Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,280,131	A→G	E406E (GAA→GAG)	kbaZ →	tagatose 6‑phosphate aldolase 1, kbaZ subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,280,131	0	A	G	100.0%	199.7 / NA	54	E406E (GAA→GAG)	kbaZ	tagatose 6‑phosphate aldolase 1, kbaZ subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/27);  total (27/27)

ACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTC  >  NC_000913/3279983‑3280277                                                                                                                                                     |                                                                                                                                                   aCTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATGCCGCTGCGGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCt                                                                                                                                                  <  2:409141/151‑1 (MQ=255)  cTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTc                                                                                                                                                 >  2:60853/1‑151 (MQ=255)      ccGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCAtt                                                                                                                                              >  2:515655/1‑150 (MQ=255)      ccGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTa                                                                                                                                             <  1:37108/151‑1 (MQ=255)       cGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTAt                                                                                                                                            >  1:443521/1‑151 (MQ=255)           cAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAAc                                                                                                                                        >  1:704082/1‑151 (MQ=255)              ccAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCat                                                                                                                                     >  2:344440/1‑151 (MQ=255)               cAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCata                                                                                                                                    <  1:724585/151‑1 (MQ=255)                 gATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATAtt                                                                                                                                  <  2:28282/151‑1 (MQ=255)                 gATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATAtt                                                                                                                                  <  2:367426/151‑1 (MQ=255)                 gATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATAtt                                                                                                                                  <  1:763346/151‑1 (MQ=255)                    tgatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCa                                                                                                                                <  1:140341/150‑1 (MQ=255)                            cTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATcc                                                                                                                        >  2:587583/1‑150 (MQ=255)                              ttCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCt                                                                                                                       <  1:438886/149‑1 (MQ=255)                                            aCGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcacaca                                                                                                       <  2:292457/151‑1 (MQ=255)                                             cGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcacac                                                                                                        >  1:719224/1‑149 (MQ=255)                                              gTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGCCATCCTGGCGCAGTACCACACAGc                                                                                                     >  1:592265/1‑151 (MQ=255)                                              gTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGc                                                                                                     >  2:688732/1‑151 (MQ=255)                                                  tCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgt                                                                                                 >  1:625534/1‑151 (MQ=255)                                                   cTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgtg                                                                                                >  1:170043/1‑151 (MQ=255)                                                     ggCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgt                                                                                                 <  2:242653/148‑1 (MQ=255)                                                      gCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAg                                                                                             <  2:97353/151‑1 (MQ=255)                                                                cAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGc                                                                                   <  2:548380/151‑1 (MQ=255)                                                                 aaTTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCa                                                                                  <  2:26488/151‑1 (MQ=255)                                                                     ccgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATGAGCAAAAc                                                                              <  1:644688/151‑1 (MQ=255)                                                                     ccgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCaaaa                                                                               <  1:378906/150‑1 (MQ=255)                                                                      cgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAAc                                                                              >  1:49125/1‑150 (MQ=255)                                                                      cgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAAc                                                                              >  1:340412/1‑150 (MQ=255)                                                                             gctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAgg                                                                       >  2:307849/1‑150 (MQ=255)                                                                             gctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGa                                                                      <  2:308213/151‑1 (MQ=255)                                                                                  tCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACg                                                                 >  1:115808/1‑151 (MQ=255)                                                                                       cAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATg                                                            <  2:98469/151‑1 (MQ=255)                                                                                         gTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGcc                                                          <  1:619389/151‑1 (MQ=255)                                                                                                 cGCTGCAGTACGTGAAAGTTAGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAAGACATCCTGGCGCAGGACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAata                                                     >  2:83115/1‑148 (MQ=255)                                                                                                     gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGtttt                                              >  2:641675/1‑151 (MQ=255)                                                                                                     gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGttt                                               <  2:761485/150‑1 (MQ=255)                                                                                                     gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGtt                                                <  2:707434/149‑1 (MQ=255)                                                                                                           cGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCg                                        <  2:374324/151‑1 (MQ=255)                                                                                                           cGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCg                                        <  1:630311/151‑1 (MQ=255)                                                                                                              gAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGAt                                     >  1:618648/1‑151 (MQ=255)                                                                                                                aaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtg                                   >  1:475995/1‑151 (MQ=255)                                                                                                                 aGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtga                                  <  2:34812/151‑1 (MQ=255)                                                                                                                 aGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtga                                  <  1:421542/151‑1 (MQ=255)                                                                                                                        tCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGc                           >  2:613574/1‑151 (MQ=255)                                                                                                                         ccGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGc                           <  2:208093/150‑1 (MQ=255)                                                                                                                         ccGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCt                          <  2:90475/151‑1 (MQ=255)                                                                                                                             cGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAt                      <  1:196287/151‑1 (MQ=255)                                                                                                                              gAGCTGCAGCCAACGCCAGGGGCGCTCATTATCAACCATATTCAGGACCTCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAACGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAAAgcttgctt                     >  1:520451/1‑151 (MQ=255)                                                                                                                              gAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCGAATATTGTTTTAAGCCGGATTGATGAACGCTTGAtt                     >  1:80577/1‑151 (MQ=255)                                                                                                                               aGCTGCAGCCAACGCCACTGGAGCTCATTAGCAACCATATTCAGGACATCCAGGCGCAGTACCACGCAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAtt                     >  2:118952/1‑150 (MQ=255)                                                                                                                               aGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTc                    >  1:417725/1‑151 (MQ=255)                                                                                                                                                 gggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTc  >  2:64144/1‑151 (MQ=255)                                                                                                                                                 gggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTc  >  2:328609/1‑151 (MQ=255)                                                                                                                                                  ggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTc  >  1:87744/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTC  >  NC_000913/3279983‑3280277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑