCCAGGTGAAACGTTTTTACGCGGGGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGaatgataatg < 2:621714/145‑1 (MQ=255) cTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATaaa > 1:689076/1‑150 (MQ=255) aGAAACAGCGTTGAAGAAAACACAATCTGCCAAAATATACGCGGGGAAATTAAACGGGTATCACCATGTAAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTa < 1:242477/148‑1 (MQ=255) gctttGCAGGAAACACAATCTGCAAAAATATCCGCTGGAAAATTAAACGGTTATCCCCTTGTAAAACTTTTTTCCGCCGGGGATTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGc < 1:2899/146‑1 (MQ=255) aaTATACGCTGGGAAATTAAACGGGTATCACCATGTAAAACTTTGTTCCGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCat < 1:518305/148‑1 (MQ=255) ataaCCATGTGAAACTTTGTTCCGCCGGGAAGAAAAGCCCCGGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGAt < 2:427946/147‑1 (MQ=255) aaaCGTTGTCACGCCGGGGATTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAt < 2:701635/149‑1 (MQ=255) | GCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAT > NC_000913/2628486‑2628747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |