Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,573,496 | 0 | A | G | 51.0% | 32.0 / 75.8 | 50 | pseudogene (1083/1503 nt) | yjiT | pseudogene |
Reads supporting (aligned to +/- strand): ref base A (4/20); new base G (25/0); total (30/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.76e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.16e-09 |
CATTTGTGAAGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATTGTGCTCC > NC_000913/4573346‑4573644 | cATTTGTGAAGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGa < 1:281450/151‑1 (MQ=255) aGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GGTGGGGGGGGGA‑tt > 1:364744/1‑151 (MQ=255) gATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑ttg < 2:356643/151‑1 (MQ=255) aaGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GGTGGGGGGGTGG‑TTT‑TT‑tgggggat > 1:151110/1‑146 (MQ=255) ctctGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GCTGGGGGGGTGA‑TTG‑TT‑GGGGGCA‑tt > 1:175725/1‑148 (MQ=255) gCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGGGGGGTGG‑TTG‑TT‑GGGGGGA‑TTAAAAtt > 1:165068/1‑149 (MQ=255) gggCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACa < 2:493252/151‑1 (MQ=255) aGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GGTGGGGGGGTGA‑TTG‑TT‑GGGGGGA‑TTAAACTTGATGAAA‑ggg > 1:691050/1‑147 (MQ=255) aGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GGTGGGGGGGTGA‑TTT‑TT‑GGGGGCA‑TTAAAAtt > 1:239546/1‑139 (MQ=255) gCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCAT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑cc < 2:599754/150‑1 (MQ=255) ctGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GGTGGGGGGGGGG‑TTG‑TT‑GGGGGGA‑TTAAAATTGATGACA‑GT‑G‑AAA‑TTG‑GCATTGGT‑g > 1:145273/1‑151 (MQ=255) ggAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGt < 2:343677/148‑1 (MQ=255) aaaaCAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GGTGGGGGGGAGA‑TTT‑TT‑GGGGGGATTTAAATTTGTTG‑CA‑GTGG‑AAA‑TTG‑CATTTggggggg > 2:74719/1‑146 (MQ=255) aaaCAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGACTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAgg < 2:37042/149‑1 (MQ=255) agacagGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCCG‑GGTGGGGGGGGGG‑TTT‑TT‑GGGGGGA‑TTAAAATTGTTGGCAGGG‑G‑AAA‑TGG‑CCTTTgggggggg > 2:6226/1‑141 (MQ=255) acagGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGGGGGGGGA‑TTG‑TT‑GGGGGGA‑TTTAAATTTATGACA‑GTGG‑AAA‑TTT‑CCTTTGG‑‑GAGGGGCCGtt > 1:553682/1‑150 (MQ=255) acagGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCCG‑GGTGGGGGGGGGA‑TTT‑TT‑GGGGGGA‑TTAAAATTTTTGACA‑AG‑G‑AAA‑ATG‑GCATTTGG‑GAgggggcggt > 2:691385/1‑143 (MQ=255) agGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GGTGGAGGGGTGA‑TTG‑TT‑GGGGGGA‑TTAAAATTGATGACA‑GG‑G‑AAA‑ATG‑CCATTGGT‑gggggggcggt > 1:247499/1‑141 (MQ=255) agGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GGTGGGGGGGAGATTTT‑TT‑GGGGGAT‑TTAAACTTGTTGA‑A‑GG‑G‑AAATTTG‑CCTTTgggggggggcggt > 2:743209/1‑137 (MQ=255) aaCAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GCTGGGGGGGTGG‑TTT‑TT‑TGGGGCA‑TTAAAATTTTTGACA‑GT‑G‑AAA‑TTT‑CCCTTGGG‑GGGGGGCCGTTTACCtt > 1:418995/1‑150 (MQ=255) cGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GGCGGGGGGGTGG‑TTG‑TT‑TGGGGCA‑TTAAACTTTCTGGCA‑GT‑GAAAA‑TGG‑CCTTTGGG‑GGGGGGCGTTTAACTTTctcctt > 1:392994/1‑145 (MQ=255) cGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTg < 1:134986/151‑1 (MQ=255) gATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GGTTGGGGGGAGA‑TTG‑TT‑GGGGGGA‑TTATAATTGTTGGAA‑CT‑G‑AAA‑ATG‑TCCTTGGG‑GGGGGGGCGTTAAACTTccttctt > 2:552689/1‑143 (MQ=255) gATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GCTGGGGGGGTGA‑TTG‑TT‑GGGGGGA‑TTAAACTTGATGACA‑GG‑G‑AAA‑TTG‑CCCTTGGG‑GAGGGGGCGTTTAACTTCAttgttg > 1:283532/1‑150 (MQ=255) tACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATg < 1:113736/150‑1 (MQ=255) aCGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCa < 1:229372/151‑1 (MQ=255) aCGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GGTGGGGGGGTGA‑TTG‑TT‑GGGGGGA‑TTAAAATTTGTTACA‑GT‑G‑AAA‑ATG‑TCCTTTGG‑GGGGGGCCGTTTACCCTCCTCGtttgt > 1:239988/1‑145 (MQ=255) cGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GGTGGGGGGAGAA‑ATA‑TT‑GGGGGGA‑TTAAAAATTATGAAC‑AT‑G‑AAA‑TTT‑GCATTGGG‑GGGGGGGCGGTAACCTTTATAGTTGAt > 2:200560/1‑147 (MQ=255) aaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGGCTGG‑GGGGGGA‑TTT‑TTGGGGGGCT‑TAAAACTTTTGGAAG‑GG‑G‑AAA‑TTG‑‑CATTGGT‑GGGGTGGCGTTTAACTTCCTCGTTTATGCAggt > 2:415518/1‑146 (MQ=255) aaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GCTGGGGGGGTGG‑TTG‑TT‑TGGGGGA‑TTTAAATTTGTGACA‑GT‑G‑AAA‑ATT‑CCCTTGGTGGGGGGGCCGTT‑ACCTTCATCGTTTATGCa > 1:253438/1‑145 (MQ=255) gCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGGGGGGTGG‑TTG‑TT‑TGGAGCA‑TTAAAACTTATGACA‑GT‑G‑GAA‑ATG‑CCCTTGGG‑GGGGGGCCGTTAACCCTTATCGTTGGTGGAGAACaa > 1:583535/1‑149 (MQ=255) gCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg < 2:24388/151‑1 (MQ=255) gAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGGGGGGTGG‑TTG‑TT‑TGGAGCA‑TTAAAATTTATGACA‑GT‑G‑GAA‑TTT‑CCATTTGT‑GGGGGGCCGTTTAACTTCATCGTTGATGCAGATCAATgg > 1:292303/1‑150 (MQ=255) gagaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGtt < 2:648181/150‑1 (MQ=255) ttGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GGTGGGGGGGTGA‑TTGTTT‑GGGGGCT‑TTAAA‑TTTATGAAAGGG‑G‑AAA‑TTG‑CCTTTGGG‑GGGGGGCGGTTAA‑CTTTATCTTTTATGGAGATCAATTGCTGTTTCAggg > 1:608648/1‑151 (MQ=255) ggcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGAc < 1:66107/151‑1 (MQ=255) aggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GGTGGGGGGGTGA‑TTT‑TT‑GGGGGGA‑TTTAAATTTATGAAA‑GG‑G‑GAA‑ATG‑CCCTTGGG‑GAGGGGCCGTTAAACTTCATCGTTGATGGAGATCAATGGGTGTTTCAGGGGCAgg > 1:651173/1‑151 (MQ=255) caAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGctt < 1:6226/151‑1 (MQ=255) caAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGct < 1:46211/150‑1 (MQ=255) aaaaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCGGGGGGGGGGG‑TTT‑TT‑GGGGGCA‑TTTAAATTTTTGACA‑GT‑G‑AAA‑ATGTCCTTTGTG‑GGGGGGCCGTT‑ACCTTTCTTGTTTATGCCGATAAAAAGCTGTTTTAGGGGCAGGctt > 2:466306/1‑150 (MQ=255) aaaaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGG‑GCTGGGGGGGTGA‑TTG‑TT‑GGGGGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑ATT‑CCATTGGG‑GAGGGGCCGGTAAACTTCATCGTTGTTGCAGAGAAAAGGCTGTTTAAGGGACAGGctt > 1:228554/1‑150 (MQ=255) aGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGa < 1:440609/151‑1 (MQ=255) tGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagca < 1:693033/150‑1 (MQ=255) gTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagcagc < 1:291005/151‑1 (MQ=255) gTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagcagc < 1:346905/151‑1 (MQ=255) gTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagcag < 2:591745/150‑1 (MQ=255) gTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGGGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGGGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGtt > 2:730496/1‑150 (MQ=255) cTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGAt > 1:264030/1‑151 (MQ=255) cGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATtg < 2:36630/151‑1 (MQ=255) gT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATtgt > 1:498111/1‑151 (MQ=255) ggAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATTGTGCTcc < 1:658277/151‑1 (MQ=255) | CATTTGTGAAGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGT‑GCTGGAGGGATGA‑TTG‑TT‑GGGAGCA‑TTAAACTTGATGACA‑GT‑G‑AAA‑TTG‑CCATTGGT‑GAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATTGTGCTCC > NC_000913/4573346‑4573644 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |