Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	216,672	A→G	V111A (GTC→GCC)	yaeF ←	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,672	0	A	G	100.0%	156.4 / NA	42	V111A (GTC→GCC)	yaeF	putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

GCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGACGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGT  >  NC_000913/216533‑216818                                                                                                                                            |                                                                                                                                                   gcgATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCt                                                                                                                                         >  1:727978/1‑151 (MQ=255) gcgATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCt                                                                                                                                         <  1:123210/151‑1 (MQ=255) gcgATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCt                                                                                                                                         <  1:611433/151‑1 (MQ=255)  cgATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTg                                                                                                                                        <  1:668220/151‑1 (MQ=255)    aTAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTc                                                                                                                                      <  2:203001/151‑1 (MQ=255)    aTAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTc                                                                                                                                      >  2:477075/1‑151 (MQ=255)    aTAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTc                                                                                                                                      >  1:638360/1‑151 (MQ=255)            aaCCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGc                                                                                                                              <  1:570432/151‑1 (MQ=255)                  tATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGtt                                                                                                                        >  1:226019/1‑151 (MQ=255)                   aTCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGtt                                                                                                                        <  2:655349/150‑1 (MQ=255)                   aTCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGtt                                                                                                                        >  1:318651/1‑150 (MQ=255)                   aTCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGt                                                                                                                         <  1:572465/149‑1 (MQ=255)                       ttGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGttatta                                                                                                                    <  2:741875/150‑1 (MQ=255)                         gATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATc                                                                                                                  >  2:22130/1‑150 (MQ=255)                             ttATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCAccc                                                                                                              <  2:127003/150‑1 (MQ=255)                              tATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCa                                                                                                             <  1:346845/150‑1 (MQ=255)                                      aaCGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTg                                                                                                    >  1:544801/1‑151 (MQ=255)                                         gcgGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCaa                                                                                                 <  1:743183/151‑1 (MQ=255)                                         gcgGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCa                                                                                                  >  2:260604/1‑150 (MQ=255)                                                           tgttgCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGa                                                                                 >  1:596011/1‑149 (MQ=255)                                                              tgCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGc                                                                            >  1:502677/1‑151 (MQ=255)                                                                  gggTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGaa                                                                        <  1:546769/151‑1 (MQ=255)                                                                             gggACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTc                                                             >  2:392438/1‑151 (MQ=255)                                                                                aCTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCaa                                                          <  1:227475/151‑1 (MQ=255)                                                                                       aGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGt                                                   >  1:361872/1‑151 (MQ=255)                                                                                         aCGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTa                                                 <  2:734402/151‑1 (MQ=255)                                                                                         aCGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTa                                                 <  1:656456/151‑1 (MQ=255)                                                                                             aaaGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTAcccc                                             >  2:339436/1‑151 (MQ=255)                                                                                               aGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCaa                                           <  1:745552/151‑1 (MQ=255)                                                                                                      cACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTga                                    <  1:262733/151‑1 (MQ=255)                                                                                                        cTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTgagg                                  >  2:727623/1‑151 (MQ=255)                                                                                                         tATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTgagg                                  >  1:284164/1‑150 (MQ=255)                                                                                                            gCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGaa                              <  1:307095/151‑1 (MQ=255)                                                                                                                    gCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTCAAGACGCAGATTCCAAATGAGGTTACCCCAAAGCTTGAGGAGAACAGCAAAt                      <  2:148988/151‑1 (MQ=255)                                                                                                                        ttttAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGc                   <  1:49709/150‑1 (MQ=255)                                                                                                                         tttAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCg                 >  2:284533/1‑151 (MQ=255)                                                                                                                           tAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCggg               >  1:606456/1‑151 (MQ=255)                                                                                                                            aaGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGt              <  2:318638/151‑1 (MQ=255)                                                                                                                             aGGGAAACGATCTGGGCGCCAGCGCCTGTCGCGTCTGCAAGGTTATTATCACCCAGAAAGATGGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGtt             >  2:13712/1‑151 (MQ=255)                                                                                                                              gggAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGttt            <  1:232827/151‑1 (MQ=255)                                                                                                                                    cGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTg           >  2:477026/1‑146 (MQ=255)                                                                                                                                        cTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGt  >  1:147578/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                   GCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGACGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGT  >  NC_000913/216533‑216818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑