Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,030,937	T→C	intergenic (‑6/‑430)	ygfT ← / → uacT	putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit/uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,030,937	0	T	C	100.0%	125.3 / NA	36	intergenic (‑6/‑430)	ygfT/uacT	putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit/uric acid permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/31);  total (5/31)

CCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCATCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACA  >  NC_000913/3030789‑3031083                                                                                                                                                     |                                                                                                                                                   ccTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAACCTTATTCATTTGCAccc                                                                                                                                                  <  2:34079/151‑1 (MQ=255)    ttCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCAcccc                                                                                                                                                 <  2:415929/149‑1 (MQ=255)    ttCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCt                                                                                                                                                <  1:510998/150‑1 (MQ=255)     tCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCtt                                                                                                                                               <  2:707330/150‑1 (MQ=255)     tCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCtt                                                                                                                                               <  1:693430/150‑1 (MQ=255)      cccTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTc                                                                                                                                             <  2:368633/151‑1 (MQ=255)          aCAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACACCTTTCAttt                                                                                                                                         <  2:726833/151‑1 (MQ=255)               gTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGa                                                                                                                                    <  1:396451/151‑1 (MQ=255)                 ggATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGt                                                                                                                                  <  2:536030/151‑1 (MQ=255)                      cgcgGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGt                                                                                                                             <  1:423872/151‑1 (MQ=255)                            cGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCACAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGtcttct                                                                                                                        <  1:463025/150‑1 (MQ=255)                                 gTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAAtt                                                                                                                  <  1:211194/151‑1 (MQ=255)                                   cACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAGGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAAttt                                                                                                                 <  2:315557/150‑1 (MQ=255)                                       gtgACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTcgcg                                                                                                             <  1:621927/150‑1 (MQ=255)                                       gtgACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTcgc                                                                                                              <  1:223350/149‑1 (MQ=255)                                           cTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGc                                                                                                         >  1:437827/1‑150 (MQ=255)                                             cAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCa                                                                                                        <  1:653373/149‑1 (MQ=255)                                              aGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATa                                                                                                     <  1:61467/151‑1 (MQ=255)                                               gCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATaa                                                                                                    <  1:599366/151‑1 (MQ=255)                                                  gCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACg                                                                                                 <  1:674943/151‑1 (MQ=255)                                                     aGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATTCATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGc                                                                                              <  1:27257/151‑1 (MQ=255)                                                             tGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCatatat                                                                                        <  2:60964/149‑1 (MQ=255)                                                                  gtgtgCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAg                                                                                 <  2:307994/151‑1 (MQ=255)                                                                   tgtgCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGc                                                                                >  2:698714/1‑151 (MQ=255)                                                                      gCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATg                                                                             <  2:529974/151‑1 (MQ=255)                                                                               caGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGtttt                                                                     <  2:437806/150‑1 (MQ=255)                                                                               caGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTa                                                                    <  2:484501/151‑1 (MQ=255)                                                                                 ggCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAAt                                                                  <  2:541260/151‑1 (MQ=255)                                                                                    aaTTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATtat                                                               <  2:305097/151‑1 (MQ=255)                                                                                    aaTTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATtat                                                               <  2:220130/151‑1 (MQ=255)                                                                                                aTGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACt                                                   >  1:167182/1‑151 (MQ=255)                                                                                                aTGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACt                                                   <  2:556503/151‑1 (MQ=255)                                                                                                       ccTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCa                                            <  1:393855/151‑1 (MQ=255)                                                                                                            aCATTCCGCAGCTTCCTGAACAATAATCTAATTCATTTGTACCCCTCACATTTAATGAATTACGTCTTCCCTAATTTCTCGGTGCAATAATCGGGCATATATTAGGTAGCATGTGCGGATTAAATATAATTTTGCGACGGAgaacaagt                                         <  2:441855/149‑8 (MQ=255)                                                                                                                         tCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATc                          >  1:56107/1‑151 (MQ=255)                                                                                                                                          aTTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCatta          <  2:120640/150‑1 (MQ=255)                                                                                                                                            tCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTAttt       >  2:202888/1‑151 (MQ=255)                                                                                                                                                  gCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACa  <  2:646619/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCATCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACA  >  NC_000913/3030789‑3031083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑