Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,161,711	A→G	V320A (GTG→GCG)	ftsP ←	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,161,711	0	A	G	100.0%	168.9 / NA	47	V320A (GTG→GCG)	ftsP	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/25);  total (22/25)

ACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTGCCAG  >  NC_000913/3161565‑3161853                                                                                                                                                   |                                                                                                                                               acaGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGcc                                                                                                                                              >  1:489275/1‑149 (MQ=255)  caGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGTCAAAAGGCCGGTTGGGCGCAGCGTTAGCGCCAgg                                                                                                                                           >  2:69388/1‑151 (MQ=255)  caGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAgg                                                                                                                                           >  2:210720/1‑151 (MQ=255)   aGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAggg                                                                                                                                          <  1:476660/151‑1 (MQ=255)       gTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAg                                                                                                                                       <  1:702153/150‑1 (MQ=255)         ccATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGaa                                                                                                                                     <  1:545335/150‑1 (MQ=255)                 aCCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAAt                                                                                                                             <  1:557656/150‑1 (MQ=255)                  cccGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATAc                                                                                                                           >  1:362189/1‑151 (MQ=255)                   ccGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACt                                                                                                                          >  2:490253/1‑151 (MQ=255)                    cGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTg                                                                                                                         >  2:665432/1‑151 (MQ=255)                            caCCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTc                                                                                                                 <  2:18550/151‑1 (MQ=255)                              cccAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCaa                                                                                                               <  1:668785/151‑1 (MQ=255)                                  gACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAaagaa                                                                                                           <  2:468470/151‑1 (MQ=255)                                      gATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGc                                                                                                         <  1:191196/149‑1 (MQ=255)                                         atCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGa                                                                                                    <  2:169181/151‑1 (MQ=255)                                          tCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGa                                                                                                    >  2:159261/1‑150 (MQ=255)                                           cgcgACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAAt                                                                                                  <  1:756271/151‑1 (MQ=255)                                                 tGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGGAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATc                                                                                            >  1:541332/1‑151 (MQ=255)                                                  gCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATca                                                                                           <  1:222647/151‑1 (MQ=255)                                                     aaTTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATcaac                                                                                         >  1:521194/1‑150 (MQ=255)                                                        tGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATg                                                                                     >  2:487199/1‑151 (MQ=255)                                                               ccAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTcgccg                                                                              <  2:22116/151‑1 (MQ=255)                                                                   ccATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTc                                                                          >  2:428003/1‑151 (MQ=255)                                                                      tGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGcc                                                                       >  1:95475/1‑151 (MQ=255)                                                                                 ggCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCg                                                            >  1:390558/1‑151 (MQ=255)                                                                                     gcaAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGacac                                                        >  1:502003/1‑151 (MQ=255)                                                                                        aGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTc                                                     <  2:520863/151‑1 (MQ=255)                                                                                                 gAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGtt                                            >  2:604845/1‑151 (MQ=255)                                                                                                  aaGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGt                                             >  2:739672/1‑149 (MQ=255)                                                                                                        gtctgtGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCat                                      <  2:169154/150‑1 (MQ=255)                                                                                                         tctgtGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCatat                                    >  1:660229/1‑151 (MQ=255)                                                                                                           tgtgACCAGCGGCAGAAGGCCGGTTGGGCGCGGCGTTAGCGCCAGGGTAGAAGCCAGAACACTGGATGGCTCAAAGAGGCTACGAATACGATCAACAATGGTCGCGGCGGCGCCACAGGTGATCGACGCTTTATCGCCGTTTCTCata                                     <  2:86665/148‑1 (MQ=255)                                                                                                            gtgACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCatat                                    <  1:455926/148‑1 (MQ=255)                                                                                                            gtgACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATcc                                  <  1:314175/150‑1 (MQ=255)                                                                                                            gtgACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATcc                                  <  1:23413/150‑1 (MQ=255)                                                                                                            gtgACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATc                                   <  2:710747/149‑1 (MQ=255)                                                                                                                    cGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGaa                           <  2:31082/149‑1 (MQ=255)                                                                                                                    cGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAAtc                         <  2:374957/151‑1 (MQ=255)                                                                                                                         gAAGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTcgcg                    >  1:411876/1‑151 (MQ=255)                                                                                                                          aaGGCCGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCgcgg                   >  2:227371/1‑151 (MQ=255)                                                                                                                              ccGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCtt                                                                           <  2:372663/91‑1 (MQ=255)                                                                                                                              ccGGTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCtt                                                                           >  1:372679/1‑91 (MQ=255)                                                                                                                                 gTTGGGCGCAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGc            >  2:655672/1‑151 (MQ=255)                                                                                                                                      gcgcAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGt       <  1:159268/151‑1 (MQ=255)                                                                                                                                       cgcAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTg      <  1:339196/151‑1 (MQ=255)                                                                                                                                        gcAGCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTGc     >  2:433882/1‑151 (MQ=255)                                                                                                                                           gCGTTAGCGCCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTGCCAg  <  2:327899/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               ACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTTCATCGCCGTTGCTCATATCCACCAGAATCTCGCGGCGCTCGCCCGGTGCCAG  >  NC_000913/3161565‑3161853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑