Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,343,858	T→C	V160A (GTA→GCA)	lptA →	lipopolysaccharide export ABC transporter periplasmic binding protein; Lipid A binding protein; LPS export and assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,343,858	0	T	C	100.0%	278.9 / NA	73	V160A (GTA→GCA)	lptA	lipopolysaccharide export ABC transporter periplasmic binding protein; Lipid A binding protein; LPS export and assembly protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/37);  total (36/37)

GATGCACTACGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGA  >  NC_000913/3343712‑3344005                                                                                                                                                   |                                                                                                                                                    gATGCACTACGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCaaca                                                                                                                                                 <  1:654975/151‑1 (MQ=255)    gCACTACGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCaacaac                                                                                                                                               <  1:54372/150‑1 (MQ=255)         aCGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGtt                                                                                                                                           <  1:64152/149‑1 (MQ=255)           gAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTgg                                                                                                                                       <  2:49089/151‑1 (MQ=255)           gAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTgg                                                                                                                                       >  1:696749/1‑151 (MQ=255)           gAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTgg                                                                                                                                       >  2:116313/1‑151 (MQ=255)            aaCTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTgg                                                                                                                                       >  2:741329/1‑150 (MQ=255)            aaCTGGCAAAAGATTTTGTCGTTCGGACGGATAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGTGATAAGATCACTTACCTGTGCAAAGCACAGAAAATGGAGGCTTTCAGCGACAAAGTCAAGCGCTCAACATCCGTTCTgg                                                                                                                                       <  2:342845/150‑1 (MQ=255)             aCTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTg                                                                                                                                     >  1:123564/1‑151 (MQ=255)              cTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGc                                                                                                                                    >  1:761388/1‑151 (MQ=255)                ggCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGcc                                                                                                                                   <  1:571243/150‑1 (MQ=255)                  cAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTc                                                                                                                                <  1:89988/151‑1 (MQ=255)                  cAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTc                                                                                                                                <  1:19713/151‑1 (MQ=255)                  cAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTc                                                                                                                                >  1:159826/1‑151 (MQ=255)                   aaaaGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCg                                                                                                                               <  2:233750/151‑1 (MQ=255)                     aaGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGc                                                                                                                              >  1:382483/1‑150 (MQ=255)                         ttttGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTg                                                                                                                         >  2:275840/1‑151 (MQ=255)                                  tCTGAGGGGTAATGCTTATCTGCACCAGGCCGATCGCAACATTAAGTGCGATAAGATCACTTACCTGGGGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGATCTGGTGCCGTCGCAGCTGCAGGGCa                                                                                                                  >  2:10868/1‑149 (MQ=255)                                     gACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACaaaaa                                                                                                              <  1:339640/150‑1 (MQ=255)                                       cGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAaaca                                                                                                            >  2:59185/1‑150 (MQ=255)                                        gggTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGAAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAaacaac                                                                                                          >  1:14369/1‑151 (MQ=255)                                         ggTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAacaaca                                                                                                         <  2:90944/151‑1 (MQ=255)                                               gCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGcc                                                                                                   >  2:318279/1‑151 (MQ=255)                                                  tATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGcc                                                                                                   >  2:548936/1‑148 (MQ=255)                                                  tATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCa                                                                                                  <  2:602947/149‑1 (MQ=255)                                                        cagcagGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCgg                                                                                          >  2:301076/1‑151 (MQ=255)                                                         agcagGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGc                                                                                         >  2:26556/1‑151 (MQ=255)                                                                cGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACagaa                                                                                   <  1:326667/150‑1 (MQ=255)                                                                cGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACaga                                                                                    >  2:315885/1‑149 (MQ=255)                                                                 gATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACaga                                                                                    >  2:67569/1‑148 (MQ=255)                                                                    aGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAgg                                                                              <  1:20187/151‑1 (MQ=255)                                                                    aGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAgg                                                                              <  1:7022/151‑1 (MQ=255)                                                                       aaCATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGt                                                                            >  1:274551/1‑150 (MQ=255)                                                                       aaCATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGt                                                                            >  1:578582/1‑150 (MQ=255)                                                                         cATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaat                                                                         <  1:446619/151‑1 (MQ=255)                                                                         cATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaat                                                                         >  2:73253/1‑151 (MQ=255)                                                                         cATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAG‑AAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaatt                                                                        <  2:537708/151‑1 (MQ=255)                                                                          aTTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaatt                                                                        <  2:36066/151‑1 (MQ=255)                                                                             aaGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaattaat                                                                     <  2:141136/151‑1 (MQ=255)                                                                             aaGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaattaat                                                                     >  1:668778/1‑151 (MQ=255)                                                                             aaGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaattaa                                                                      <  1:79951/150‑1 (MQ=255)                                                                               gggCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTc                                                                   <  2:529982/151‑1 (MQ=255)                                                                                  cGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGtt                                                                <  2:179298/151‑1 (MQ=255)                                                                                     tAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATg                                                             >  2:179939/1‑151 (MQ=255)                                                                                          tCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAAc                                                        >  1:226165/1‑151 (MQ=255)                                                                                           cACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAAc                                                        >  2:279629/1‑150 (MQ=255)                                                                                           cACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACa                                                       <  2:529024/151‑1 (MQ=255)                                                                                                 ccTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACt                                                 >  2:617557/1‑151 (MQ=255)                                                                                                   tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGc                                               >  1:491146/1‑151 (MQ=255)                                                                                                   tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGc                                               >  1:503099/1‑151 (MQ=255)                                                                                                    ggTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGc                                               >  2:420051/1‑150 (MQ=255)                                                                                                       gAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCaaa                                            <  1:182671/150‑1 (MQ=255)                                                                                                       gAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCaa                                             >  2:252073/1‑149 (MQ=255)                                                                                                          agagCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAAc                                        <  1:520474/151‑1 (MQ=255)                                                                                                          agagCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAAc                                        >  2:355936/1‑151 (MQ=255)                                                                                                              cAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTg                                    <  1:611734/151‑1 (MQ=255)                                                                                                              cAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTg                                    <  2:377044/151‑1 (MQ=255)                                                                                                                gAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCa                                  <  1:568950/151‑1 (MQ=255)                                                                                                                 aaaaTGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCa                                  >  1:757698/1‑150 (MQ=255)                                                                                                                  aaaTGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaaa                                <  2:318393/151‑1 (MQ=255)                                                                                                                  aaaTGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaa                                 >  1:567211/1‑150 (MQ=255)                                                                                                                   aaTGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaaaa                               <  2:525293/151‑1 (MQ=255)                                                                                                                    aTGCAGGCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAg                              <  1:420284/151‑1 (MQ=255)                                                                                                                         ggCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCtat                         <  2:180643/151‑1 (MQ=255)                                                                                                                          gCTTTCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCtata                        <  1:659031/151‑1 (MQ=255)                                                                                                                             ttCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATaaa                      <  1:760426/150‑1 (MQ=255)                                                                                                                             ttCAGCGACAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATaa                       <  1:342356/149‑1 (MQ=255)                                                                                                                                     cAAAGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGGAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTcgc             >  2:156100/1‑151 (MQ=255)                                                                                                                                      aaaGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTcgcg            >  2:326272/1‑151 (MQ=255)                                                                                                                                      aaaGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGCCCAAAACCAACAAATGCCAGACCCCGGCTCAGAAGAAGGGTAAGTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCGGAcgc             <  2:169985/150‑1 (MQ=255)                                                                                                                                       aaGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGt           <  1:21956/151‑1 (MQ=255)                                                                                                                                       aaGGCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGt           >  1:47057/1‑151 (MQ=255)                                                                                                                                          gCAAGCGCGCAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGt        >  2:525341/1‑151 (MQ=255)                                                                                                                                                gcgcAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTagaaga  <  2:491123/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GATGCACTACGAACTGGCAAAAGATTTTGTCGTTCTGACGGGTAATGCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGA  >  NC_000913/3343712‑3344005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑