Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,7480CA56.1% 18.7 / 59.2 41E55* (GAG→TAG) nudFADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (13/5);  new base A (0/23);  total (13/28)
Fisher's exact test for biased strand distribution p-value = 4.86e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-07

CTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGC  >  NC_000913/3177600‑3177890
                                                                                                                                                    |                                                                                                                                              
cTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTC‑AAGGGGAGCAAGACTGCGGCGTGACCGCGCTCaa                                                                                                                                              >  2:188168/1‑150 (MQ=255)
    cACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAAt                                                                                                                                          >  1:117184/1‑151 (MQ=255)
      cccTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAAttt                                                                                                                                        >  2:696967/1‑151 (MQ=255)
         tctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTccc                                                                                                                                     >  2:690321/1‑151 (MQ=255)
                 catcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCAc                                                                                                                              <  1:638499/150‑1 (MQ=255)
                                         ccAAGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTAAAAGGGTAGCAAGACGGCGGCGTGACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGa                                                                                                       <  2:757705/149‑1 (MQ=255)
                                           aggggtTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACACCTCCACCACGCACGGGGTAAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                                                                                                    <  2:344759/148‑1 (MQ=255)
                                                 tttCGCTGGTGTCGTCCGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                                                                                              <  1:365946/150‑1 (MQ=255)
                                                    cGCTGGTGTCGTACGCGGCAACCCAAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGCCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                                                                                           <  1:312152/150‑1 (MQ=255)
                                                    cGCTGGTGCCGTCCGGGGCAACCCGAATCTCCCAAACCACCACAATTTCACCCCGCACGGGGTAAAGGGGTAACAAGACTGCGGCGTGACCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACg                                                                                          <  2:360388/151‑1 (MQ=255)
                                                            tCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTc                                                                                                                                       <  1:138638/98‑1 (MQ=255)
                                                            tCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTc                                                                                                                                       >  2:138635/1‑98 (MQ=255)
                                                             cGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                 <  1:20796/151‑1 (MQ=255)
                                                              gTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAGGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt                                                                                    <  1:222987/147‑1 (MQ=255)
                                                              gTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                <  2:756084/151‑1 (MQ=255)
                                                                  gcgGCAACCCAAATCTGCACAACCAGCACAACTCCATCACGCACTGGGTCAAGGGGTAGCAAGACTGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGa                                                                             <  2:244470/150‑1 (MQ=255)
                                                                       aaTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAt                                                                       >  2:606307/1‑151 (MQ=255)
                                                                        aTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg                                                                      >  1:160613/1‑151 (MQ=255)
                                                                          ccGAATCTGCTCAATCAGCACAACTTCATCACGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                    <  1:684882/151‑1 (MQ=255)
                                                                          ccGAATCTGCTCAATCAGCACAACTCCATCACGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg                                                                      <  1:315796/149‑1 (MQ=255)
                                                                             aaTCTGCTCAATCAGCACAACTACATCACCCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCCCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaa                                                                  <  1:322673/150‑1 (MQ=255)
                                                                                 tGCTCAACCACCACAACTCCACCCCGCACTGGTTCAAGGGGTAGCAAGCCGGCGGCGTGCCCGCCCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg                                                                      <  2:668353/142‑1 (MQ=255)
                                                                                    tCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGcc                                                            >  2:428166/1‑149 (MQ=255)
                                                                                      aaTCAGCACAACTACATCACGCATTGGTTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgcg                                                         <  2:133821/150‑1 (MQ=255)
                                                                                       aacAGCCCACCTCCATCCCCCACTGGGTCAAAGGGTAGAAAGACTGGGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt                                                       <  2:258814/149‑1 (MQ=255)
                                                                                          aGCACACCTTCAACACGCACTGGGTAAAGGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATaa                                                     <  1:172548/150‑1 (MQ=255)
                                                                                                 cTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtc                                             >  2:31783/1‑151 (MQ=255)
                                                                                                       cacgcacgGGGTCAAAGGGTAGCAAGACGGCGGCGTGACCGCGCAAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTg                                        <  2:600571/150‑1 (MQ=255)
                                                                                                        aCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGc                                       >  1:275674/1‑150 (MQ=255)
                                                                                                         cGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCa                                      <  1:764618/150‑1 (MQ=255)
                                                                                                          gCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                    >  2:700996/1‑151 (MQ=255)
                                                                                                           cACGGGGTAAAAGGGTAGAAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaata                                   <  1:697009/151‑1 (MQ=255)
                                                                                                            aCTGGGTAAAAGGGTAGAAAGCCGGCGGCGTGCCCGCACCAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaata                                   <  2:292186/150‑1 (MQ=255)
                                                                                                              tGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataa                                  <  1:245979/149‑1 (MQ=255)
                                                                                                               gggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAAttt                               <  1:303463/151‑1 (MQ=255)
                                                                                                                ggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTc                              <  1:297519/151‑1 (MQ=255)
                                                                                                                              acgACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt                  <  2:275663/147‑1 (MQ=255)
                                                                                                                              aaGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt                  <  1:690363/149‑1 (MQ=255)
                                                                                                                                    gcggcgTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCaaa            <  1:441259/149‑1 (MQ=255)
                                                                                                                                           gACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACggg   >  2:16477/1‑151 (MQ=255)
                                                                                                                                              cgcgCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc  >  2:4504/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGC  >  NC_000913/3177600‑3177890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑