Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,102	A→G	V158A (GTC→GCC)	nanT ←	sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,102	0	A	G	100.0%	123.6 / NA	39	V158A (GTC→GCC)	nanT	sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/20);  total (19/20)

TTACCTGCGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGT  >  NC_000913/3371955‑3372247                                                                                                                                                    |                                                                                                                                                  ttACCTGCGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAacggcg                                                                                                                                                 <  1:434469/150‑1 (MQ=255)  tACCTGCGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACggcggc                                                                                                                                               >  2:6074/1‑151 (MQ=255)                 ctTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAg                                                                                                                                  <  2:129218/149‑1 (MQ=255)                 ctTTCCACTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGTTAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCACAAGACCGGAAACACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGACaaac                                                                                                                                 >  2:487011/1‑148 (MQ=255)                             ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGAAAAATGCCGATAAAGAACAGCGCACGACAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCa                                                                                                                        <  2:244852/147‑1 (MQ=255)                                    cgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTg                                                                                                             <  2:481511/151‑1 (MQ=255)                                    cgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTg                                                                                                             >  2:370512/1‑151 (MQ=255)                                      ggATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGc                                                                                                           >  1:756636/1‑151 (MQ=255)                                        aTGTTTTTACTCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCtt                                                                                                         <  2:713832/151‑1 (MQ=255)                                         tGTTTTTACGCAGCCCGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGAGACGGCGGCCCCCACCGAGAAGCCTGAAATCAAAAAACCACTGGCt                                                                                                          <  2:523320/149‑1 (MQ=255)                                             tttACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGtt                                                                                                     >  2:649041/1‑150 (MQ=255)                                                 cGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCCCCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCa                                                                                                <  1:314292/151‑1 (MQ=255)                                                  gCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGc                                                                                                 >  1:428505/1‑149 (MQ=255)                                                  gCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGc                                                                                                 >  2:144228/1‑149 (MQ=255)                                                    aGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGaa                                                                                                                            <  1:151528/120‑1 (MQ=255)                                                    aGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGaa                                                                                                                            >  2:151521/1‑120 (MQ=255)                                                      ccAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGt                                                                                           >  1:647308/1‑151 (MQ=255)                                                          atagCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGGCCTGAGCGGAAACTGCAGCCCCCACAGAGAAGCCTGCAATCAAAAAACCGCTGGCTTTGTTACGCGGATGTTTTg                                                                                       <  2:478327/149‑1 (MQ=255)                                                          agagCAAAGATGATTGGCAAAATGCCGATAAAGGACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTg                                                                                       >  2:422152/1‑151 (MQ=255)                                                          agagCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTg                                                                                       <  1:273247/151‑1 (MQ=255)                                                             gCAAAGATGATTGCCAAAATGCCAATAACGAACAGCGCACGACAGACCCAGACCGGAGCCACCATGCTATAGACCTGAGCGGCAACGGCGGCTCCCTCAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTggac                                                                                    <  2:96603/151‑3 (MQ=255)                                                                aaGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGc                                                                                 >  1:537387/1‑151 (MQ=255)                                                                         ggCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATg                                                                         <  2:94491/150‑1 (MQ=255)                                                                            aaaaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACAt                                                                     >  1:108544/1‑151 (MQ=255)                                                                            aaaaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACAt                                                                     <  2:482566/151‑1 (MQ=255)                                                                                     aTAACGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGTCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGCTGTTTTGGCCAGCTTTCAATGACATAGGTGgcgc                                                            >  2:327787/1‑151 (MQ=255)                                                                                      tAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCt                                                           >  2:483736/1‑151 (MQ=255)                                                                                         aGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTgg                                                         >  1:390552/1‑150 (MQ=255)                                                                                          gAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGa                                                        >  1:98500/1‑150 (MQ=255)                                                                                           aaCAGCGCACGCCAGCCCCTGACCTGATCCACCAGGCTATAGACATGAGCGGCAACGGCGGCCCCCACATTGAAGCCTGTAATCAAAAAACCACTGGCTTTGTTACGCAGAGGTTTTGTCCAGCTTTCAATGACATAGGTGGCGCTGGaa                                                       <  2:230642/150‑1 (MQ=255)                                                                                                      ccAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCAccc                                           >  1:521922/1‑151 (MQ=255)                                                                                                                aCCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCa                                 <  1:733621/151‑1 (MQ=255)                                                                                                                  cGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATg                               >  1:703979/1‑151 (MQ=255)                                                                                                                       ccaccaGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCCCTGGCTTTTTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGAAACCGTATCCCCCCGCCATCCCCATGCCGAt                          <  2:272104/151‑1 (MQ=255)                                                                                                                         accaGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATg                         >  1:540562/1‑150 (MQ=255)                                                                                                                            agacTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGAcc                      <  2:692240/147‑1 (MQ=255)                                                                                                                            aGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCa                     <  2:463202/151‑1 (MQ=255)                                                                                                                                 ataGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGAc                  <  1:36479/149‑1 (MQ=255)                                                                                                                                 ataGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACg                 >  2:235648/1‑150 (MQ=255)                                                                                                                                 ataGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACg                 <  2:671366/150‑1 (MQ=255)                                                                                                                                               cAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGt  <  2:108542/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TTACCTGCGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGT  >  NC_000913/3371955‑3372247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑