Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,166,544 G→A T392I (ACC→ATC)  ygiS ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,166,5440GA98.2% 221.4 / NA 57T392I (ACC→ATC) ygiSputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (22/34);  minor base C (1/0);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACGTCGCATCCCACGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGGTCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCT  >  NC_000913/3166405‑3166689
                                                                                                                                           |                                                                                                                                                 
gTACGTCGCATCCCACGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATg                                                                                                                                         <  2:701080/150‑1 (MQ=255)
 tACGTCGCATCCCACGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGc                                                                                                                                        >  1:358635/1‑150 (MQ=255)
  aCGTCGCATCCCACGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCa                                                                                                                                       <  1:355847/150‑1 (MQ=255)
            ccACGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACttg                                                                                                                            <  2:741207/151‑1 (MQ=255)
            ccACGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACttg                                                                                                                            <  1:515292/151‑1 (MQ=255)
              aCGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACttgtt                                                                                                                          <  1:586884/151‑1 (MQ=255)
              aCGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACttgtt                                                                                                                          >  1:452222/1‑151 (MQ=255)
                 aCTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACttgtt                                                                                                                          >  2:11505/1‑148 (MQ=255)
                  cTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTtgttgt                                                                                                                        >  2:289914/1‑149 (MQ=255)
                   tGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGCTCTTTTCATGCAGATCGTACttgttg                                                                                                                         >  2:299455/1‑147 (MQ=255)
                      cGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAAc                                                                                                                   >  1:415189/1‑150 (MQ=255)
                       gagaCAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAAc                                                                                                                   >  2:99511/1‑149 (MQ=255)
                        agaCAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGc                                                                                                                <  2:463599/151‑1 (MQ=255)
                        agaCAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGc                                                                                                                >  2:104029/1‑151 (MQ=255)
                            aGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCaa                                                                                                            <  1:417986/151‑1 (MQ=255)
                              cATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAg                                                                                                          <  2:178640/151‑1 (MQ=255)
                               aTGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGc                                                                                                         >  2:666529/1‑151 (MQ=255)
                                   aaTCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTTTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGt                                                                                                       >  2:7747/1‑149 (MQ=255)
                                   aaTCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAg                                                                                                     <  1:518230/151‑1 (MQ=255)
                                       aCCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGa                                                                                                 <  1:165090/151‑1 (MQ=255)
                                        ccGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGAt                                                                                                >  1:673202/1‑151 (MQ=255)
                                          ggCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATg                                                                                               >  1:678658/1‑150 (MQ=255)
                                           gCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATgag                                                                                             <  1:486687/151‑1 (MQ=255)
                                              cgtcgGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAgg                                                                                          >  1:515514/1‑151 (MQ=255)
                                               gtcgGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGc                                                                                         <  2:673162/151‑1 (MQ=255)
                                               gtcgGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGc                                                                                         <  2:234419/151‑1 (MQ=255)
                                                   ggCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGcgtcg                                                                                     <  2:330897/151‑1 (MQ=255)
                                                      aTCACGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATTAGAGGCGTCGTAt                                                                                  >  2:268766/1‑151 (MQ=255)
                                                              aGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTg                                                                          <  2:221760/151‑1 (MQ=255)
                                                                  tttCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTc                                                                      >  1:431479/1‑151 (MQ=255)
                                                                   ttCCACTCCTTTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGAAAACGCTTTCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGAGGAGAGGCGCCGTATCCCGCCTGTTTc                                                                      <  2:635218/150‑1 (MQ=255)
                                                                   ttCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTc                                                                      <  1:691201/150‑1 (MQ=255)
                                                                          ccATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAgg                                                               <  1:30316/150‑1 (MQ=255)
                                                                            aTTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCtt                                                            <  1:85193/151‑1 (MQ=255)
                                                                               gtgCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCtttt                                                          <  2:46433/150‑1 (MQ=255)
                                                                                     aGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATcg                                                   <  2:623056/151‑1 (MQ=255)
                                                                                     aGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATcg                                                   >  2:173517/1‑151 (MQ=255)
                                                                                     aGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATcg                                                   >  1:310323/1‑151 (MQ=255)
                                                                                      gCGTCACCTGTGCACCAAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTACCCCGCCTGTTTCAGCAAGGCTTTTGCCATcgc                                                  <  2:495774/151‑1 (MQ=255)
                                                                                       cGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATcgcg                                                 <  1:630405/151‑1 (MQ=255)
                                                                                            ccTGTGCCCCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGAcgcg                                            <  2:342006/151‑1 (MQ=255)
                                                                                                 gCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGct                                          <  1:658777/148‑1 (MQ=255)
                                                                                                      cAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCAtt                                  <  1:227968/151‑1 (MQ=255)
                                                                                                       aGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTg                                 <  2:162263/151‑1 (MQ=255)
                                                                                                        gCCATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTgg                                >  2:652227/1‑151 (MQ=255)
                                                                                                          cATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCt                              <  2:495184/151‑1 (MQ=255)
                                                                                                          cATTTTTTCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCt                              >  2:546986/1‑151 (MQ=255)
                                                                                                              ttttCCATTCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCtttt                           <  2:114238/150‑1 (MQ=255)
                                                                                                                     ttCGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTc                    >  1:582374/1‑150 (MQ=255)
                                                                                                                       cGGAAGACAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATc                 <  1:394918/151‑1 (MQ=255)
                                                                                                                             aCAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAAcgt           <  2:656966/151‑1 (MQ=255)
                                                                                                                              cAACGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAAcgtc          >  1:499945/1‑151 (MQ=255)
                                                                                                                                aCGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAAcgtcgt        <  2:425188/151‑1 (MQ=255)
                                                                                                                                 cGCTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACgtcgtc       <  2:415168/151‑1 (MQ=255)
                                                                                                                                   cTATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTcgc     >  2:43019/1‑151 (MQ=255)
                                                                                                                                    tATCGCGATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTcgcg    >  2:304445/1‑151 (MQ=255)
                                                                                                                                       cgcgATCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCt  <  2:87316/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
GTACGTCGCATCCCACGACTGCCGAGACAGCATGAAATCACCGGCTCGTCGGGCATCAAGATAGGTTTTCCACTCCATTGTGCGCAGCGTCACCTGTGCACCCAGCCATTTTTTCCATTCGGAAGACAACGCTATCGCGGTCTTTTCATGCAGATCGTACTTGTTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCT  >  NC_000913/3166405‑3166689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑