Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,474	T→A	I245F (ATC→TTC)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,474	0	T	A	100.0%	206.0 / NA	53	I245F (ATC→TTC)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (33/20);  total (33/20)

CGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGAT  >  NC_000913/3035330‑3035614                                                                                                                                                 |                                                                                                                                             cGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGt                                                                                                                                        >  1:536510/1‑151 (MQ=255)  gATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTc                                                                                                                                       <  2:194882/151‑1 (MQ=255)     gTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCggg                                                                                                                                    >  1:350206/1‑151 (MQ=255)       cGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGa                                                                                                                                   <  2:36051/150‑1 (MQ=255)       cGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGAt                                                                                                                                  >  1:350742/1‑151 (MQ=255)            ttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTggg                                                                                                                             >  1:360110/1‑151 (MQ=255)            ttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTgg                                                                                                                              <  2:116107/150‑1 (MQ=255)             tGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTggg                                                                                                                             <  1:566757/150‑1 (MQ=255)                     tCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATa                                                                                                                        <  2:700444/147‑1 (MQ=255)                           gCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAg                                                                                                              <  2:191998/151‑1 (MQ=255)                            ccTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGc                                                                                                                >  1:431942/1‑148 (MQ=255)                             cTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGc                                                                                                                >  2:40078/1‑147 (MQ=255)                               gTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGAtt                                                                                                           <  2:605834/150‑1 (MQ=255)                               gTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGAt                                                                                                            <  2:49580/149‑1 (MQ=255)                                 ttCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCgg                                                                                                        >  1:28030/1‑151 (MQ=255)                                 ttCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCg                                                                                                         <  2:671700/150‑1 (MQ=255)                                  tctcGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGCTCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGCTGTGGGTAATACGCACCGCAGATTCGGt                                                                                                       >  2:359/1‑151 (MQ=255)                                     cGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGt                                                                                                       <  2:569889/148‑1 (MQ=255)                                        cATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGtt                                                                                                 <  1:6626/151‑1 (MQ=255)                                         aTTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGtt                                                                                                 >  1:504225/1‑150 (MQ=255)                                            tttCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACg                                                                                             >  1:245525/1‑151 (MQ=255)                                                    gCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAcc                                                                                     >  1:603149/1‑151 (MQ=255)                                                     cATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccg                                                                                    >  2:51917/1‑151 (MQ=255)                                                         tcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcccg                                                                                >  1:621427/1‑151 (MQ=255)                                                           cAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCcgcg                                                                              >  1:56300/1‑151 (MQ=255)                                                                 aTAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAc                                                                        >  1:5322/1‑151 (MQ=255)                                                                    aaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTg                                                                     >  1:460989/1‑151 (MQ=255)                                                                       cTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCg                                                                   >  1:509654/1‑150 (MQ=255)                                                                         tCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCTTTCTGGCACTGGGTCTCGAACCCGGTCGGGATGTGGGTAATACGCTCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCTAt                                                                 <  2:680223/150‑1 (MQ=255)                                                                             tttCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACg                                                            >  1:536281/1‑151 (MQ=255)                                                                                  tCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAAt                                                       >  2:596571/1‑151 (MQ=255)                                                                                       ttCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATgcgc                                                   >  2:410827/1‑150 (MQ=255)                                                                                        tCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCa                                                  <  1:515940/150‑1 (MQ=255)                                                                                            ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATcc                                             >  1:221099/1‑151 (MQ=255)                                                                                            ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATcc                                             >  1:686407/1‑151 (MQ=255)                                                                                            ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATcc                                             <  2:291376/151‑1 (MQ=255)                                                                                             gCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATccg                                            >  2:684453/1‑151 (MQ=255)                                                                                               cTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATccgcc                                          >  2:467923/1‑151 (MQ=255)                                                                                                   tCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGt                                      >  1:464095/1‑151 (MQ=255)                                                                                                      ttGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTg                                    <  2:321762/150‑1 (MQ=255)                                                                                                          tCTTGTGCTGGGAACGGTCGTTATGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAttt                                <  2:587974/150‑1 (MQ=255)                                                                                                             tgtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCg                              <  2:616073/149‑1 (MQ=255)                                                                                                                gCTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatc                         >  1:612234/1‑151 (MQ=255)                                                                                                                 cTGGGAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatc                         >  1:318327/1‑150 (MQ=255)                                                                                                                   gggAACGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaa                       <  1:363165/150‑1 (MQ=255)                                                                                                                        cGGTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcat                 <  2:269855/151‑1 (MQ=255)                                                                                                                         ggTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcat                 <  2:305455/150‑1 (MQ=255)                                                                                                                          gTCGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatc                >  1:695904/1‑150 (MQ=255)                                                                                                                            cGTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGCTTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatcat              >  2:156950/1‑150 (MQ=255)                                                                                                                             gTTCTGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatcatc             >  1:418675/1‑150 (MQ=255)                                                                                                                                 tGGCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACtt        >  1:656842/1‑151 (MQ=255)                                                                                                                                   gCACTGGGTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTcc      >  1:353074/1‑151 (MQ=255)                                                                                                                                        gggTCACGAACCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGAt  >  1:391011/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                             CGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGAT  >  NC_000913/3035330‑3035614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑