Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,028,476	T→C	G452G (GGT→GGC)	ghxQ →	guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,028,476	0	T	C	100.0%	176.3 / NA	46	G452G (GGT→GGC)	ghxQ	guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/17);  total (29/17)

TCATCGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATT  >  NC_000913/3028328‑3028621                                                                                                                                                     |                                                                                                                                                  tCATCGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTg                                                                                                                                                 >  1:407532/1‑151 (MQ=255)  cATCGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTgg                                                                                                                                                >  1:519361/1‑151 (MQ=255)     cGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCa                                                                                                                                             <  1:341846/151‑1 (MQ=255)     cGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCa                                                                                                                                             <  2:395385/151‑1 (MQ=255)                  aaTATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGAtt                                                                                                                                 <  2:226362/150‑1 (MQ=255)                   atatCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATtc                                                                                                                                >  2:410790/1‑150 (MQ=255)                     atCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATtctc                                                                                                                              <  1:481700/150‑1 (MQ=255)                       cGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCcgc                                                                                                                           >  2:495564/1‑151 (MQ=255)                        gTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCcgcg                                                                                                                          <  1:510943/151‑1 (MQ=255)                         ttACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCgcgc                                                                                                                         >  1:688736/1‑151 (MQ=255)                           aCCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCt                                                                                                                       >  1:724572/1‑151 (MQ=255)                               gTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTc                                                                                                                    >  1:148657/1‑150 (MQ=255)                                     tGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCgg                                                                                                             >  1:127906/1‑151 (MQ=255)                                         gggCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCgg                                                                                                             >  2:114259/1‑147 (MQ=255)                                               tgtgACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGAtttcttt                                                                                                   >  2:571570/1‑151 (MQ=255)                                                gtgACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGAtttcttt                                                                                                   <  1:422751/150‑1 (MQ=255)                                                    cacTCGCCGTAGGCCGCGTCTTTGCACGCGAATGGCAAACGCTGAATATTGGCACGGCGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGCGGCTGGGCAATCTCATAGATTCTCCGCGCCTTTCAGCGCGCAGATTTCTTTCag                                                                                                >  2:346376/1‑149 (MQ=255)                                                     acTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCag                                                                                                >  2:699649/1‑148 (MQ=255)                                                       tGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAgg                                                                                           >  2:83882/1‑151 (MQ=255)                                                              aGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATtctc                                                                                     >  2:197259/1‑150 (MQ=255)                                                              aGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCa                                                                                    <  1:631693/151‑1 (MQ=255)                                                                  cgcgTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGc                                                                                <  2:175726/151‑1 (MQ=255)                                                                          tGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGcaac                                                                         >  1:607452/1‑150 (MQ=255)                                                                           gCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGcaaca                                                                        >  2:645277/1‑150 (MQ=255)                                                                           gCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGcaac                                                                         >  2:172950/1‑149 (MQ=255)                                                                             aCGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATa                                                                     <  2:466906/151‑1 (MQ=255)                                                                               gcgAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACg                                                                   >  1:543352/1‑151 (MQ=255)                                                                                 gAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACgct                                                                 >  1:203135/1‑151 (MQ=255)                                                                                   aTGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACgctgc                                                               >  2:67262/1‑151 (MQ=255)                                                                                         aaaGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCgg                                                         >  1:603877/1‑151 (MQ=255)                                                                                         aaaGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCgg                                                         >  2:172459/1‑151 (MQ=255)                                                                                         aaaGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCgg                                                         >  2:228741/1‑151 (MQ=255)                                                                                            gCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACt                                                      >  1:108927/1‑151 (MQ=255)                                                                                               gAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTc                                                     <  1:243518/149‑1 (MQ=255)                                                                                               gAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGc                                                   >  2:252729/1‑151 (MQ=255)                                                                                                 atatTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCCCCGCTGGCAACAATACGCTGCTGGCGGACTCGCCt                                                 <  1:269239/151‑1 (MQ=255)                                                                                                   atTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTg                                                >  2:608151/1‑150 (MQ=255)                                                                                                              gATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTc                                     <  1:541632/150‑1 (MQ=255)                                                                                                                   ttACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTa                                 >  1:547945/1‑149 (MQ=255)                                                                                                                     aCTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCa                             <  2:325320/151‑1 (MQ=255)                                                                                                                        gCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACt                          <  2:519337/151‑1 (MQ=255)                                                                                                                           gCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCa                       <  1:402339/151‑1 (MQ=255)                                                                                                                                ggTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGcc                  >  2:174282/1‑151 (MQ=255)                                                                                                                                         ttACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTgg          <  2:531510/150‑1 (MQ=255)                                                                                                                                            cgcgGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCa       >  1:270538/1‑150 (MQ=255)                                                                                                                                               gggTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGAt   <  1:723466/151‑1 (MQ=255)                                                                                                                                                ggtggcTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGAtt  <  2:543324/151‑1 (MQ=255)                                                                                                                                                ggtggcTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGAtt  >  2:600980/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TCATCGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATT  >  NC_000913/3028328‑3028621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑