Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,028,861 | G→A | A51V (GCG→GTG) | ygfS ← | putative 4Fe‑4S ferredoxin‑type oxidoreductase subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,028,861 | 0 | G | A | 98.2% | 220.1 / NA | 57 | A51V (GCG→GTG) | ygfS | putative 4Fe‑4S ferredoxin‑type oxidoreductase subunit |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/34); minor base T (0/1); total (22/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTCCAAACGGGCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGAT > NC_000913/3028714‑3029007 | aTTCCAAACGGGCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCacac > 2:70678/1‑151 (MQ=255) aaaCGGGCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATg > 2:337874/1‑151 (MQ=255) ggCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTc < 2:630190/151‑1 (MQ=255) acgcacTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAg < 2:552026/151‑1 (MQ=255) gcacTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTc > 2:172639/1‑151 (MQ=255) cacTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTc < 2:276681/150‑1 (MQ=255) gACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGt > 2:47256/1‑151 (MQ=255) gCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCGAGTCGCTGTAc > 2:88083/1‑150 (MQ=255) gCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTAcc > 2:123035/1‑151 (MQ=255) catctctGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCt < 1:228750/148‑1 (MQ=255) aaCTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCtt < 2:356222/151‑1 (MQ=255) aCTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTCCCTTc > 2:730613/1‑151 (MQ=255) gACAGCCCATACGACGGGCAGAATTGGTCTGCTCCACCTGCTCTACCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCg < 2:352572/151‑1 (MQ=255) gACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGGCGCTGTACCTTCAGc < 1:298640/149‑1 (MQ=255) cAGCCAATACAACGGGCAGAATTGGTCTGCGCCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCTGCTCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCgg < 2:417411/150‑1 (MQ=255) cAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCggg > 2:500211/1‑151 (MQ=255) cAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCggg > 2:662201/1‑151 (MQ=255) cAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCgg < 2:26480/150‑1 (MQ=255) aGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCgggg > 1:649183/1‑151 (MQ=255) cAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTaa < 2:471413/151‑1 (MQ=255) cAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTaa < 1:571602/151‑1 (MQ=255) aaTACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTaaa < 1:91966/151‑1 (MQ=255) aaTACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTaaa < 2:544036/151‑1 (MQ=255) aTACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTaaaa < 2:176865/151‑1 (MQ=255) cGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACAt < 1:557178/151‑1 (MQ=255) gggCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATc < 1:449165/151‑1 (MQ=255) cAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGc < 1:252740/151‑1 (MQ=255) aaTTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCAtt < 2:42850/151‑1 (MQ=255) aaTTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCAtt < 2:607416/151‑1 (MQ=255) tGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCa > 1:62503/1‑150 (MQ=255) gTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACt > 2:676394/1‑151 (MQ=255) gCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTg > 1:729399/1‑151 (MQ=255) tGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGa > 1:765739/1‑149 (MQ=255) gCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAgg < 1:210775/151‑1 (MQ=255) gCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAg < 2:765388/150‑1 (MQ=255) cGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTg < 1:472209/151‑1 (MQ=255) gCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTg > 1:339104/1‑150 (MQ=255) cccATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAg < 2:291856/151‑1 (MQ=255) aTCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCAAACTGATGGCACATCACCGGCACAATGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCg > 1:738007/1‑150 (MQ=255) cAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACcaca < 1:728892/151‑1 (MQ=255) aGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCTCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACcacac < 1:346390/151‑1 (MQ=255) cccACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTa < 2:329068/151‑1 (MQ=255) cACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACt > 2:419262/1‑151 (MQ=255) gggCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTcac > 1:489740/1‑151 (MQ=255) aaGCGCCAACACACGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGt > 2:536038/1‑151 (MQ=255) ccAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGc > 1:260735/1‑151 (MQ=255) acacAAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGc < 2:688694/151‑1 (MQ=255) cacaAGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGcc > 1:162346/1‑151 (MQ=255) aaGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAAt < 1:699692/151‑1 (MQ=255) aGGGGCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATa < 2:686703/151‑1 (MQ=255) ggCGTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAg < 1:83883/151‑1 (MQ=255) gTTTTCACACTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCa < 2:270527/151‑1 (MQ=255) acTGATGGCACATCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGAtt > 2:372876/1‑151 (MQ=255) cacaTCACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATaa < 1:272473/151‑1 (MQ=255) cACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGcc < 2:215857/120‑1 (MQ=255) cACCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGcc > 1:215865/1‑120 (MQ=255) aCCGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACg < 2:724528/151‑1 (MQ=255) cGGCACACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGAt < 1:495588/151‑1 (MQ=255) | ATTCCAAACGGGCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGCCAACACAAGGGGCGTTTTCACACTGATGGCACATCACCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGAT > NC_000913/3028714‑3029007 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |